Job ID = 1304282 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,364,299 reads read : 9,364,299 reads written : 9,364,299 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:43 9364299 reads; of these: 9364299 (100.00%) were unpaired; of these: 148811 (1.59%) aligned 0 times 7102731 (75.85%) aligned exactly 1 time 2112757 (22.56%) aligned >1 times 98.41% overall alignment rate Time searching: 00:02:43 Overall time: 00:02:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1174963 / 9215488 = 0.1275 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:50:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:50:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:50:31: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:50:31: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:50:31: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:50:31: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:50:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:50:31: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:50:31: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:50:41: 1000000 INFO @ Mon, 03 Jun 2019 20:50:41: 1000000 INFO @ Mon, 03 Jun 2019 20:50:41: 1000000 INFO @ Mon, 03 Jun 2019 20:50:50: 2000000 INFO @ Mon, 03 Jun 2019 20:50:50: 2000000 INFO @ Mon, 03 Jun 2019 20:50:51: 2000000 INFO @ Mon, 03 Jun 2019 20:51:00: 3000000 INFO @ Mon, 03 Jun 2019 20:51:00: 3000000 INFO @ Mon, 03 Jun 2019 20:51:01: 3000000 INFO @ Mon, 03 Jun 2019 20:51:09: 4000000 INFO @ Mon, 03 Jun 2019 20:51:09: 4000000 INFO @ Mon, 03 Jun 2019 20:51:11: 4000000 INFO @ Mon, 03 Jun 2019 20:51:19: 5000000 INFO @ Mon, 03 Jun 2019 20:51:19: 5000000 INFO @ Mon, 03 Jun 2019 20:51:21: 5000000 INFO @ Mon, 03 Jun 2019 20:51:28: 6000000 INFO @ Mon, 03 Jun 2019 20:51:28: 6000000 INFO @ Mon, 03 Jun 2019 20:51:31: 6000000 INFO @ Mon, 03 Jun 2019 20:51:38: 7000000 INFO @ Mon, 03 Jun 2019 20:51:38: 7000000 INFO @ Mon, 03 Jun 2019 20:51:41: 7000000 INFO @ Mon, 03 Jun 2019 20:51:47: 8000000 INFO @ Mon, 03 Jun 2019 20:51:47: 8000000 INFO @ Mon, 03 Jun 2019 20:51:47: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:51:47: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:51:47: #1 total tags in treatment: 8040525 INFO @ Mon, 03 Jun 2019 20:51:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:51:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:51:47: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:51:47: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:51:47: #1 total tags in treatment: 8040525 INFO @ Mon, 03 Jun 2019 20:51:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:51:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:51:47: #1 tags after filtering in treatment: 8040525 INFO @ Mon, 03 Jun 2019 20:51:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:51:47: #1 finished! INFO @ Mon, 03 Jun 2019 20:51:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:51:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:51:47: #1 tags after filtering in treatment: 8040525 INFO @ Mon, 03 Jun 2019 20:51:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:51:47: #1 finished! INFO @ Mon, 03 Jun 2019 20:51:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:51:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:51:48: #2 number of paired peaks: 63 WARNING @ Mon, 03 Jun 2019 20:51:48: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:51:48: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 20:51:48: #2 number of paired peaks: 63 WARNING @ Mon, 03 Jun 2019 20:51:48: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:51:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:51:50: 8000000 INFO @ Mon, 03 Jun 2019 20:51:51: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 20:51:51: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 20:51:51: #1 total tags in treatment: 8040525 INFO @ Mon, 03 Jun 2019 20:51:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:51:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:51:51: #1 tags after filtering in treatment: 8040525 INFO @ Mon, 03 Jun 2019 20:51:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:51:51: #1 finished! INFO @ Mon, 03 Jun 2019 20:51:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:51:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:51:52: #2 number of paired peaks: 63 WARNING @ Mon, 03 Jun 2019 20:51:52: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:51:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495182/SRX495182.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。