Job ID = 6498388 SRX = SRX495223 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T00:06:39 prefetch.2.10.7: 1) Downloading 'SRR1198728'... 2020-06-26T00:06:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:09:15 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:09:15 prefetch.2.10.7: 1) 'SRR1198728' was downloaded successfully Read 23604057 spots for SRR1198728/SRR1198728.sra Written 23604057 spots for SRR1198728/SRR1198728.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:05 23604057 reads; of these: 23604057 (100.00%) were unpaired; of these: 1733969 (7.35%) aligned 0 times 15645426 (66.28%) aligned exactly 1 time 6224662 (26.37%) aligned >1 times 92.65% overall alignment rate Time searching: 00:09:05 Overall time: 00:09:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5465338 / 21870088 = 0.2499 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:25:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:25:19: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:25:19: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:25:27: 1000000 INFO @ Fri, 26 Jun 2020 09:25:35: 2000000 INFO @ Fri, 26 Jun 2020 09:25:42: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:25:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:25:49: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:25:49: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:25:50: 4000000 INFO @ Fri, 26 Jun 2020 09:25:57: 1000000 INFO @ Fri, 26 Jun 2020 09:25:58: 5000000 INFO @ Fri, 26 Jun 2020 09:26:05: 2000000 INFO @ Fri, 26 Jun 2020 09:26:06: 6000000 INFO @ Fri, 26 Jun 2020 09:26:12: 3000000 INFO @ Fri, 26 Jun 2020 09:26:14: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:26:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:26:19: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:26:19: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:26:20: 4000000 INFO @ Fri, 26 Jun 2020 09:26:21: 8000000 INFO @ Fri, 26 Jun 2020 09:26:28: 1000000 INFO @ Fri, 26 Jun 2020 09:26:28: 5000000 INFO @ Fri, 26 Jun 2020 09:26:30: 9000000 INFO @ Fri, 26 Jun 2020 09:26:36: 2000000 INFO @ Fri, 26 Jun 2020 09:26:37: 6000000 INFO @ Fri, 26 Jun 2020 09:26:38: 10000000 INFO @ Fri, 26 Jun 2020 09:26:45: 7000000 INFO @ Fri, 26 Jun 2020 09:26:45: 3000000 INFO @ Fri, 26 Jun 2020 09:26:46: 11000000 INFO @ Fri, 26 Jun 2020 09:26:53: 8000000 INFO @ Fri, 26 Jun 2020 09:26:54: 12000000 INFO @ Fri, 26 Jun 2020 09:26:54: 4000000 INFO @ Fri, 26 Jun 2020 09:27:01: 9000000 INFO @ Fri, 26 Jun 2020 09:27:02: 13000000 INFO @ Fri, 26 Jun 2020 09:27:03: 5000000 INFO @ Fri, 26 Jun 2020 09:27:09: 10000000 INFO @ Fri, 26 Jun 2020 09:27:10: 14000000 INFO @ Fri, 26 Jun 2020 09:27:11: 6000000 INFO @ Fri, 26 Jun 2020 09:27:16: 11000000 INFO @ Fri, 26 Jun 2020 09:27:18: 15000000 INFO @ Fri, 26 Jun 2020 09:27:20: 7000000 INFO @ Fri, 26 Jun 2020 09:27:24: 12000000 INFO @ Fri, 26 Jun 2020 09:27:26: 16000000 INFO @ Fri, 26 Jun 2020 09:27:28: 8000000 INFO @ Fri, 26 Jun 2020 09:27:29: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:27:29: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:27:29: #1 total tags in treatment: 16404750 INFO @ Fri, 26 Jun 2020 09:27:29: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:27:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:27:29: #1 tags after filtering in treatment: 16404750 INFO @ Fri, 26 Jun 2020 09:27:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:27:29: #1 finished! INFO @ Fri, 26 Jun 2020 09:27:29: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:27:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:27:31: #2 number of paired peaks: 15 WARNING @ Fri, 26 Jun 2020 09:27:31: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:27:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:27:32: 13000000 INFO @ Fri, 26 Jun 2020 09:27:37: 9000000 INFO @ Fri, 26 Jun 2020 09:27:40: 14000000 INFO @ Fri, 26 Jun 2020 09:27:45: 10000000 INFO @ Fri, 26 Jun 2020 09:27:48: 15000000 INFO @ Fri, 26 Jun 2020 09:27:53: 11000000 INFO @ Fri, 26 Jun 2020 09:27:55: 16000000 INFO @ Fri, 26 Jun 2020 09:27:59: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:27:59: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:27:59: #1 total tags in treatment: 16404750 INFO @ Fri, 26 Jun 2020 09:27:59: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:27:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:27:59: #1 tags after filtering in treatment: 16404750 INFO @ Fri, 26 Jun 2020 09:27:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:27:59: #1 finished! INFO @ Fri, 26 Jun 2020 09:27:59: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:27:59: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:28:00: #2 number of paired peaks: 15 WARNING @ Fri, 26 Jun 2020 09:28:00: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:28:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:28:01: 12000000 INFO @ Fri, 26 Jun 2020 09:28:09: 13000000 INFO @ Fri, 26 Jun 2020 09:28:17: 14000000 INFO @ Fri, 26 Jun 2020 09:28:24: 15000000 INFO @ Fri, 26 Jun 2020 09:28:32: 16000000 INFO @ Fri, 26 Jun 2020 09:28:35: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:28:35: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:28:35: #1 total tags in treatment: 16404750 INFO @ Fri, 26 Jun 2020 09:28:35: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:28:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:28:36: #1 tags after filtering in treatment: 16404750 INFO @ Fri, 26 Jun 2020 09:28:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:28:36: #1 finished! INFO @ Fri, 26 Jun 2020 09:28:36: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:28:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:28:37: #2 number of paired peaks: 15 WARNING @ Fri, 26 Jun 2020 09:28:37: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:28:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495223/SRX495223.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。