Job ID = 6498379 SRX = SRX495214 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:25:17 prefetch.2.10.7: 1) Downloading 'SRR1198719'... 2020-06-25T23:25:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:27:43 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:27:43 prefetch.2.10.7: 1) 'SRR1198719' was downloaded successfully Read 23630901 spots for SRR1198719/SRR1198719.sra Written 23630901 spots for SRR1198719/SRR1198719.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:28 23630901 reads; of these: 23630901 (100.00%) were unpaired; of these: 563333 (2.38%) aligned 0 times 18499585 (78.29%) aligned exactly 1 time 4567983 (19.33%) aligned >1 times 97.62% overall alignment rate Time searching: 00:07:28 Overall time: 00:07:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4554960 / 23067568 = 0.1975 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:42:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:42:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:42:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:42:45: 1000000 INFO @ Fri, 26 Jun 2020 08:42:53: 2000000 INFO @ Fri, 26 Jun 2020 08:43:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:43:07: 4000000 INFO @ Fri, 26 Jun 2020 08:43:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:43:08: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:43:08: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:43:15: 5000000 INFO @ Fri, 26 Jun 2020 08:43:16: 1000000 INFO @ Fri, 26 Jun 2020 08:43:24: 2000000 INFO @ Fri, 26 Jun 2020 08:43:24: 6000000 INFO @ Fri, 26 Jun 2020 08:43:32: 3000000 INFO @ Fri, 26 Jun 2020 08:43:32: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:43:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:43:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:43:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:43:40: 4000000 INFO @ Fri, 26 Jun 2020 08:43:40: 8000000 INFO @ Fri, 26 Jun 2020 08:43:46: 1000000 INFO @ Fri, 26 Jun 2020 08:43:48: 5000000 INFO @ Fri, 26 Jun 2020 08:43:49: 9000000 INFO @ Fri, 26 Jun 2020 08:43:54: 2000000 INFO @ Fri, 26 Jun 2020 08:43:56: 6000000 INFO @ Fri, 26 Jun 2020 08:43:57: 10000000 INFO @ Fri, 26 Jun 2020 08:44:02: 3000000 INFO @ Fri, 26 Jun 2020 08:44:04: 7000000 INFO @ Fri, 26 Jun 2020 08:44:06: 11000000 INFO @ Fri, 26 Jun 2020 08:44:10: 4000000 INFO @ Fri, 26 Jun 2020 08:44:12: 8000000 INFO @ Fri, 26 Jun 2020 08:44:14: 12000000 INFO @ Fri, 26 Jun 2020 08:44:18: 5000000 INFO @ Fri, 26 Jun 2020 08:44:20: 9000000 INFO @ Fri, 26 Jun 2020 08:44:23: 13000000 INFO @ Fri, 26 Jun 2020 08:44:26: 6000000 INFO @ Fri, 26 Jun 2020 08:44:28: 10000000 INFO @ Fri, 26 Jun 2020 08:44:31: 14000000 INFO @ Fri, 26 Jun 2020 08:44:34: 7000000 INFO @ Fri, 26 Jun 2020 08:44:36: 11000000 INFO @ Fri, 26 Jun 2020 08:44:40: 15000000 INFO @ Fri, 26 Jun 2020 08:44:42: 8000000 INFO @ Fri, 26 Jun 2020 08:44:44: 12000000 INFO @ Fri, 26 Jun 2020 08:44:48: 16000000 INFO @ Fri, 26 Jun 2020 08:44:50: 9000000 INFO @ Fri, 26 Jun 2020 08:44:52: 13000000 INFO @ Fri, 26 Jun 2020 08:44:57: 17000000 INFO @ Fri, 26 Jun 2020 08:44:58: 10000000 INFO @ Fri, 26 Jun 2020 08:45:00: 14000000 INFO @ Fri, 26 Jun 2020 08:45:05: 18000000 INFO @ Fri, 26 Jun 2020 08:45:06: 11000000 INFO @ Fri, 26 Jun 2020 08:45:08: 15000000 INFO @ Fri, 26 Jun 2020 08:45:10: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:45:10: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:45:10: #1 total tags in treatment: 18512608 INFO @ Fri, 26 Jun 2020 08:45:10: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:45:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:45:10: #1 tags after filtering in treatment: 18512608 INFO @ Fri, 26 Jun 2020 08:45:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:45:10: #1 finished! INFO @ Fri, 26 Jun 2020 08:45:10: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:45:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:45:12: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 08:45:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:45:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:45:13: 12000000 INFO @ Fri, 26 Jun 2020 08:45:15: 16000000 INFO @ Fri, 26 Jun 2020 08:45:21: 13000000 INFO @ Fri, 26 Jun 2020 08:45:22: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:45:28: 14000000 INFO @ Fri, 26 Jun 2020 08:45:30: 18000000 INFO @ Fri, 26 Jun 2020 08:45:34: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:45:34: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:45:34: #1 total tags in treatment: 18512608 INFO @ Fri, 26 Jun 2020 08:45:34: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:45:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:45:34: #1 tags after filtering in treatment: 18512608 INFO @ Fri, 26 Jun 2020 08:45:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:45:34: #1 finished! INFO @ Fri, 26 Jun 2020 08:45:34: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:45:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:45:35: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 08:45:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:45:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:45:36: 15000000 INFO @ Fri, 26 Jun 2020 08:45:42: 16000000 INFO @ Fri, 26 Jun 2020 08:45:49: 17000000 INFO @ Fri, 26 Jun 2020 08:45:56: 18000000 INFO @ Fri, 26 Jun 2020 08:45:59: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:45:59: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:45:59: #1 total tags in treatment: 18512608 INFO @ Fri, 26 Jun 2020 08:45:59: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:45:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:45:59: #1 tags after filtering in treatment: 18512608 INFO @ Fri, 26 Jun 2020 08:45:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:45:59: #1 finished! INFO @ Fri, 26 Jun 2020 08:45:59: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:45:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:46:01: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 08:46:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:46:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495214/SRX495214.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。