Job ID = 6528163 SRX = SRX4933871 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:46:13 prefetch.2.10.7: 1) Downloading 'SRR8107260'... 2020-06-29T14:46:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:47:17 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:47:17 prefetch.2.10.7: 'SRR8107260' is valid 2020-06-29T14:47:17 prefetch.2.10.7: 1) 'SRR8107260' was downloaded successfully Read 9937103 spots for SRR8107260/SRR8107260.sra Written 9937103 spots for SRR8107260/SRR8107260.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:42 9937103 reads; of these: 9937103 (100.00%) were unpaired; of these: 4656935 (46.86%) aligned 0 times 3418923 (34.41%) aligned exactly 1 time 1861245 (18.73%) aligned >1 times 53.14% overall alignment rate Time searching: 00:02:42 Overall time: 00:02:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1824159 / 5280168 = 0.3455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:54:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:54:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:54:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:54:37: 1000000 INFO @ Mon, 29 Jun 2020 23:54:43: 2000000 INFO @ Mon, 29 Jun 2020 23:54:48: 3000000 INFO @ Mon, 29 Jun 2020 23:54:50: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:54:50: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:54:50: #1 total tags in treatment: 3456009 INFO @ Mon, 29 Jun 2020 23:54:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:50: #1 tags after filtering in treatment: 3456009 INFO @ Mon, 29 Jun 2020 23:54:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:51: #2 number of paired peaks: 59 WARNING @ Mon, 29 Jun 2020 23:54:51: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:08: 1000000 INFO @ Mon, 29 Jun 2020 23:55:13: 2000000 INFO @ Mon, 29 Jun 2020 23:55:18: 3000000 INFO @ Mon, 29 Jun 2020 23:55:21: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:55:21: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:55:21: #1 total tags in treatment: 3456009 INFO @ Mon, 29 Jun 2020 23:55:21: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:55:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:55:21: #1 tags after filtering in treatment: 3456009 INFO @ Mon, 29 Jun 2020 23:55:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:55:21: #1 finished! INFO @ Mon, 29 Jun 2020 23:55:21: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:55:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:55:21: #2 number of paired peaks: 59 WARNING @ Mon, 29 Jun 2020 23:55:21: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:55:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:37: 1000000 INFO @ Mon, 29 Jun 2020 23:55:42: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:55:48: 3000000 INFO @ Mon, 29 Jun 2020 23:55:50: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:55:50: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:55:50: #1 total tags in treatment: 3456009 INFO @ Mon, 29 Jun 2020 23:55:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:55:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:55:50: #1 tags after filtering in treatment: 3456009 INFO @ Mon, 29 Jun 2020 23:55:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:55:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:55:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:55:50: #2 number of paired peaks: 59 WARNING @ Mon, 29 Jun 2020 23:55:50: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:55:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4933871/SRX4933871.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。