Job ID = 6528205 SRX = SRX4933894 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:43:28 prefetch.2.10.7: 1) Downloading 'SRR8107285'... 2020-06-29T14:43:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:46:40 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:46:40 prefetch.2.10.7: 1) 'SRR8107285' was downloaded successfully Read 24448370 spots for SRR8107285/SRR8107285.sra Written 24448370 spots for SRR8107285/SRR8107285.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:43 24448370 reads; of these: 24448370 (100.00%) were unpaired; of these: 1081000 (4.42%) aligned 0 times 17641146 (72.16%) aligned exactly 1 time 5726224 (23.42%) aligned >1 times 95.58% overall alignment rate Time searching: 00:07:43 Overall time: 00:07:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5060236 / 23367370 = 0.2166 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:33: 1000000 INFO @ Tue, 30 Jun 2020 00:07:40: 2000000 INFO @ Tue, 30 Jun 2020 00:07:46: 3000000 INFO @ Tue, 30 Jun 2020 00:07:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:56: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:56: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:59: 5000000 INFO @ Tue, 30 Jun 2020 00:08:04: 1000000 INFO @ Tue, 30 Jun 2020 00:08:06: 6000000 INFO @ Tue, 30 Jun 2020 00:08:11: 2000000 INFO @ Tue, 30 Jun 2020 00:08:13: 7000000 INFO @ Tue, 30 Jun 2020 00:08:18: 3000000 INFO @ Tue, 30 Jun 2020 00:08:20: 8000000 INFO @ Tue, 30 Jun 2020 00:08:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:08:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:08:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:08:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:08:27: 9000000 INFO @ Tue, 30 Jun 2020 00:08:32: 5000000 INFO @ Tue, 30 Jun 2020 00:08:33: 1000000 INFO @ Tue, 30 Jun 2020 00:08:33: 10000000 INFO @ Tue, 30 Jun 2020 00:08:39: 6000000 INFO @ Tue, 30 Jun 2020 00:08:40: 2000000 INFO @ Tue, 30 Jun 2020 00:08:40: 11000000 INFO @ Tue, 30 Jun 2020 00:08:46: 7000000 INFO @ Tue, 30 Jun 2020 00:08:47: 3000000 INFO @ Tue, 30 Jun 2020 00:08:47: 12000000 INFO @ Tue, 30 Jun 2020 00:08:53: 8000000 INFO @ Tue, 30 Jun 2020 00:08:54: 4000000 INFO @ Tue, 30 Jun 2020 00:08:54: 13000000 INFO @ Tue, 30 Jun 2020 00:09:00: 9000000 INFO @ Tue, 30 Jun 2020 00:09:01: 5000000 INFO @ Tue, 30 Jun 2020 00:09:01: 14000000 INFO @ Tue, 30 Jun 2020 00:09:06: 10000000 INFO @ Tue, 30 Jun 2020 00:09:08: 6000000 INFO @ Tue, 30 Jun 2020 00:09:08: 15000000 INFO @ Tue, 30 Jun 2020 00:09:14: 11000000 INFO @ Tue, 30 Jun 2020 00:09:15: 16000000 INFO @ Tue, 30 Jun 2020 00:09:15: 7000000 INFO @ Tue, 30 Jun 2020 00:09:21: 12000000 INFO @ Tue, 30 Jun 2020 00:09:22: 8000000 INFO @ Tue, 30 Jun 2020 00:09:22: 17000000 INFO @ Tue, 30 Jun 2020 00:09:27: 13000000 INFO @ Tue, 30 Jun 2020 00:09:29: 9000000 INFO @ Tue, 30 Jun 2020 00:09:29: 18000000 INFO @ Tue, 30 Jun 2020 00:09:31: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:09:31: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:09:31: #1 total tags in treatment: 18307134 INFO @ Tue, 30 Jun 2020 00:09:31: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:31: #1 tags after filtering in treatment: 18307134 INFO @ Tue, 30 Jun 2020 00:09:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:31: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:32: #2 number of paired peaks: 22 WARNING @ Tue, 30 Jun 2020 00:09:32: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:09:35: 14000000 INFO @ Tue, 30 Jun 2020 00:09:35: 10000000 INFO @ Tue, 30 Jun 2020 00:09:41: 15000000 INFO @ Tue, 30 Jun 2020 00:09:42: 11000000 INFO @ Tue, 30 Jun 2020 00:09:48: 16000000 INFO @ Tue, 30 Jun 2020 00:09:49: 12000000 INFO @ Tue, 30 Jun 2020 00:09:55: 17000000 INFO @ Tue, 30 Jun 2020 00:09:56: 13000000 INFO @ Tue, 30 Jun 2020 00:10:02: 18000000 INFO @ Tue, 30 Jun 2020 00:10:03: 14000000 INFO @ Tue, 30 Jun 2020 00:10:04: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:10:04: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:10:04: #1 total tags in treatment: 18307134 INFO @ Tue, 30 Jun 2020 00:10:04: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:10:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:10:05: #1 tags after filtering in treatment: 18307134 INFO @ Tue, 30 Jun 2020 00:10:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:10:05: #1 finished! INFO @ Tue, 30 Jun 2020 00:10:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:10:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:10:06: #2 number of paired peaks: 22 WARNING @ Tue, 30 Jun 2020 00:10:06: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:10:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.10_peaks.narrowPeak: No such file or directory BedGraph に変換しました。 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) BigWig に変換中... rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:10:09: 15000000 INFO @ Tue, 30 Jun 2020 00:10:16: 16000000 INFO @ Tue, 30 Jun 2020 00:10:22: 17000000 INFO @ Tue, 30 Jun 2020 00:10:29: 18000000 INFO @ Tue, 30 Jun 2020 00:10:31: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:10:31: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:10:31: #1 total tags in treatment: 18307134 INFO @ Tue, 30 Jun 2020 00:10:31: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:10:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:10:31: #1 tags after filtering in treatment: 18307134 INFO @ Tue, 30 Jun 2020 00:10:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:10:31: #1 finished! INFO @ Tue, 30 Jun 2020 00:10:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:10:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:10:32: #2 number of paired peaks: 22 WARNING @ Tue, 30 Jun 2020 00:10:32: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:10:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933894/SRX4933894.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。