Job ID = 2590615 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,914,988 reads read : 21,914,988 reads written : 21,914,988 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198698.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:17 21914988 reads; of these: 21914988 (100.00%) were unpaired; of these: 2148761 (9.80%) aligned 0 times 17615075 (80.38%) aligned exactly 1 time 2151152 (9.82%) aligned >1 times 90.20% overall alignment rate Time searching: 00:05:17 Overall time: 00:05:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3543000 / 19766227 = 0.1792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:50:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:50:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:50:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:50:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:50:33: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:50:33: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:50:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:50:34: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:50:34: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:50:40: 1000000 INFO @ Mon, 12 Aug 2019 22:50:41: 1000000 INFO @ Mon, 12 Aug 2019 22:50:42: 1000000 INFO @ Mon, 12 Aug 2019 22:50:48: 2000000 INFO @ Mon, 12 Aug 2019 22:50:50: 2000000 INFO @ Mon, 12 Aug 2019 22:50:50: 2000000 INFO @ Mon, 12 Aug 2019 22:50:56: 3000000 INFO @ Mon, 12 Aug 2019 22:50:58: 3000000 INFO @ Mon, 12 Aug 2019 22:50:59: 3000000 INFO @ Mon, 12 Aug 2019 22:51:04: 4000000 INFO @ Mon, 12 Aug 2019 22:51:06: 4000000 INFO @ Mon, 12 Aug 2019 22:51:08: 4000000 INFO @ Mon, 12 Aug 2019 22:51:11: 5000000 INFO @ Mon, 12 Aug 2019 22:51:14: 5000000 INFO @ Mon, 12 Aug 2019 22:51:17: 5000000 INFO @ Mon, 12 Aug 2019 22:51:19: 6000000 INFO @ Mon, 12 Aug 2019 22:51:22: 6000000 INFO @ Mon, 12 Aug 2019 22:51:26: 6000000 INFO @ Mon, 12 Aug 2019 22:51:27: 7000000 INFO @ Mon, 12 Aug 2019 22:51:30: 7000000 INFO @ Mon, 12 Aug 2019 22:51:35: 8000000 INFO @ Mon, 12 Aug 2019 22:51:35: 7000000 INFO @ Mon, 12 Aug 2019 22:51:37: 8000000 INFO @ Mon, 12 Aug 2019 22:51:43: 9000000 INFO @ Mon, 12 Aug 2019 22:51:44: 8000000 INFO @ Mon, 12 Aug 2019 22:51:45: 9000000 INFO @ Mon, 12 Aug 2019 22:51:51: 10000000 INFO @ Mon, 12 Aug 2019 22:51:53: 10000000 INFO @ Mon, 12 Aug 2019 22:51:53: 9000000 INFO @ Mon, 12 Aug 2019 22:51:59: 11000000 INFO @ Mon, 12 Aug 2019 22:52:01: 11000000 INFO @ Mon, 12 Aug 2019 22:52:02: 10000000 INFO @ Mon, 12 Aug 2019 22:52:06: 12000000 INFO @ Mon, 12 Aug 2019 22:52:09: 12000000 INFO @ Mon, 12 Aug 2019 22:52:11: 11000000 INFO @ Mon, 12 Aug 2019 22:52:14: 13000000 INFO @ Mon, 12 Aug 2019 22:52:17: 13000000 INFO @ Mon, 12 Aug 2019 22:52:20: 12000000 INFO @ Mon, 12 Aug 2019 22:52:22: 14000000 INFO @ Mon, 12 Aug 2019 22:52:25: 14000000 INFO @ Mon, 12 Aug 2019 22:52:29: 13000000 INFO @ Mon, 12 Aug 2019 22:52:30: 15000000 INFO @ Mon, 12 Aug 2019 22:52:32: 15000000 INFO @ Mon, 12 Aug 2019 22:52:38: 16000000 INFO @ Mon, 12 Aug 2019 22:52:38: 14000000 INFO @ Mon, 12 Aug 2019 22:52:40: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 22:52:40: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 22:52:40: #1 total tags in treatment: 16223227 INFO @ Mon, 12 Aug 2019 22:52:40: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:52:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:52:40: #1 tags after filtering in treatment: 16223227 INFO @ Mon, 12 Aug 2019 22:52:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:52:40: #1 finished! INFO @ Mon, 12 Aug 2019 22:52:40: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:52:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:52:40: 16000000 INFO @ Mon, 12 Aug 2019 22:52:41: #2 number of paired peaks: 16 WARNING @ Mon, 12 Aug 2019 22:52:41: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:52:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:52:42: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 22:52:42: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 22:52:42: #1 total tags in treatment: 16223227 INFO @ Mon, 12 Aug 2019 22:52:42: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:52:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:52:42: #1 tags after filtering in treatment: 16223227 INFO @ Mon, 12 Aug 2019 22:52:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:52:42: #1 finished! INFO @ Mon, 12 Aug 2019 22:52:42: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:52:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:52:44: #2 number of paired peaks: 16 WARNING @ Mon, 12 Aug 2019 22:52:44: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:52:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:52:47: 15000000 INFO @ Mon, 12 Aug 2019 22:52:55: 16000000 INFO @ Mon, 12 Aug 2019 22:52:57: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 22:52:57: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 22:52:57: #1 total tags in treatment: 16223227 INFO @ Mon, 12 Aug 2019 22:52:57: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:52:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:52:57: #1 tags after filtering in treatment: 16223227 INFO @ Mon, 12 Aug 2019 22:52:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:52:57: #1 finished! INFO @ Mon, 12 Aug 2019 22:52:57: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:52:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:52:59: #2 number of paired peaks: 16 WARNING @ Mon, 12 Aug 2019 22:52:59: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:52:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495193/SRX495193.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。