Job ID = 6528206 SRX = SRX4933895 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:47:25 prefetch.2.10.7: 1) Downloading 'SRR8107286'... 2020-06-29T14:47:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:50:06 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:50:06 prefetch.2.10.7: 1) 'SRR8107286' was downloaded successfully Read 30600201 spots for SRR8107286/SRR8107286.sra Written 30600201 spots for SRR8107286/SRR8107286.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:18 30600201 reads; of these: 30600201 (100.00%) were unpaired; of these: 1248243 (4.08%) aligned 0 times 21714920 (70.96%) aligned exactly 1 time 7637038 (24.96%) aligned >1 times 95.92% overall alignment rate Time searching: 00:10:18 Overall time: 00:10:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6265638 / 29351958 = 0.2135 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:17:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:17:41: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:17:41: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:17:46: 1000000 INFO @ Tue, 30 Jun 2020 00:17:51: 2000000 INFO @ Tue, 30 Jun 2020 00:17:56: 3000000 INFO @ Tue, 30 Jun 2020 00:18:01: 4000000 INFO @ Tue, 30 Jun 2020 00:18:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:11: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:11: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:11: 6000000 INFO @ Tue, 30 Jun 2020 00:18:16: 7000000 INFO @ Tue, 30 Jun 2020 00:18:16: 1000000 INFO @ Tue, 30 Jun 2020 00:18:21: 8000000 INFO @ Tue, 30 Jun 2020 00:18:22: 2000000 INFO @ Tue, 30 Jun 2020 00:18:27: 9000000 INFO @ Tue, 30 Jun 2020 00:18:27: 3000000 INFO @ Tue, 30 Jun 2020 00:18:32: 10000000 INFO @ Tue, 30 Jun 2020 00:18:32: 4000000 INFO @ Tue, 30 Jun 2020 00:18:37: 11000000 INFO @ Tue, 30 Jun 2020 00:18:37: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:41: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:41: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:43: 12000000 INFO @ Tue, 30 Jun 2020 00:18:43: 6000000 INFO @ Tue, 30 Jun 2020 00:18:47: 1000000 INFO @ Tue, 30 Jun 2020 00:18:48: 13000000 INFO @ Tue, 30 Jun 2020 00:18:48: 7000000 INFO @ Tue, 30 Jun 2020 00:18:53: 2000000 INFO @ Tue, 30 Jun 2020 00:18:54: 14000000 INFO @ Tue, 30 Jun 2020 00:18:54: 8000000 INFO @ Tue, 30 Jun 2020 00:18:59: 15000000 INFO @ Tue, 30 Jun 2020 00:19:00: 9000000 INFO @ Tue, 30 Jun 2020 00:19:00: 3000000 INFO @ Tue, 30 Jun 2020 00:19:05: 16000000 INFO @ Tue, 30 Jun 2020 00:19:05: 10000000 INFO @ Tue, 30 Jun 2020 00:19:06: 4000000 INFO @ Tue, 30 Jun 2020 00:19:10: 17000000 INFO @ Tue, 30 Jun 2020 00:19:11: 11000000 INFO @ Tue, 30 Jun 2020 00:19:12: 5000000 INFO @ Tue, 30 Jun 2020 00:19:16: 18000000 INFO @ Tue, 30 Jun 2020 00:19:16: 12000000 INFO @ Tue, 30 Jun 2020 00:19:18: 6000000 INFO @ Tue, 30 Jun 2020 00:19:21: 19000000 INFO @ Tue, 30 Jun 2020 00:19:22: 13000000 INFO @ Tue, 30 Jun 2020 00:19:25: 7000000 INFO @ Tue, 30 Jun 2020 00:19:27: 20000000 INFO @ Tue, 30 Jun 2020 00:19:27: 14000000 INFO @ Tue, 30 Jun 2020 00:19:31: 8000000 INFO @ Tue, 30 Jun 2020 00:19:32: 21000000 INFO @ Tue, 30 Jun 2020 00:19:33: 15000000 INFO @ Tue, 30 Jun 2020 00:19:37: 9000000 INFO @ Tue, 30 Jun 2020 00:19:38: 22000000 INFO @ Tue, 30 Jun 2020 00:19:38: 16000000 INFO @ Tue, 30 Jun 2020 00:19:43: 23000000 INFO @ Tue, 30 Jun 2020 00:19:44: 10000000 INFO @ Tue, 30 Jun 2020 00:19:44: 17000000 INFO @ Tue, 30 Jun 2020 00:19:44: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:19:44: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:19:44: #1 total tags in treatment: 23086320 INFO @ Tue, 30 Jun 2020 00:19:44: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:19:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:19:44: #1 tags after filtering in treatment: 23086320 INFO @ Tue, 30 Jun 2020 00:19:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:19:44: #1 finished! INFO @ Tue, 30 Jun 2020 00:19:44: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:19:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:19:46: #2 number of paired peaks: 15 WARNING @ Tue, 30 Jun 2020 00:19:46: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:19:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:19:50: 18000000 INFO @ Tue, 30 Jun 2020 00:19:50: 11000000 INFO @ Tue, 30 Jun 2020 00:19:55: 19000000 INFO @ Tue, 30 Jun 2020 00:19:56: 12000000 INFO @ Tue, 30 Jun 2020 00:20:01: 20000000 INFO @ Tue, 30 Jun 2020 00:20:03: 13000000 INFO @ Tue, 30 Jun 2020 00:20:06: 21000000 INFO @ Tue, 30 Jun 2020 00:20:09: 14000000 INFO @ Tue, 30 Jun 2020 00:20:12: 22000000 INFO @ Tue, 30 Jun 2020 00:20:15: 15000000 INFO @ Tue, 30 Jun 2020 00:20:17: 23000000 INFO @ Tue, 30 Jun 2020 00:20:18: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:20:18: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:20:18: #1 total tags in treatment: 23086320 INFO @ Tue, 30 Jun 2020 00:20:18: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:20:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:20:18: #1 tags after filtering in treatment: 23086320 INFO @ Tue, 30 Jun 2020 00:20:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:20:18: #1 finished! INFO @ Tue, 30 Jun 2020 00:20:18: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:20:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:20:20: #2 number of paired peaks: 15 WARNING @ Tue, 30 Jun 2020 00:20:20: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:20:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:20:22: 16000000 INFO @ Tue, 30 Jun 2020 00:20:28: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:20:34: 18000000 INFO @ Tue, 30 Jun 2020 00:20:40: 19000000 INFO @ Tue, 30 Jun 2020 00:20:46: 20000000 INFO @ Tue, 30 Jun 2020 00:20:53: 21000000 INFO @ Tue, 30 Jun 2020 00:20:59: 22000000 INFO @ Tue, 30 Jun 2020 00:21:05: 23000000 INFO @ Tue, 30 Jun 2020 00:21:06: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:21:06: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:21:06: #1 total tags in treatment: 23086320 INFO @ Tue, 30 Jun 2020 00:21:06: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:21:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:21:06: #1 tags after filtering in treatment: 23086320 INFO @ Tue, 30 Jun 2020 00:21:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:21:06: #1 finished! INFO @ Tue, 30 Jun 2020 00:21:06: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:21:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:21:07: #2 number of paired peaks: 15 WARNING @ Tue, 30 Jun 2020 00:21:07: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:21:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933895/SRX4933895.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。