Job ID = 6528204 SRX = SRX4933893 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:42:24 prefetch.2.10.7: 1) Downloading 'SRR8107284'... 2020-06-29T14:42:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:46:33 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:46:33 prefetch.2.10.7: 1) 'SRR8107284' was downloaded successfully Read 33043080 spots for SRR8107284/SRR8107284.sra Written 33043080 spots for SRR8107284/SRR8107284.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:12:29 33043080 reads; of these: 33043080 (100.00%) were unpaired; of these: 791898 (2.40%) aligned 0 times 23513838 (71.16%) aligned exactly 1 time 8737344 (26.44%) aligned >1 times 97.60% overall alignment rate Time searching: 00:12:30 Overall time: 00:12:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8511582 / 32251182 = 0.2639 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:21:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:21:48: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:21:48: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:21:57: 1000000 INFO @ Tue, 30 Jun 2020 00:22:06: 2000000 INFO @ Tue, 30 Jun 2020 00:22:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:22:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:22:18: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:22:18: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:22:25: 4000000 INFO @ Tue, 30 Jun 2020 00:22:27: 1000000 INFO @ Tue, 30 Jun 2020 00:22:34: 5000000 INFO @ Tue, 30 Jun 2020 00:22:37: 2000000 INFO @ Tue, 30 Jun 2020 00:22:44: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:22:47: 3000000 INFO @ Tue, 30 Jun 2020 00:22:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:22:48: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:22:48: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:22:53: 7000000 INFO @ Tue, 30 Jun 2020 00:22:54: 1000000 INFO @ Tue, 30 Jun 2020 00:22:56: 4000000 INFO @ Tue, 30 Jun 2020 00:23:01: 2000000 INFO @ Tue, 30 Jun 2020 00:23:02: 8000000 INFO @ Tue, 30 Jun 2020 00:23:05: 5000000 INFO @ Tue, 30 Jun 2020 00:23:07: 3000000 INFO @ Tue, 30 Jun 2020 00:23:12: 9000000 INFO @ Tue, 30 Jun 2020 00:23:14: 4000000 INFO @ Tue, 30 Jun 2020 00:23:14: 6000000 INFO @ Tue, 30 Jun 2020 00:23:20: 5000000 INFO @ Tue, 30 Jun 2020 00:23:21: 10000000 INFO @ Tue, 30 Jun 2020 00:23:24: 7000000 INFO @ Tue, 30 Jun 2020 00:23:27: 6000000 INFO @ Tue, 30 Jun 2020 00:23:30: 11000000 INFO @ Tue, 30 Jun 2020 00:23:33: 8000000 INFO @ Tue, 30 Jun 2020 00:23:33: 7000000 INFO @ Tue, 30 Jun 2020 00:23:39: 12000000 INFO @ Tue, 30 Jun 2020 00:23:40: 8000000 INFO @ Tue, 30 Jun 2020 00:23:42: 9000000 INFO @ Tue, 30 Jun 2020 00:23:46: 9000000 INFO @ Tue, 30 Jun 2020 00:23:48: 13000000 INFO @ Tue, 30 Jun 2020 00:23:51: 10000000 INFO @ Tue, 30 Jun 2020 00:23:53: 10000000 INFO @ Tue, 30 Jun 2020 00:23:57: 14000000 INFO @ Tue, 30 Jun 2020 00:23:59: 11000000 INFO @ Tue, 30 Jun 2020 00:24:00: 11000000 INFO @ Tue, 30 Jun 2020 00:24:05: 12000000 INFO @ Tue, 30 Jun 2020 00:24:07: 15000000 INFO @ Tue, 30 Jun 2020 00:24:09: 12000000 INFO @ Tue, 30 Jun 2020 00:24:12: 13000000 INFO @ Tue, 30 Jun 2020 00:24:16: 16000000 INFO @ Tue, 30 Jun 2020 00:24:18: 14000000 INFO @ Tue, 30 Jun 2020 00:24:18: 13000000 INFO @ Tue, 30 Jun 2020 00:24:25: 15000000 INFO @ Tue, 30 Jun 2020 00:24:25: 17000000 INFO @ Tue, 30 Jun 2020 00:24:27: 14000000 INFO @ Tue, 30 Jun 2020 00:24:31: 16000000 INFO @ Tue, 30 Jun 2020 00:24:34: 18000000 INFO @ Tue, 30 Jun 2020 00:24:37: 15000000 INFO @ Tue, 30 Jun 2020 00:24:37: 17000000 INFO @ Tue, 30 Jun 2020 00:24:43: 19000000 INFO @ Tue, 30 Jun 2020 00:24:44: 18000000 INFO @ Tue, 30 Jun 2020 00:24:46: 16000000 INFO @ Tue, 30 Jun 2020 00:24:50: 19000000 INFO @ Tue, 30 Jun 2020 00:24:52: 20000000 INFO @ Tue, 30 Jun 2020 00:24:55: 17000000 INFO @ Tue, 30 Jun 2020 00:24:57: 20000000 INFO @ Tue, 30 Jun 2020 00:25:02: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:25:03: 21000000 INFO @ Tue, 30 Jun 2020 00:25:04: 18000000 INFO @ Tue, 30 Jun 2020 00:25:09: 22000000 INFO @ Tue, 30 Jun 2020 00:25:11: 22000000 INFO @ Tue, 30 Jun 2020 00:25:13: 19000000 INFO @ Tue, 30 Jun 2020 00:25:16: 23000000 INFO @ Tue, 30 Jun 2020 00:25:20: 23000000 INFO @ Tue, 30 Jun 2020 00:25:21: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:25:21: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:25:21: #1 total tags in treatment: 23739600 INFO @ Tue, 30 Jun 2020 00:25:21: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:25:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:25:21: #1 tags after filtering in treatment: 23739600 INFO @ Tue, 30 Jun 2020 00:25:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:25:21: #1 finished! INFO @ Tue, 30 Jun 2020 00:25:21: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:25:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:25:22: 20000000 INFO @ Tue, 30 Jun 2020 00:25:23: #2 number of paired peaks: 12 WARNING @ Tue, 30 Jun 2020 00:25:23: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:25:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:25:26: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:25:26: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:25:26: #1 total tags in treatment: 23739600 INFO @ Tue, 30 Jun 2020 00:25:26: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:25:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:25:27: #1 tags after filtering in treatment: 23739600 INFO @ Tue, 30 Jun 2020 00:25:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:25:27: #1 finished! INFO @ Tue, 30 Jun 2020 00:25:27: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:25:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:25:28: #2 number of paired peaks: 12 WARNING @ Tue, 30 Jun 2020 00:25:28: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:25:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:25:31: 21000000 INFO @ Tue, 30 Jun 2020 00:25:39: 22000000 INFO @ Tue, 30 Jun 2020 00:25:48: 23000000 INFO @ Tue, 30 Jun 2020 00:25:54: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:25:54: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:25:54: #1 total tags in treatment: 23739600 INFO @ Tue, 30 Jun 2020 00:25:54: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:25:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:25:54: #1 tags after filtering in treatment: 23739600 INFO @ Tue, 30 Jun 2020 00:25:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:25:54: #1 finished! INFO @ Tue, 30 Jun 2020 00:25:54: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:25:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:25:56: #2 number of paired peaks: 12 WARNING @ Tue, 30 Jun 2020 00:25:56: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:25:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933893/SRX4933893.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。