Job ID = 6528203 SRX = SRX4933888 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:27:06 prefetch.2.10.7: 1) Downloading 'SRR8107278'... 2020-06-29T14:27:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:30:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:30:37 prefetch.2.10.7: 1) 'SRR8107278' was downloaded successfully Read 16406061 spots for SRR8107278/SRR8107278.sra Written 16406061 spots for SRR8107278/SRR8107278.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:44 16406061 reads; of these: 16406061 (100.00%) were unpaired; of these: 727000 (4.43%) aligned 0 times 11727663 (71.48%) aligned exactly 1 time 3951398 (24.08%) aligned >1 times 95.57% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3418117 / 15679061 = 0.2180 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:12: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:12: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:19: 1000000 INFO @ Mon, 29 Jun 2020 23:47:26: 2000000 INFO @ Mon, 29 Jun 2020 23:47:33: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:40: 4000000 INFO @ Mon, 29 Jun 2020 23:47:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:42: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:42: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:48: 5000000 INFO @ Mon, 29 Jun 2020 23:47:49: 1000000 INFO @ Mon, 29 Jun 2020 23:47:55: 6000000 INFO @ Mon, 29 Jun 2020 23:47:56: 2000000 INFO @ Mon, 29 Jun 2020 23:48:02: 7000000 INFO @ Mon, 29 Jun 2020 23:48:03: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:09: 8000000 INFO @ Mon, 29 Jun 2020 23:48:11: 4000000 INFO @ Mon, 29 Jun 2020 23:48:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:12: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:12: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:17: 9000000 INFO @ Mon, 29 Jun 2020 23:48:18: 5000000 INFO @ Mon, 29 Jun 2020 23:48:19: 1000000 INFO @ Mon, 29 Jun 2020 23:48:24: 10000000 INFO @ Mon, 29 Jun 2020 23:48:26: 6000000 INFO @ Mon, 29 Jun 2020 23:48:27: 2000000 INFO @ Mon, 29 Jun 2020 23:48:32: 11000000 INFO @ Mon, 29 Jun 2020 23:48:33: 7000000 INFO @ Mon, 29 Jun 2020 23:48:34: 3000000 INFO @ Mon, 29 Jun 2020 23:48:39: 12000000 INFO @ Mon, 29 Jun 2020 23:48:40: 8000000 INFO @ Mon, 29 Jun 2020 23:48:41: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:48:41: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:48:41: #1 total tags in treatment: 12260944 INFO @ Mon, 29 Jun 2020 23:48:41: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:48:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:48:41: #1 tags after filtering in treatment: 12260944 INFO @ Mon, 29 Jun 2020 23:48:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:48:41: #1 finished! INFO @ Mon, 29 Jun 2020 23:48:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:48:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:48:42: 4000000 INFO @ Mon, 29 Jun 2020 23:48:42: #2 number of paired peaks: 16 WARNING @ Mon, 29 Jun 2020 23:48:42: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:48:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:48:48: 9000000 INFO @ Mon, 29 Jun 2020 23:48:49: 5000000 INFO @ Mon, 29 Jun 2020 23:48:55: 10000000 INFO @ Mon, 29 Jun 2020 23:48:56: 6000000 INFO @ Mon, 29 Jun 2020 23:49:02: 11000000 INFO @ Mon, 29 Jun 2020 23:49:03: 7000000 INFO @ Mon, 29 Jun 2020 23:49:09: 12000000 INFO @ Mon, 29 Jun 2020 23:49:10: 8000000 INFO @ Mon, 29 Jun 2020 23:49:11: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:49:11: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:49:11: #1 total tags in treatment: 12260944 INFO @ Mon, 29 Jun 2020 23:49:11: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:11: #1 tags after filtering in treatment: 12260944 INFO @ Mon, 29 Jun 2020 23:49:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:11: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:11: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:12: #2 number of paired peaks: 16 WARNING @ Mon, 29 Jun 2020 23:49:12: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:49:17: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:49:24: 10000000 INFO @ Mon, 29 Jun 2020 23:49:31: 11000000 INFO @ Mon, 29 Jun 2020 23:49:37: 12000000 INFO @ Mon, 29 Jun 2020 23:49:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:49:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:49:39: #1 total tags in treatment: 12260944 INFO @ Mon, 29 Jun 2020 23:49:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:39: #1 tags after filtering in treatment: 12260944 INFO @ Mon, 29 Jun 2020 23:49:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:40: #2 number of paired peaks: 16 WARNING @ Mon, 29 Jun 2020 23:49:40: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933888/SRX4933888.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。