Job ID = 6498394 SRX = SRX495229 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:28:31 prefetch.2.10.7: 1) Downloading 'SRR1198734'... 2020-06-25T23:28:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:31:45 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:31:45 prefetch.2.10.7: 1) 'SRR1198734' was downloaded successfully Read 21723799 spots for SRR1198734/SRR1198734.sra Written 21723799 spots for SRR1198734/SRR1198734.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:32 21723799 reads; of these: 21723799 (100.00%) were unpaired; of these: 98926 (0.46%) aligned 0 times 19003263 (87.48%) aligned exactly 1 time 2621610 (12.07%) aligned >1 times 99.54% overall alignment rate Time searching: 00:05:32 Overall time: 00:05:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4158654 / 21624873 = 0.1923 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:44:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:44:03: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:44:03: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:44:09: 1000000 INFO @ Fri, 26 Jun 2020 08:44:16: 2000000 INFO @ Fri, 26 Jun 2020 08:44:23: 3000000 INFO @ Fri, 26 Jun 2020 08:44:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:44:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:44:32: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:44:32: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:44:37: 5000000 INFO @ Fri, 26 Jun 2020 08:44:40: 1000000 INFO @ Fri, 26 Jun 2020 08:44:45: 6000000 INFO @ Fri, 26 Jun 2020 08:44:47: 2000000 INFO @ Fri, 26 Jun 2020 08:44:52: 7000000 INFO @ Fri, 26 Jun 2020 08:44:55: 3000000 INFO @ Fri, 26 Jun 2020 08:44:59: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:45:02: 4000000 INFO @ Fri, 26 Jun 2020 08:45:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:45:02: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:45:02: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:45:07: 9000000 INFO @ Fri, 26 Jun 2020 08:45:10: 5000000 INFO @ Fri, 26 Jun 2020 08:45:11: 1000000 INFO @ Fri, 26 Jun 2020 08:45:15: 10000000 INFO @ Fri, 26 Jun 2020 08:45:19: 6000000 INFO @ Fri, 26 Jun 2020 08:45:20: 2000000 INFO @ Fri, 26 Jun 2020 08:45:23: 11000000 INFO @ Fri, 26 Jun 2020 08:45:27: 7000000 INFO @ Fri, 26 Jun 2020 08:45:29: 3000000 INFO @ Fri, 26 Jun 2020 08:45:31: 12000000 INFO @ Fri, 26 Jun 2020 08:45:36: 8000000 INFO @ Fri, 26 Jun 2020 08:45:38: 4000000 INFO @ Fri, 26 Jun 2020 08:45:39: 13000000 INFO @ Fri, 26 Jun 2020 08:45:44: 9000000 INFO @ Fri, 26 Jun 2020 08:45:47: 5000000 INFO @ Fri, 26 Jun 2020 08:45:47: 14000000 INFO @ Fri, 26 Jun 2020 08:45:52: 10000000 INFO @ Fri, 26 Jun 2020 08:45:56: 15000000 INFO @ Fri, 26 Jun 2020 08:45:56: 6000000 INFO @ Fri, 26 Jun 2020 08:46:00: 11000000 INFO @ Fri, 26 Jun 2020 08:46:04: 16000000 INFO @ Fri, 26 Jun 2020 08:46:05: 7000000 INFO @ Fri, 26 Jun 2020 08:46:09: 12000000 INFO @ Fri, 26 Jun 2020 08:46:12: 17000000 INFO @ Fri, 26 Jun 2020 08:46:14: 8000000 INFO @ Fri, 26 Jun 2020 08:46:15: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:46:15: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:46:15: #1 total tags in treatment: 17466219 INFO @ Fri, 26 Jun 2020 08:46:15: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:46:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:46:16: #1 tags after filtering in treatment: 17466219 INFO @ Fri, 26 Jun 2020 08:46:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:46:16: #1 finished! INFO @ Fri, 26 Jun 2020 08:46:16: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:46:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:46:17: 13000000 INFO @ Fri, 26 Jun 2020 08:46:17: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 08:46:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:46:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:46:23: 9000000 INFO @ Fri, 26 Jun 2020 08:46:25: 14000000 INFO @ Fri, 26 Jun 2020 08:46:32: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:46:33: 15000000 INFO @ Fri, 26 Jun 2020 08:46:41: 16000000 INFO @ Fri, 26 Jun 2020 08:46:41: 11000000 INFO @ Fri, 26 Jun 2020 08:46:49: 17000000 INFO @ Fri, 26 Jun 2020 08:46:50: 12000000 INFO @ Fri, 26 Jun 2020 08:46:52: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:46:52: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:46:52: #1 total tags in treatment: 17466219 INFO @ Fri, 26 Jun 2020 08:46:52: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:46:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:46:52: #1 tags after filtering in treatment: 17466219 INFO @ Fri, 26 Jun 2020 08:46:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:46:52: #1 finished! INFO @ Fri, 26 Jun 2020 08:46:52: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:46:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:46:54: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 08:46:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:46:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:46:58: 13000000 INFO @ Fri, 26 Jun 2020 08:47:07: 14000000 INFO @ Fri, 26 Jun 2020 08:47:15: 15000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 08:47:22: 16000000 INFO @ Fri, 26 Jun 2020 08:47:30: 17000000 INFO @ Fri, 26 Jun 2020 08:47:34: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:47:34: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:47:34: #1 total tags in treatment: 17466219 INFO @ Fri, 26 Jun 2020 08:47:34: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:47:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:47:34: #1 tags after filtering in treatment: 17466219 INFO @ Fri, 26 Jun 2020 08:47:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:47:34: #1 finished! INFO @ Fri, 26 Jun 2020 08:47:34: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:47:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:47:36: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 08:47:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:47:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495229/SRX495229.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling