Job ID = 1304858 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,251,934 reads read : 22,251,934 reads written : 22,251,934 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:22 22251934 reads; of these: 22251934 (100.00%) were unpaired; of these: 832617 (3.74%) aligned 0 times 15008478 (67.45%) aligned exactly 1 time 6410839 (28.81%) aligned >1 times 96.26% overall alignment rate Time searching: 00:08:22 Overall time: 00:08:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5187528 / 21419317 = 0.2422 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:12:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:12:52: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:12:52: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:12:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:12:52: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:12:52: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:12:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:12:52: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:12:52: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:12:59: 1000000 INFO @ Mon, 03 Jun 2019 21:13:00: 1000000 INFO @ Mon, 03 Jun 2019 21:13:02: 1000000 INFO @ Mon, 03 Jun 2019 21:13:06: 2000000 INFO @ Mon, 03 Jun 2019 21:13:08: 2000000 INFO @ Mon, 03 Jun 2019 21:13:10: 2000000 INFO @ Mon, 03 Jun 2019 21:13:13: 3000000 INFO @ Mon, 03 Jun 2019 21:13:15: 3000000 INFO @ Mon, 03 Jun 2019 21:13:18: 3000000 INFO @ Mon, 03 Jun 2019 21:13:19: 4000000 INFO @ Mon, 03 Jun 2019 21:13:23: 4000000 INFO @ Mon, 03 Jun 2019 21:13:26: 5000000 INFO @ Mon, 03 Jun 2019 21:13:27: 4000000 INFO @ Mon, 03 Jun 2019 21:13:30: 5000000 INFO @ Mon, 03 Jun 2019 21:13:32: 6000000 INFO @ Mon, 03 Jun 2019 21:13:35: 5000000 INFO @ Mon, 03 Jun 2019 21:13:38: 6000000 INFO @ Mon, 03 Jun 2019 21:13:39: 7000000 INFO @ Mon, 03 Jun 2019 21:13:43: 6000000 INFO @ Mon, 03 Jun 2019 21:13:45: 7000000 INFO @ Mon, 03 Jun 2019 21:13:45: 8000000 INFO @ Mon, 03 Jun 2019 21:13:51: 7000000 INFO @ Mon, 03 Jun 2019 21:13:52: 9000000 INFO @ Mon, 03 Jun 2019 21:13:53: 8000000 INFO @ Mon, 03 Jun 2019 21:13:59: 10000000 INFO @ Mon, 03 Jun 2019 21:14:00: 8000000 INFO @ Mon, 03 Jun 2019 21:14:00: 9000000 INFO @ Mon, 03 Jun 2019 21:14:05: 11000000 INFO @ Mon, 03 Jun 2019 21:14:07: 10000000 INFO @ Mon, 03 Jun 2019 21:14:08: 9000000 INFO @ Mon, 03 Jun 2019 21:14:12: 12000000 INFO @ Mon, 03 Jun 2019 21:14:15: 11000000 INFO @ Mon, 03 Jun 2019 21:14:16: 10000000 INFO @ Mon, 03 Jun 2019 21:14:18: 13000000 INFO @ Mon, 03 Jun 2019 21:14:22: 12000000 INFO @ Mon, 03 Jun 2019 21:14:25: 11000000 INFO @ Mon, 03 Jun 2019 21:14:25: 14000000 INFO @ Mon, 03 Jun 2019 21:14:29: 13000000 INFO @ Mon, 03 Jun 2019 21:14:32: 15000000 INFO @ Mon, 03 Jun 2019 21:14:33: 12000000 INFO @ Mon, 03 Jun 2019 21:14:36: 14000000 INFO @ Mon, 03 Jun 2019 21:14:40: 16000000 INFO @ Mon, 03 Jun 2019 21:14:41: 13000000 INFO @ Mon, 03 Jun 2019 21:14:41: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:14:41: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:14:41: #1 total tags in treatment: 16231789 INFO @ Mon, 03 Jun 2019 21:14:41: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:14:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:14:42: #1 tags after filtering in treatment: 16231789 INFO @ Mon, 03 Jun 2019 21:14:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:14:42: #1 finished! INFO @ Mon, 03 Jun 2019 21:14:42: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:14:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:14:42: 15000000 INFO @ Mon, 03 Jun 2019 21:14:43: #2 number of paired peaks: 3 WARNING @ Mon, 03 Jun 2019 21:14:43: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:14:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:14:49: 16000000 INFO @ Mon, 03 Jun 2019 21:14:49: 14000000 INFO @ Mon, 03 Jun 2019 21:14:50: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:14:50: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:14:50: #1 total tags in treatment: 16231789 INFO @ Mon, 03 Jun 2019 21:14:50: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:14:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:14:51: #1 tags after filtering in treatment: 16231789 INFO @ Mon, 03 Jun 2019 21:14:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:14:51: #1 finished! INFO @ Mon, 03 Jun 2019 21:14:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:14:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:14:52: #2 number of paired peaks: 3 WARNING @ Mon, 03 Jun 2019 21:14:52: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:14:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:14:57: 15000000 INFO @ Mon, 03 Jun 2019 21:15:04: 16000000 INFO @ Mon, 03 Jun 2019 21:15:06: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:15:06: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:15:06: #1 total tags in treatment: 16231789 INFO @ Mon, 03 Jun 2019 21:15:06: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:15:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:15:06: #1 tags after filtering in treatment: 16231789 INFO @ Mon, 03 Jun 2019 21:15:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:15:06: #1 finished! INFO @ Mon, 03 Jun 2019 21:15:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:15:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:15:08: #2 number of paired peaks: 3 WARNING @ Mon, 03 Jun 2019 21:15:08: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:15:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495208/SRX495208.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。