Job ID = 6528260 SRX = SRX495215 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:39:28 prefetch.2.10.7: 1) Downloading 'SRR1198720'... 2020-06-29T14:39:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:41:46 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:41:46 prefetch.2.10.7: 1) 'SRR1198720' was downloaded successfully Read 20834747 spots for SRR1198720/SRR1198720.sra Written 20834747 spots for SRR1198720/SRR1198720.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:00 20834747 reads; of these: 20834747 (100.00%) were unpaired; of these: 3183106 (15.28%) aligned 0 times 13863320 (66.54%) aligned exactly 1 time 3788321 (18.18%) aligned >1 times 84.72% overall alignment rate Time searching: 00:05:00 Overall time: 00:05:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3424335 / 17651641 = 0.1940 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:31: 1000000 INFO @ Mon, 29 Jun 2020 23:56:36: 2000000 INFO @ Mon, 29 Jun 2020 23:56:42: 3000000 INFO @ Mon, 29 Jun 2020 23:56:47: 4000000 INFO @ Mon, 29 Jun 2020 23:56:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:55: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:55: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:58: 6000000 INFO @ Mon, 29 Jun 2020 23:57:02: 1000000 INFO @ Mon, 29 Jun 2020 23:57:04: 7000000 INFO @ Mon, 29 Jun 2020 23:57:09: 2000000 INFO @ Mon, 29 Jun 2020 23:57:10: 8000000 INFO @ Mon, 29 Jun 2020 23:57:16: 9000000 INFO @ Mon, 29 Jun 2020 23:57:17: 3000000 INFO @ Mon, 29 Jun 2020 23:57:23: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:57:24: 4000000 INFO @ Mon, 29 Jun 2020 23:57:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:57:25: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:57:25: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:57:29: 11000000 INFO @ Mon, 29 Jun 2020 23:57:31: 5000000 INFO @ Mon, 29 Jun 2020 23:57:32: 1000000 INFO @ Mon, 29 Jun 2020 23:57:35: 12000000 INFO @ Mon, 29 Jun 2020 23:57:39: 6000000 INFO @ Mon, 29 Jun 2020 23:57:39: 2000000 INFO @ Mon, 29 Jun 2020 23:57:42: 13000000 INFO @ Mon, 29 Jun 2020 23:57:46: 7000000 INFO @ Mon, 29 Jun 2020 23:57:46: 3000000 INFO @ Mon, 29 Jun 2020 23:57:48: 14000000 INFO @ Mon, 29 Jun 2020 23:57:50: #1 tag size is determined as 44 bps INFO @ Mon, 29 Jun 2020 23:57:50: #1 tag size = 44 INFO @ Mon, 29 Jun 2020 23:57:50: #1 total tags in treatment: 14227306 INFO @ Mon, 29 Jun 2020 23:57:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:57:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:57:50: #1 tags after filtering in treatment: 14227306 INFO @ Mon, 29 Jun 2020 23:57:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:57:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:57:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:57:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:57:51: #2 number of paired peaks: 21 WARNING @ Mon, 29 Jun 2020 23:57:51: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:57:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:57:53: 4000000 INFO @ Mon, 29 Jun 2020 23:57:53: 8000000 INFO @ Mon, 29 Jun 2020 23:58:00: 5000000 INFO @ Mon, 29 Jun 2020 23:58:00: 9000000 INFO @ Mon, 29 Jun 2020 23:58:06: 6000000 INFO @ Mon, 29 Jun 2020 23:58:08: 10000000 INFO @ Mon, 29 Jun 2020 23:58:13: 7000000 INFO @ Mon, 29 Jun 2020 23:58:15: 11000000 INFO @ Mon, 29 Jun 2020 23:58:20: 8000000 INFO @ Mon, 29 Jun 2020 23:58:22: 12000000 INFO @ Mon, 29 Jun 2020 23:58:27: 9000000 INFO @ Mon, 29 Jun 2020 23:58:30: 13000000 INFO @ Mon, 29 Jun 2020 23:58:34: 10000000 INFO @ Mon, 29 Jun 2020 23:58:37: 14000000 INFO @ Mon, 29 Jun 2020 23:58:38: #1 tag size is determined as 44 bps INFO @ Mon, 29 Jun 2020 23:58:38: #1 tag size = 44 INFO @ Mon, 29 Jun 2020 23:58:38: #1 total tags in treatment: 14227306 INFO @ Mon, 29 Jun 2020 23:58:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:58:39: #1 tags after filtering in treatment: 14227306 INFO @ Mon, 29 Jun 2020 23:58:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:58:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:58:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:58:39: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:58:40: #2 number of paired peaks: 21 WARNING @ Mon, 29 Jun 2020 23:58:40: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:58:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:58:41: 11000000 INFO @ Mon, 29 Jun 2020 23:58:47: 12000000 INFO @ Mon, 29 Jun 2020 23:58:54: 13000000 INFO @ Mon, 29 Jun 2020 23:59:00: 14000000 INFO @ Mon, 29 Jun 2020 23:59:02: #1 tag size is determined as 44 bps INFO @ Mon, 29 Jun 2020 23:59:02: #1 tag size = 44 INFO @ Mon, 29 Jun 2020 23:59:02: #1 total tags in treatment: 14227306 INFO @ Mon, 29 Jun 2020 23:59:02: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:02: #1 tags after filtering in treatment: 14227306 INFO @ Mon, 29 Jun 2020 23:59:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:02: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:02: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:03: #2 number of paired peaks: 21 WARNING @ Mon, 29 Jun 2020 23:59:03: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495215/SRX495215.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。