Job ID = 6498386 SRX = SRX495221 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:36:45 prefetch.2.10.7: 1) Downloading 'SRR1198726'... 2020-06-25T23:36:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:39:41 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:39:41 prefetch.2.10.7: 1) 'SRR1198726' was downloaded successfully Read 18189117 spots for SRR1198726/SRR1198726.sra Written 18189117 spots for SRR1198726/SRR1198726.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:11 18189117 reads; of these: 18189117 (100.00%) were unpaired; of these: 703130 (3.87%) aligned 0 times 12502428 (68.74%) aligned exactly 1 time 4983559 (27.40%) aligned >1 times 96.13% overall alignment rate Time searching: 00:06:12 Overall time: 00:06:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3084650 / 17485987 = 0.1764 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:51:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:51:46: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:51:46: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:51:51: 1000000 INFO @ Fri, 26 Jun 2020 08:51:57: 2000000 INFO @ Fri, 26 Jun 2020 08:52:02: 3000000 INFO @ Fri, 26 Jun 2020 08:52:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:52:13: 5000000 INFO @ Fri, 26 Jun 2020 08:52:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:52:15: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:52:15: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:52:19: 6000000 INFO @ Fri, 26 Jun 2020 08:52:21: 1000000 INFO @ Fri, 26 Jun 2020 08:52:26: 7000000 INFO @ Fri, 26 Jun 2020 08:52:27: 2000000 INFO @ Fri, 26 Jun 2020 08:52:32: 8000000 INFO @ Fri, 26 Jun 2020 08:52:32: 3000000 INFO @ Fri, 26 Jun 2020 08:52:38: 9000000 INFO @ Fri, 26 Jun 2020 08:52:38: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:52:44: 5000000 INFO @ Fri, 26 Jun 2020 08:52:44: 10000000 INFO @ Fri, 26 Jun 2020 08:52:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:52:45: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:52:45: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:52:50: 6000000 INFO @ Fri, 26 Jun 2020 08:52:51: 11000000 INFO @ Fri, 26 Jun 2020 08:52:51: 1000000 INFO @ Fri, 26 Jun 2020 08:52:56: 7000000 INFO @ Fri, 26 Jun 2020 08:52:57: 2000000 INFO @ Fri, 26 Jun 2020 08:52:58: 12000000 INFO @ Fri, 26 Jun 2020 08:53:02: 8000000 INFO @ Fri, 26 Jun 2020 08:53:03: 3000000 INFO @ Fri, 26 Jun 2020 08:53:04: 13000000 INFO @ Fri, 26 Jun 2020 08:53:08: 9000000 INFO @ Fri, 26 Jun 2020 08:53:09: 4000000 INFO @ Fri, 26 Jun 2020 08:53:10: 14000000 INFO @ Fri, 26 Jun 2020 08:53:13: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:53:13: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:53:13: #1 total tags in treatment: 14401337 INFO @ Fri, 26 Jun 2020 08:53:13: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:53:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:53:13: #1 tags after filtering in treatment: 14401337 INFO @ Fri, 26 Jun 2020 08:53:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:53:13: #1 finished! INFO @ Fri, 26 Jun 2020 08:53:13: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:53:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:53:14: 10000000 INFO @ Fri, 26 Jun 2020 08:53:14: #2 number of paired peaks: 40 WARNING @ Fri, 26 Jun 2020 08:53:14: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:53:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:53:15: 5000000 INFO @ Fri, 26 Jun 2020 08:53:20: 11000000 INFO @ Fri, 26 Jun 2020 08:53:21: 6000000 INFO @ Fri, 26 Jun 2020 08:53:26: 12000000 INFO @ Fri, 26 Jun 2020 08:53:27: 7000000 INFO @ Fri, 26 Jun 2020 08:53:32: 13000000 INFO @ Fri, 26 Jun 2020 08:53:33: 8000000 INFO @ Fri, 26 Jun 2020 08:53:38: 14000000 INFO @ Fri, 26 Jun 2020 08:53:39: 9000000 INFO @ Fri, 26 Jun 2020 08:53:41: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:53:41: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:53:41: #1 total tags in treatment: 14401337 INFO @ Fri, 26 Jun 2020 08:53:41: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:53:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:53:41: #1 tags after filtering in treatment: 14401337 INFO @ Fri, 26 Jun 2020 08:53:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:53:41: #1 finished! INFO @ Fri, 26 Jun 2020 08:53:41: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:53:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:53:42: #2 number of paired peaks: 40 WARNING @ Fri, 26 Jun 2020 08:53:42: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:53:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:53:45: 10000000 INFO @ Fri, 26 Jun 2020 08:53:51: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:53:57: 12000000 INFO @ Fri, 26 Jun 2020 08:54:03: 13000000 INFO @ Fri, 26 Jun 2020 08:54:09: 14000000 INFO @ Fri, 26 Jun 2020 08:54:11: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:54:11: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:54:11: #1 total tags in treatment: 14401337 INFO @ Fri, 26 Jun 2020 08:54:11: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:54:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:54:11: #1 tags after filtering in treatment: 14401337 INFO @ Fri, 26 Jun 2020 08:54:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:54:11: #1 finished! INFO @ Fri, 26 Jun 2020 08:54:11: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:54:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:54:12: #2 number of paired peaks: 40 WARNING @ Fri, 26 Jun 2020 08:54:12: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:54:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495221/SRX495221.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。