Job ID = 6528167 SRX = SRX4933883 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:34:06 prefetch.2.10.7: 1) Downloading 'SRR8107273'... 2020-06-29T14:34:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:36:23 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:36:23 prefetch.2.10.7: 1) 'SRR8107273' was downloaded successfully Read 14492663 spots for SRR8107273/SRR8107273.sra Written 14492663 spots for SRR8107273/SRR8107273.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:14 14492663 reads; of these: 14492663 (100.00%) were unpaired; of these: 2369642 (16.35%) aligned 0 times 8073128 (55.70%) aligned exactly 1 time 4049893 (27.94%) aligned >1 times 83.65% overall alignment rate Time searching: 00:05:14 Overall time: 00:05:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2995054 / 12123021 = 0.2471 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:54: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:54: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:59: 1000000 INFO @ Mon, 29 Jun 2020 23:49:04: 2000000 INFO @ Mon, 29 Jun 2020 23:49:10: 3000000 INFO @ Mon, 29 Jun 2020 23:49:15: 4000000 INFO @ Mon, 29 Jun 2020 23:49:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:49:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:49:23: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:49:23: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:49:26: 6000000 INFO @ Mon, 29 Jun 2020 23:49:29: 1000000 INFO @ Mon, 29 Jun 2020 23:49:32: 7000000 INFO @ Mon, 29 Jun 2020 23:49:35: 2000000 INFO @ Mon, 29 Jun 2020 23:49:38: 8000000 INFO @ Mon, 29 Jun 2020 23:49:41: 3000000 INFO @ Mon, 29 Jun 2020 23:49:44: 9000000 INFO @ Mon, 29 Jun 2020 23:49:45: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:49:45: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:49:45: #1 total tags in treatment: 9127967 INFO @ Mon, 29 Jun 2020 23:49:45: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:45: #1 tags after filtering in treatment: 9127967 INFO @ Mon, 29 Jun 2020 23:49:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:45: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:46: #2 number of paired peaks: 21 WARNING @ Mon, 29 Jun 2020 23:49:46: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:49:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:49:53: 5000000 INFO @ Mon, 29 Jun 2020 23:49:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:49:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:49:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:49:59: 1000000 INFO @ Mon, 29 Jun 2020 23:49:59: 6000000 INFO @ Mon, 29 Jun 2020 23:50:05: 2000000 INFO @ Mon, 29 Jun 2020 23:50:05: 7000000 INFO @ Mon, 29 Jun 2020 23:50:11: 3000000 INFO @ Mon, 29 Jun 2020 23:50:11: 8000000 INFO @ Mon, 29 Jun 2020 23:50:17: 4000000 INFO @ Mon, 29 Jun 2020 23:50:17: 9000000 INFO @ Mon, 29 Jun 2020 23:50:18: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:50:18: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:50:18: #1 total tags in treatment: 9127967 INFO @ Mon, 29 Jun 2020 23:50:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:18: #1 tags after filtering in treatment: 9127967 INFO @ Mon, 29 Jun 2020 23:50:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:18: #2 number of paired peaks: 21 WARNING @ Mon, 29 Jun 2020 23:50:18: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:50:22: 5000000 INFO @ Mon, 29 Jun 2020 23:50:28: 6000000 INFO @ Mon, 29 Jun 2020 23:50:34: 7000000 INFO @ Mon, 29 Jun 2020 23:50:39: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:50:45: 9000000 INFO @ Mon, 29 Jun 2020 23:50:46: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:50:46: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:50:46: #1 total tags in treatment: 9127967 INFO @ Mon, 29 Jun 2020 23:50:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:46: #1 tags after filtering in treatment: 9127967 INFO @ Mon, 29 Jun 2020 23:50:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:46: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:46: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:46: #2 number of paired peaks: 21 WARNING @ Mon, 29 Jun 2020 23:50:46: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933883/SRX4933883.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。