Job ID = 6528262 SRX = SRX495218 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:48:13 prefetch.2.10.7: 1) Downloading 'SRR1198723'... 2020-06-29T14:48:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:49:54 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:49:55 prefetch.2.10.7: 'SRR1198723' is valid 2020-06-29T14:49:55 prefetch.2.10.7: 1) 'SRR1198723' was downloaded successfully Read 17232507 spots for SRR1198723/SRR1198723.sra Written 17232507 spots for SRR1198723/SRR1198723.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:41 17232507 reads; of these: 17232507 (100.00%) were unpaired; of these: 207612 (1.20%) aligned 0 times 12449011 (72.24%) aligned exactly 1 time 4575884 (26.55%) aligned >1 times 98.80% overall alignment rate Time searching: 00:05:41 Overall time: 00:05:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3298147 / 17024895 = 0.1937 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:06:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:06:46: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:06:46: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:06:51: 1000000 INFO @ Tue, 30 Jun 2020 00:06:56: 2000000 INFO @ Tue, 30 Jun 2020 00:07:01: 3000000 INFO @ Tue, 30 Jun 2020 00:07:05: 4000000 INFO @ Tue, 30 Jun 2020 00:07:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:15: 6000000 INFO @ Tue, 30 Jun 2020 00:07:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:20: 7000000 INFO @ Tue, 30 Jun 2020 00:07:21: 1000000 INFO @ Tue, 30 Jun 2020 00:07:26: 8000000 INFO @ Tue, 30 Jun 2020 00:07:27: 2000000 INFO @ Tue, 30 Jun 2020 00:07:31: 9000000 INFO @ Tue, 30 Jun 2020 00:07:32: 3000000 INFO @ Tue, 30 Jun 2020 00:07:36: 10000000 INFO @ Tue, 30 Jun 2020 00:07:37: 4000000 INFO @ Tue, 30 Jun 2020 00:07:42: 11000000 INFO @ Tue, 30 Jun 2020 00:07:42: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:46: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:46: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:47: 12000000 INFO @ Tue, 30 Jun 2020 00:07:48: 6000000 INFO @ Tue, 30 Jun 2020 00:07:52: 1000000 INFO @ Tue, 30 Jun 2020 00:07:53: 13000000 INFO @ Tue, 30 Jun 2020 00:07:53: 7000000 INFO @ Tue, 30 Jun 2020 00:07:57: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:07:57: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:07:57: #1 total tags in treatment: 13726748 INFO @ Tue, 30 Jun 2020 00:07:57: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:07:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:07:57: #1 tags after filtering in treatment: 13726748 INFO @ Tue, 30 Jun 2020 00:07:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:07:57: #1 finished! INFO @ Tue, 30 Jun 2020 00:07:57: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:07:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:07:58: 2000000 INFO @ Tue, 30 Jun 2020 00:07:58: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:07:58: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:07:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:07:59: 8000000 INFO @ Tue, 30 Jun 2020 00:08:03: 3000000 INFO @ Tue, 30 Jun 2020 00:08:04: 9000000 INFO @ Tue, 30 Jun 2020 00:08:08: 4000000 INFO @ Tue, 30 Jun 2020 00:08:09: 10000000 INFO @ Tue, 30 Jun 2020 00:08:13: 5000000 INFO @ Tue, 30 Jun 2020 00:08:14: 11000000 INFO @ Tue, 30 Jun 2020 00:08:18: 6000000 INFO @ Tue, 30 Jun 2020 00:08:20: 12000000 INFO @ Tue, 30 Jun 2020 00:08:23: 7000000 INFO @ Tue, 30 Jun 2020 00:08:25: 13000000 INFO @ Tue, 30 Jun 2020 00:08:29: 8000000 INFO @ Tue, 30 Jun 2020 00:08:29: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:08:29: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:08:29: #1 total tags in treatment: 13726748 INFO @ Tue, 30 Jun 2020 00:08:29: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:08:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:08:29: #1 tags after filtering in treatment: 13726748 INFO @ Tue, 30 Jun 2020 00:08:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:08:29: #1 finished! INFO @ Tue, 30 Jun 2020 00:08:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:08:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:08:30: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:08:30: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:08:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:08:33: 9000000 INFO @ Tue, 30 Jun 2020 00:08:38: 10000000 INFO @ Tue, 30 Jun 2020 00:08:43: 11000000 INFO @ Tue, 30 Jun 2020 00:08:48: 12000000 INFO @ Tue, 30 Jun 2020 00:08:52: 13000000 INFO @ Tue, 30 Jun 2020 00:08:56: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:08:56: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:08:56: #1 total tags in treatment: 13726748 INFO @ Tue, 30 Jun 2020 00:08:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:08:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:08:56: #1 tags after filtering in treatment: 13726748 INFO @ Tue, 30 Jun 2020 00:08:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:08:56: #1 finished! INFO @ Tue, 30 Jun 2020 00:08:56: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:08:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:08:57: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:08:57: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:08:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495218/SRX495218.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。