Job ID = 6498367 SRX = SRX495194 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:37:15 prefetch.2.10.7: 1) Downloading 'SRR1198699'... 2020-06-25T23:37:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:39:24 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:39:24 prefetch.2.10.7: 1) 'SRR1198699' was downloaded successfully Read 21914988 spots for SRR1198699/SRR1198699.sra Written 21914988 spots for SRR1198699/SRR1198699.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:57 21914988 reads; of these: 21914988 (100.00%) were unpaired; of these: 2148737 (9.80%) aligned 0 times 17615148 (80.38%) aligned exactly 1 time 2151103 (9.82%) aligned >1 times 90.20% overall alignment rate Time searching: 00:04:57 Overall time: 00:04:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3543257 / 19766251 = 0.1793 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:50:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:50:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:50:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:50:25: 1000000 INFO @ Fri, 26 Jun 2020 08:50:31: 2000000 INFO @ Fri, 26 Jun 2020 08:50:38: 3000000 INFO @ Fri, 26 Jun 2020 08:50:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:50:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:50:48: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:50:48: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:50:51: 5000000 INFO @ Fri, 26 Jun 2020 08:50:55: 1000000 INFO @ Fri, 26 Jun 2020 08:50:58: 6000000 INFO @ Fri, 26 Jun 2020 08:51:02: 2000000 INFO @ Fri, 26 Jun 2020 08:51:05: 7000000 INFO @ Fri, 26 Jun 2020 08:51:10: 3000000 INFO @ Fri, 26 Jun 2020 08:51:13: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:51:17: 4000000 INFO @ Fri, 26 Jun 2020 08:51:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:51:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:51:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:51:20: 9000000 INFO @ Fri, 26 Jun 2020 08:51:24: 5000000 INFO @ Fri, 26 Jun 2020 08:51:26: 1000000 INFO @ Fri, 26 Jun 2020 08:51:27: 10000000 INFO @ Fri, 26 Jun 2020 08:51:31: 6000000 INFO @ Fri, 26 Jun 2020 08:51:33: 2000000 INFO @ Fri, 26 Jun 2020 08:51:35: 11000000 INFO @ Fri, 26 Jun 2020 08:51:39: 7000000 INFO @ Fri, 26 Jun 2020 08:51:41: 3000000 INFO @ Fri, 26 Jun 2020 08:51:42: 12000000 INFO @ Fri, 26 Jun 2020 08:51:46: 8000000 INFO @ Fri, 26 Jun 2020 08:51:49: 4000000 INFO @ Fri, 26 Jun 2020 08:51:49: 13000000 INFO @ Fri, 26 Jun 2020 08:51:53: 9000000 INFO @ Fri, 26 Jun 2020 08:51:56: 5000000 INFO @ Fri, 26 Jun 2020 08:51:57: 14000000 INFO @ Fri, 26 Jun 2020 08:52:00: 10000000 INFO @ Fri, 26 Jun 2020 08:52:04: 6000000 INFO @ Fri, 26 Jun 2020 08:52:04: 15000000 INFO @ Fri, 26 Jun 2020 08:52:07: 11000000 INFO @ Fri, 26 Jun 2020 08:52:11: 7000000 INFO @ Fri, 26 Jun 2020 08:52:11: 16000000 INFO @ Fri, 26 Jun 2020 08:52:13: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:52:13: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:52:13: #1 total tags in treatment: 16222994 INFO @ Fri, 26 Jun 2020 08:52:13: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:52:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:52:13: #1 tags after filtering in treatment: 16222994 INFO @ Fri, 26 Jun 2020 08:52:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:52:13: #1 finished! INFO @ Fri, 26 Jun 2020 08:52:13: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:52:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:52:14: 12000000 INFO @ Fri, 26 Jun 2020 08:52:14: #2 number of paired peaks: 18 WARNING @ Fri, 26 Jun 2020 08:52:14: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:52:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:52:19: 8000000 INFO @ Fri, 26 Jun 2020 08:52:21: 13000000 INFO @ Fri, 26 Jun 2020 08:52:26: 9000000 INFO @ Fri, 26 Jun 2020 08:52:29: 14000000 INFO @ Fri, 26 Jun 2020 08:52:33: 10000000 INFO @ Fri, 26 Jun 2020 08:52:36: 15000000 INFO @ Fri, 26 Jun 2020 08:52:41: 11000000 INFO @ Fri, 26 Jun 2020 08:52:43: 16000000 INFO @ Fri, 26 Jun 2020 08:52:45: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:52:45: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:52:45: #1 total tags in treatment: 16222994 INFO @ Fri, 26 Jun 2020 08:52:45: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:52:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:52:45: #1 tags after filtering in treatment: 16222994 INFO @ Fri, 26 Jun 2020 08:52:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:52:45: #1 finished! INFO @ Fri, 26 Jun 2020 08:52:45: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:52:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:52:46: #2 number of paired peaks: 18 WARNING @ Fri, 26 Jun 2020 08:52:46: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:52:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:52:48: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:52:54: 13000000 INFO @ Fri, 26 Jun 2020 08:53:01: 14000000 INFO @ Fri, 26 Jun 2020 08:53:08: 15000000 INFO @ Fri, 26 Jun 2020 08:53:15: 16000000 INFO @ Fri, 26 Jun 2020 08:53:17: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:53:17: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:53:17: #1 total tags in treatment: 16222994 INFO @ Fri, 26 Jun 2020 08:53:17: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:53:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:53:17: #1 tags after filtering in treatment: 16222994 INFO @ Fri, 26 Jun 2020 08:53:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:53:17: #1 finished! INFO @ Fri, 26 Jun 2020 08:53:17: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:53:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:53:18: #2 number of paired peaks: 18 WARNING @ Fri, 26 Jun 2020 08:53:18: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:53:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495194/SRX495194.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。