Job ID = 6498402 SRX = SRX495237 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:51:22 prefetch.2.10.7: 1) Downloading 'SRR1198742'... 2020-06-25T23:51:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:53:51 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:53:51 prefetch.2.10.7: 1) 'SRR1198742' was downloaded successfully Read 20636266 spots for SRR1198742/SRR1198742.sra Written 20636266 spots for SRR1198742/SRR1198742.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:33 20636266 reads; of these: 20636266 (100.00%) were unpaired; of these: 206462 (1.00%) aligned 0 times 13986286 (67.78%) aligned exactly 1 time 6443518 (31.22%) aligned >1 times 99.00% overall alignment rate Time searching: 00:08:34 Overall time: 00:08:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3744136 / 20429804 = 0.1833 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:09:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:09:15: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:09:15: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:09:21: 1000000 INFO @ Fri, 26 Jun 2020 09:09:28: 2000000 INFO @ Fri, 26 Jun 2020 09:09:35: 3000000 INFO @ Fri, 26 Jun 2020 09:09:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:09:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:09:45: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:09:45: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:09:48: 5000000 INFO @ Fri, 26 Jun 2020 09:09:52: 1000000 INFO @ Fri, 26 Jun 2020 09:09:56: 6000000 INFO @ Fri, 26 Jun 2020 09:09:59: 2000000 INFO @ Fri, 26 Jun 2020 09:10:03: 7000000 INFO @ Fri, 26 Jun 2020 09:10:07: 3000000 INFO @ Fri, 26 Jun 2020 09:10:10: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:10:14: 4000000 INFO @ Fri, 26 Jun 2020 09:10:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:10:15: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:10:15: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:10:17: 9000000 INFO @ Fri, 26 Jun 2020 09:10:22: 5000000 INFO @ Fri, 26 Jun 2020 09:10:23: 1000000 INFO @ Fri, 26 Jun 2020 09:10:25: 10000000 INFO @ Fri, 26 Jun 2020 09:10:29: 6000000 INFO @ Fri, 26 Jun 2020 09:10:30: 2000000 INFO @ Fri, 26 Jun 2020 09:10:32: 11000000 INFO @ Fri, 26 Jun 2020 09:10:37: 7000000 INFO @ Fri, 26 Jun 2020 09:10:38: 3000000 INFO @ Fri, 26 Jun 2020 09:10:39: 12000000 INFO @ Fri, 26 Jun 2020 09:10:44: 8000000 INFO @ Fri, 26 Jun 2020 09:10:45: 4000000 INFO @ Fri, 26 Jun 2020 09:10:47: 13000000 INFO @ Fri, 26 Jun 2020 09:10:51: 9000000 INFO @ Fri, 26 Jun 2020 09:10:52: 5000000 INFO @ Fri, 26 Jun 2020 09:10:54: 14000000 INFO @ Fri, 26 Jun 2020 09:10:59: 10000000 INFO @ Fri, 26 Jun 2020 09:11:00: 6000000 INFO @ Fri, 26 Jun 2020 09:11:01: 15000000 INFO @ Fri, 26 Jun 2020 09:11:06: 11000000 INFO @ Fri, 26 Jun 2020 09:11:07: 7000000 INFO @ Fri, 26 Jun 2020 09:11:09: 16000000 INFO @ Fri, 26 Jun 2020 09:11:14: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:11:14: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:11:14: #1 total tags in treatment: 16685668 INFO @ Fri, 26 Jun 2020 09:11:14: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:11:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:11:14: 12000000 INFO @ Fri, 26 Jun 2020 09:11:14: #1 tags after filtering in treatment: 16685668 INFO @ Fri, 26 Jun 2020 09:11:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:11:14: #1 finished! INFO @ Fri, 26 Jun 2020 09:11:14: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:11:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:11:15: 8000000 INFO @ Fri, 26 Jun 2020 09:11:15: #2 number of paired peaks: 40 WARNING @ Fri, 26 Jun 2020 09:11:15: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:11:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:11:21: 13000000 INFO @ Fri, 26 Jun 2020 09:11:22: 9000000 INFO @ Fri, 26 Jun 2020 09:11:28: 14000000 INFO @ Fri, 26 Jun 2020 09:11:29: 10000000 INFO @ Fri, 26 Jun 2020 09:11:35: 15000000 INFO @ Fri, 26 Jun 2020 09:11:37: 11000000 INFO @ Fri, 26 Jun 2020 09:11:42: 16000000 INFO @ Fri, 26 Jun 2020 09:11:44: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:11:47: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:11:47: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:11:47: #1 total tags in treatment: 16685668 INFO @ Fri, 26 Jun 2020 09:11:47: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:11:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:11:47: #1 tags after filtering in treatment: 16685668 INFO @ Fri, 26 Jun 2020 09:11:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:11:47: #1 finished! INFO @ Fri, 26 Jun 2020 09:11:47: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:11:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:11:49: #2 number of paired peaks: 40 WARNING @ Fri, 26 Jun 2020 09:11:49: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:11:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:11:51: 13000000 INFO @ Fri, 26 Jun 2020 09:11:58: 14000000 INFO @ Fri, 26 Jun 2020 09:12:05: 15000000 INFO @ Fri, 26 Jun 2020 09:12:11: 16000000 INFO @ Fri, 26 Jun 2020 09:12:16: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:12:16: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:12:16: #1 total tags in treatment: 16685668 INFO @ Fri, 26 Jun 2020 09:12:16: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:12:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:12:16: #1 tags after filtering in treatment: 16685668 INFO @ Fri, 26 Jun 2020 09:12:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:12:16: #1 finished! INFO @ Fri, 26 Jun 2020 09:12:16: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:12:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:12:17: #2 number of paired peaks: 40 WARNING @ Fri, 26 Jun 2020 09:12:17: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:12:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495237/SRX495237.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。