Job ID = 6498398 SRX = SRX495233 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:15:27 prefetch.2.10.7: 1) Downloading 'SRR1198738'... 2020-06-25T23:15:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:19:59 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:19:59 prefetch.2.10.7: 1) 'SRR1198738' was downloaded successfully Read 25111745 spots for SRR1198738/SRR1198738.sra Written 25111745 spots for SRR1198738/SRR1198738.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:07:28 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 421074 (1.68%) aligned 0 times 20783125 (82.76%) aligned exactly 1 time 3907546 (15.56%) aligned >1 times 98.32% overall alignment rate Time searching: 00:07:29 Overall time: 00:07:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5654496 / 24690671 = 0.2290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:34:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:34:47: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:34:47: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:34:53: 1000000 INFO @ Fri, 26 Jun 2020 08:34:59: 2000000 INFO @ Fri, 26 Jun 2020 08:35:05: 3000000 INFO @ Fri, 26 Jun 2020 08:35:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:35:17: 5000000 INFO @ Fri, 26 Jun 2020 08:35:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:35:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:35:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:35:24: 6000000 INFO @ Fri, 26 Jun 2020 08:35:24: 1000000 INFO @ Fri, 26 Jun 2020 08:35:31: 7000000 INFO @ Fri, 26 Jun 2020 08:35:31: 2000000 INFO @ Fri, 26 Jun 2020 08:35:38: 3000000 INFO @ Fri, 26 Jun 2020 08:35:38: 8000000 INFO @ Fri, 26 Jun 2020 08:35:44: 4000000 INFO @ Fri, 26 Jun 2020 08:35:45: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:35:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:35:47: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:35:47: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:35:52: 5000000 INFO @ Fri, 26 Jun 2020 08:35:52: 10000000 INFO @ Fri, 26 Jun 2020 08:35:55: 1000000 INFO @ Fri, 26 Jun 2020 08:35:59: 6000000 INFO @ Fri, 26 Jun 2020 08:36:00: 11000000 INFO @ Fri, 26 Jun 2020 08:36:03: 2000000 INFO @ Fri, 26 Jun 2020 08:36:06: 7000000 INFO @ Fri, 26 Jun 2020 08:36:07: 12000000 INFO @ Fri, 26 Jun 2020 08:36:11: 3000000 INFO @ Fri, 26 Jun 2020 08:36:14: 8000000 INFO @ Fri, 26 Jun 2020 08:36:15: 13000000 INFO @ Fri, 26 Jun 2020 08:36:18: 4000000 INFO @ Fri, 26 Jun 2020 08:36:21: 9000000 INFO @ Fri, 26 Jun 2020 08:36:22: 14000000 INFO @ Fri, 26 Jun 2020 08:36:26: 5000000 INFO @ Fri, 26 Jun 2020 08:36:29: 10000000 INFO @ Fri, 26 Jun 2020 08:36:30: 15000000 INFO @ Fri, 26 Jun 2020 08:36:34: 6000000 INFO @ Fri, 26 Jun 2020 08:36:36: 11000000 INFO @ Fri, 26 Jun 2020 08:36:38: 16000000 INFO @ Fri, 26 Jun 2020 08:36:41: 7000000 INFO @ Fri, 26 Jun 2020 08:36:44: 12000000 INFO @ Fri, 26 Jun 2020 08:36:45: 17000000 INFO @ Fri, 26 Jun 2020 08:36:49: 8000000 INFO @ Fri, 26 Jun 2020 08:36:51: 13000000 INFO @ Fri, 26 Jun 2020 08:36:53: 18000000 INFO @ Fri, 26 Jun 2020 08:36:57: 9000000 INFO @ Fri, 26 Jun 2020 08:36:59: 14000000 INFO @ Fri, 26 Jun 2020 08:37:00: 19000000 INFO @ Fri, 26 Jun 2020 08:37:00: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:37:00: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:37:00: #1 total tags in treatment: 19036175 INFO @ Fri, 26 Jun 2020 08:37:00: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:37:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:37:01: #1 tags after filtering in treatment: 19036175 INFO @ Fri, 26 Jun 2020 08:37:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:37:01: #1 finished! INFO @ Fri, 26 Jun 2020 08:37:01: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:37:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:37:02: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 08:37:02: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:37:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:37:04: 10000000 INFO @ Fri, 26 Jun 2020 08:37:06: 15000000 INFO @ Fri, 26 Jun 2020 08:37:12: 11000000 INFO @ Fri, 26 Jun 2020 08:37:13: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:37:20: 12000000 INFO @ Fri, 26 Jun 2020 08:37:21: 17000000 INFO @ Fri, 26 Jun 2020 08:37:27: 13000000 INFO @ Fri, 26 Jun 2020 08:37:28: 18000000 INFO @ Fri, 26 Jun 2020 08:37:35: 19000000 INFO @ Fri, 26 Jun 2020 08:37:35: 14000000 INFO @ Fri, 26 Jun 2020 08:37:35: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:37:35: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:37:35: #1 total tags in treatment: 19036175 INFO @ Fri, 26 Jun 2020 08:37:35: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:37:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:37:36: #1 tags after filtering in treatment: 19036175 INFO @ Fri, 26 Jun 2020 08:37:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:37:36: #1 finished! INFO @ Fri, 26 Jun 2020 08:37:36: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:37:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:37:37: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 08:37:37: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:37:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:37:42: 15000000 INFO @ Fri, 26 Jun 2020 08:37:50: 16000000 INFO @ Fri, 26 Jun 2020 08:37:57: 17000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 08:38:05: 18000000 INFO @ Fri, 26 Jun 2020 08:38:12: 19000000 INFO @ Fri, 26 Jun 2020 08:38:13: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:38:13: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:38:13: #1 total tags in treatment: 19036175 INFO @ Fri, 26 Jun 2020 08:38:13: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:38:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:38:13: #1 tags after filtering in treatment: 19036175 INFO @ Fri, 26 Jun 2020 08:38:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:38:13: #1 finished! INFO @ Fri, 26 Jun 2020 08:38:13: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:38:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:38:15: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 08:38:15: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:38:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495233/SRX495233.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling