Job ID = 6498399 SRX = SRX495234 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:28:02 prefetch.2.10.7: 1) Downloading 'SRR1198739'... 2020-06-25T23:28:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:31:29 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:31:29 prefetch.2.10.7: 1) 'SRR1198739' was downloaded successfully Read 25111745 spots for SRR1198739/SRR1198739.sra Written 25111745 spots for SRR1198739/SRR1198739.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:59 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 421088 (1.68%) aligned 0 times 20783200 (82.76%) aligned exactly 1 time 3907457 (15.56%) aligned >1 times 98.32% overall alignment rate Time searching: 00:05:59 Overall time: 00:05:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5655656 / 24690657 = 0.2291 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:44:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:44:09: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:44:09: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:44:15: 1000000 INFO @ Fri, 26 Jun 2020 08:44:21: 2000000 INFO @ Fri, 26 Jun 2020 08:44:27: 3000000 INFO @ Fri, 26 Jun 2020 08:44:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:44:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:44:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:44:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:44:40: 5000000 INFO @ Fri, 26 Jun 2020 08:44:45: 1000000 INFO @ Fri, 26 Jun 2020 08:44:46: 6000000 INFO @ Fri, 26 Jun 2020 08:44:52: 2000000 INFO @ Fri, 26 Jun 2020 08:44:53: 7000000 INFO @ Fri, 26 Jun 2020 08:44:59: 3000000 INFO @ Fri, 26 Jun 2020 08:45:00: 8000000 INFO @ Fri, 26 Jun 2020 08:45:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:45:07: 9000000 INFO @ Fri, 26 Jun 2020 08:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:45:08: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:45:08: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:45:12: 5000000 INFO @ Fri, 26 Jun 2020 08:45:14: 10000000 INFO @ Fri, 26 Jun 2020 08:45:15: 1000000 INFO @ Fri, 26 Jun 2020 08:45:19: 6000000 INFO @ Fri, 26 Jun 2020 08:45:21: 11000000 INFO @ Fri, 26 Jun 2020 08:45:22: 2000000 INFO @ Fri, 26 Jun 2020 08:45:26: 7000000 INFO @ Fri, 26 Jun 2020 08:45:27: 12000000 INFO @ Fri, 26 Jun 2020 08:45:29: 3000000 INFO @ Fri, 26 Jun 2020 08:45:33: 8000000 INFO @ Fri, 26 Jun 2020 08:45:34: 13000000 INFO @ Fri, 26 Jun 2020 08:45:36: 4000000 INFO @ Fri, 26 Jun 2020 08:45:40: 9000000 INFO @ Fri, 26 Jun 2020 08:45:41: 14000000 INFO @ Fri, 26 Jun 2020 08:45:43: 5000000 INFO @ Fri, 26 Jun 2020 08:45:47: 10000000 INFO @ Fri, 26 Jun 2020 08:45:48: 15000000 INFO @ Fri, 26 Jun 2020 08:45:50: 6000000 INFO @ Fri, 26 Jun 2020 08:45:54: 11000000 INFO @ Fri, 26 Jun 2020 08:45:55: 16000000 INFO @ Fri, 26 Jun 2020 08:45:57: 7000000 INFO @ Fri, 26 Jun 2020 08:46:01: 12000000 INFO @ Fri, 26 Jun 2020 08:46:02: 17000000 INFO @ Fri, 26 Jun 2020 08:46:05: 8000000 INFO @ Fri, 26 Jun 2020 08:46:08: 13000000 INFO @ Fri, 26 Jun 2020 08:46:09: 18000000 INFO @ Fri, 26 Jun 2020 08:46:12: 9000000 INFO @ Fri, 26 Jun 2020 08:46:15: 14000000 INFO @ Fri, 26 Jun 2020 08:46:16: 19000000 INFO @ Fri, 26 Jun 2020 08:46:17: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:46:17: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:46:17: #1 total tags in treatment: 19035001 INFO @ Fri, 26 Jun 2020 08:46:17: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:46:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:46:17: #1 tags after filtering in treatment: 19035001 INFO @ Fri, 26 Jun 2020 08:46:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:46:17: #1 finished! INFO @ Fri, 26 Jun 2020 08:46:17: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:46:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:46:18: #2 number of paired peaks: 7 WARNING @ Fri, 26 Jun 2020 08:46:18: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:46:18: Process for pairing-model is terminated! INFO @ Fri, 26 Jun 2020 08:46:19: 10000000 cut: /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:46:22: 15000000 INFO @ Fri, 26 Jun 2020 08:46:26: 11000000 INFO @ Fri, 26 Jun 2020 08:46:29: 16000000 INFO @ Fri, 26 Jun 2020 08:46:33: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:46:36: 17000000 INFO @ Fri, 26 Jun 2020 08:46:40: 13000000 INFO @ Fri, 26 Jun 2020 08:46:43: 18000000 INFO @ Fri, 26 Jun 2020 08:46:47: 14000000 INFO @ Fri, 26 Jun 2020 08:46:50: 19000000 INFO @ Fri, 26 Jun 2020 08:46:50: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:46:50: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:46:50: #1 total tags in treatment: 19035001 INFO @ Fri, 26 Jun 2020 08:46:50: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:46:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:46:51: #1 tags after filtering in treatment: 19035001 INFO @ Fri, 26 Jun 2020 08:46:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:46:51: #1 finished! INFO @ Fri, 26 Jun 2020 08:46:51: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:46:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:46:52: #2 number of paired peaks: 7 WARNING @ Fri, 26 Jun 2020 08:46:52: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:46:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:46:54: 15000000 INFO @ Fri, 26 Jun 2020 08:47:00: 16000000 INFO @ Fri, 26 Jun 2020 08:47:07: 17000000 INFO @ Fri, 26 Jun 2020 08:47:13: 18000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 08:47:19: 19000000 INFO @ Fri, 26 Jun 2020 08:47:20: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:47:20: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:47:20: #1 total tags in treatment: 19035001 INFO @ Fri, 26 Jun 2020 08:47:20: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:47:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:47:20: #1 tags after filtering in treatment: 19035001 INFO @ Fri, 26 Jun 2020 08:47:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:47:20: #1 finished! INFO @ Fri, 26 Jun 2020 08:47:20: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:47:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:47:21: #2 number of paired peaks: 7 WARNING @ Fri, 26 Jun 2020 08:47:21: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:47:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495234/SRX495234.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling