Job ID = 6498390 SRX = SRX495225 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:43:12 prefetch.2.10.7: 1) Downloading 'SRR1198730'... 2020-06-25T23:43:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:46:10 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:46:10 prefetch.2.10.7: 1) 'SRR1198730' was downloaded successfully Read 25111745 spots for SRR1198730/SRR1198730.sra Written 25111745 spots for SRR1198730/SRR1198730.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:10 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 421061 (1.68%) aligned 0 times 20783187 (82.76%) aligned exactly 1 time 3907497 (15.56%) aligned >1 times 98.32% overall alignment rate Time searching: 00:06:11 Overall time: 00:06:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5654567 / 24690684 = 0.2290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:59:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:59:02: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:59:02: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:59:11: 1000000 INFO @ Fri, 26 Jun 2020 08:59:19: 2000000 INFO @ Fri, 26 Jun 2020 08:59:27: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:59:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:59:32: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:59:32: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:59:36: 4000000 INFO @ Fri, 26 Jun 2020 08:59:40: 1000000 INFO @ Fri, 26 Jun 2020 08:59:44: 5000000 INFO @ Fri, 26 Jun 2020 08:59:48: 2000000 INFO @ Fri, 26 Jun 2020 08:59:53: 6000000 INFO @ Fri, 26 Jun 2020 08:59:56: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:00:01: 7000000 INFO @ Fri, 26 Jun 2020 09:00:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:00:02: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:00:02: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:00:03: 4000000 INFO @ Fri, 26 Jun 2020 09:00:10: 8000000 INFO @ Fri, 26 Jun 2020 09:00:10: 1000000 INFO @ Fri, 26 Jun 2020 09:00:11: 5000000 INFO @ Fri, 26 Jun 2020 09:00:18: 2000000 INFO @ Fri, 26 Jun 2020 09:00:18: 9000000 INFO @ Fri, 26 Jun 2020 09:00:19: 6000000 INFO @ Fri, 26 Jun 2020 09:00:25: 3000000 INFO @ Fri, 26 Jun 2020 09:00:26: 10000000 INFO @ Fri, 26 Jun 2020 09:00:27: 7000000 INFO @ Fri, 26 Jun 2020 09:00:33: 4000000 INFO @ Fri, 26 Jun 2020 09:00:35: 11000000 INFO @ Fri, 26 Jun 2020 09:00:35: 8000000 INFO @ Fri, 26 Jun 2020 09:00:41: 5000000 INFO @ Fri, 26 Jun 2020 09:00:43: 9000000 INFO @ Fri, 26 Jun 2020 09:00:43: 12000000 INFO @ Fri, 26 Jun 2020 09:00:49: 6000000 INFO @ Fri, 26 Jun 2020 09:00:50: 10000000 INFO @ Fri, 26 Jun 2020 09:00:52: 13000000 INFO @ Fri, 26 Jun 2020 09:00:57: 7000000 INFO @ Fri, 26 Jun 2020 09:00:57: 11000000 INFO @ Fri, 26 Jun 2020 09:01:00: 14000000 INFO @ Fri, 26 Jun 2020 09:01:04: 8000000 INFO @ Fri, 26 Jun 2020 09:01:05: 12000000 INFO @ Fri, 26 Jun 2020 09:01:08: 15000000 INFO @ Fri, 26 Jun 2020 09:01:11: 9000000 INFO @ Fri, 26 Jun 2020 09:01:12: 13000000 INFO @ Fri, 26 Jun 2020 09:01:16: 16000000 INFO @ Fri, 26 Jun 2020 09:01:18: 10000000 INFO @ Fri, 26 Jun 2020 09:01:20: 14000000 INFO @ Fri, 26 Jun 2020 09:01:24: 17000000 INFO @ Fri, 26 Jun 2020 09:01:25: 11000000 INFO @ Fri, 26 Jun 2020 09:01:27: 15000000 INFO @ Fri, 26 Jun 2020 09:01:32: 18000000 INFO @ Fri, 26 Jun 2020 09:01:33: 12000000 INFO @ Fri, 26 Jun 2020 09:01:35: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:01:39: 19000000 INFO @ Fri, 26 Jun 2020 09:01:39: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:01:39: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:01:39: #1 total tags in treatment: 19036117 INFO @ Fri, 26 Jun 2020 09:01:39: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:01:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:01:40: #1 tags after filtering in treatment: 19036117 INFO @ Fri, 26 Jun 2020 09:01:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:01:40: #1 finished! INFO @ Fri, 26 Jun 2020 09:01:40: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:01:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:01:40: 13000000 INFO @ Fri, 26 Jun 2020 09:01:41: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 09:01:41: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:01:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:01:43: 17000000 INFO @ Fri, 26 Jun 2020 09:01:47: 14000000 INFO @ Fri, 26 Jun 2020 09:01:50: 18000000 INFO @ Fri, 26 Jun 2020 09:01:54: 15000000 INFO @ Fri, 26 Jun 2020 09:01:57: 19000000 INFO @ Fri, 26 Jun 2020 09:01:57: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:01:57: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:01:57: #1 total tags in treatment: 19036117 INFO @ Fri, 26 Jun 2020 09:01:57: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:01:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:01:58: #1 tags after filtering in treatment: 19036117 INFO @ Fri, 26 Jun 2020 09:01:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:01:58: #1 finished! INFO @ Fri, 26 Jun 2020 09:01:58: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:01:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:01:59: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 09:01:59: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:01:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:02:01: 16000000 INFO @ Fri, 26 Jun 2020 09:02:08: 17000000 INFO @ Fri, 26 Jun 2020 09:02:15: 18000000 INFO @ Fri, 26 Jun 2020 09:02:21: 19000000 INFO @ Fri, 26 Jun 2020 09:02:22: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:02:22: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:02:22: #1 total tags in treatment: 19036117 INFO @ Fri, 26 Jun 2020 09:02:22: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:02:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:02:22: #1 tags after filtering in treatment: 19036117 INFO @ Fri, 26 Jun 2020 09:02:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:02:22: #1 finished! INFO @ Fri, 26 Jun 2020 09:02:22: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:02:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:02:23: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 09:02:23: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:02:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495225/SRX495225.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。