Job ID = 6528246 SRX = SRX495187 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:55:14 prefetch.2.10.7: 1) Downloading 'SRR1198692'... 2020-06-29T14:55:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:56:57 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:56:58 prefetch.2.10.7: 'SRR1198692' is valid 2020-06-29T14:56:58 prefetch.2.10.7: 1) 'SRR1198692' was downloaded successfully Read 20417854 spots for SRR1198692/SRR1198692.sra Written 20417854 spots for SRR1198692/SRR1198692.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:06 20417854 reads; of these: 20417854 (100.00%) were unpaired; of these: 4569868 (22.38%) aligned 0 times 13523249 (66.23%) aligned exactly 1 time 2324737 (11.39%) aligned >1 times 77.62% overall alignment rate Time searching: 00:04:06 Overall time: 00:04:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2875584 / 15847986 = 0.1814 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:12:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:12:55: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:12:55: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:13:03: 1000000 INFO @ Tue, 30 Jun 2020 00:13:12: 2000000 INFO @ Tue, 30 Jun 2020 00:13:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:13:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:13:25: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:13:25: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:13:30: 4000000 INFO @ Tue, 30 Jun 2020 00:13:34: 1000000 INFO @ Tue, 30 Jun 2020 00:13:40: 5000000 INFO @ Tue, 30 Jun 2020 00:13:43: 2000000 INFO @ Tue, 30 Jun 2020 00:13:49: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:13:53: 3000000 INFO @ Tue, 30 Jun 2020 00:13:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:13:55: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:13:55: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:13:59: 7000000 INFO @ Tue, 30 Jun 2020 00:14:02: 4000000 INFO @ Tue, 30 Jun 2020 00:14:02: 1000000 INFO @ Tue, 30 Jun 2020 00:14:08: 8000000 INFO @ Tue, 30 Jun 2020 00:14:10: 2000000 INFO @ Tue, 30 Jun 2020 00:14:12: 5000000 INFO @ Tue, 30 Jun 2020 00:14:17: 3000000 INFO @ Tue, 30 Jun 2020 00:14:18: 9000000 INFO @ Tue, 30 Jun 2020 00:14:21: 6000000 INFO @ Tue, 30 Jun 2020 00:14:25: 4000000 INFO @ Tue, 30 Jun 2020 00:14:27: 10000000 INFO @ Tue, 30 Jun 2020 00:14:30: 7000000 INFO @ Tue, 30 Jun 2020 00:14:31: 5000000 INFO @ Tue, 30 Jun 2020 00:14:36: 11000000 INFO @ Tue, 30 Jun 2020 00:14:38: 6000000 INFO @ Tue, 30 Jun 2020 00:14:39: 8000000 INFO @ Tue, 30 Jun 2020 00:14:45: 7000000 INFO @ Tue, 30 Jun 2020 00:14:46: 12000000 INFO @ Tue, 30 Jun 2020 00:14:49: 9000000 INFO @ Tue, 30 Jun 2020 00:14:51: 8000000 INFO @ Tue, 30 Jun 2020 00:14:55: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:14:55: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:14:55: #1 total tags in treatment: 12972402 INFO @ Tue, 30 Jun 2020 00:14:55: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:14:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:14:55: #1 tags after filtering in treatment: 12972402 INFO @ Tue, 30 Jun 2020 00:14:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:14:55: #1 finished! INFO @ Tue, 30 Jun 2020 00:14:55: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:14:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:14:56: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:14:56: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:14:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:14:58: 10000000 INFO @ Tue, 30 Jun 2020 00:14:58: 9000000 INFO @ Tue, 30 Jun 2020 00:15:04: 10000000 INFO @ Tue, 30 Jun 2020 00:15:07: 11000000 INFO @ Tue, 30 Jun 2020 00:15:11: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:15:14: 12000000 INFO @ Tue, 30 Jun 2020 00:15:18: 12000000 INFO @ Tue, 30 Jun 2020 00:15:21: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:15:21: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:15:21: #1 total tags in treatment: 12972402 INFO @ Tue, 30 Jun 2020 00:15:21: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:15:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:15:22: #1 tags after filtering in treatment: 12972402 INFO @ Tue, 30 Jun 2020 00:15:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:15:22: #1 finished! INFO @ Tue, 30 Jun 2020 00:15:22: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:15:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:15:22: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:15:22: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:15:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:15:24: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:15:24: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:15:24: #1 total tags in treatment: 12972402 INFO @ Tue, 30 Jun 2020 00:15:24: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:15:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:15:24: #1 tags after filtering in treatment: 12972402 INFO @ Tue, 30 Jun 2020 00:15:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:15:24: #1 finished! INFO @ Tue, 30 Jun 2020 00:15:24: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:15:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:15:25: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:15:25: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:15:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495187/SRX495187.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。