Job ID = 1301738 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 29,522,238 reads read : 29,522,238 reads written : 29,522,238 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:26 29522238 reads; of these: 29522238 (100.00%) were unpaired; of these: 1277770 (4.33%) aligned 0 times 26426240 (89.51%) aligned exactly 1 time 1818228 (6.16%) aligned >1 times 95.67% overall alignment rate Time searching: 00:05:26 Overall time: 00:05:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3294705 / 28244468 = 0.1166 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:17:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:17:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:17:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:17:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:17:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:17:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:17:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:17:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:17:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:18:05: 1000000 INFO @ Mon, 03 Jun 2019 20:18:06: 1000000 INFO @ Mon, 03 Jun 2019 20:18:06: 1000000 INFO @ Mon, 03 Jun 2019 20:18:12: 2000000 INFO @ Mon, 03 Jun 2019 20:18:15: 2000000 INFO @ Mon, 03 Jun 2019 20:18:15: 2000000 INFO @ Mon, 03 Jun 2019 20:18:18: 3000000 INFO @ Mon, 03 Jun 2019 20:18:24: 3000000 INFO @ Mon, 03 Jun 2019 20:18:24: 3000000 INFO @ Mon, 03 Jun 2019 20:18:24: 4000000 INFO @ Mon, 03 Jun 2019 20:18:31: 5000000 INFO @ Mon, 03 Jun 2019 20:18:32: 4000000 INFO @ Mon, 03 Jun 2019 20:18:33: 4000000 INFO @ Mon, 03 Jun 2019 20:18:39: 6000000 INFO @ Mon, 03 Jun 2019 20:18:41: 5000000 INFO @ Mon, 03 Jun 2019 20:18:42: 5000000 INFO @ Mon, 03 Jun 2019 20:18:46: 7000000 INFO @ Mon, 03 Jun 2019 20:18:50: 6000000 INFO @ Mon, 03 Jun 2019 20:18:50: 6000000 INFO @ Mon, 03 Jun 2019 20:18:53: 8000000 INFO @ Mon, 03 Jun 2019 20:18:59: 7000000 INFO @ Mon, 03 Jun 2019 20:18:59: 7000000 INFO @ Mon, 03 Jun 2019 20:18:59: 9000000 INFO @ Mon, 03 Jun 2019 20:19:06: 10000000 INFO @ Mon, 03 Jun 2019 20:19:07: 8000000 INFO @ Mon, 03 Jun 2019 20:19:08: 8000000 INFO @ Mon, 03 Jun 2019 20:19:12: 11000000 INFO @ Mon, 03 Jun 2019 20:19:14: 9000000 INFO @ Mon, 03 Jun 2019 20:19:16: 9000000 INFO @ Mon, 03 Jun 2019 20:19:19: 12000000 INFO @ Mon, 03 Jun 2019 20:19:22: 10000000 INFO @ Mon, 03 Jun 2019 20:19:24: 10000000 INFO @ Mon, 03 Jun 2019 20:19:25: 13000000 INFO @ Mon, 03 Jun 2019 20:19:30: 11000000 INFO @ Mon, 03 Jun 2019 20:19:32: 14000000 INFO @ Mon, 03 Jun 2019 20:19:32: 11000000 INFO @ Mon, 03 Jun 2019 20:19:37: 12000000 INFO @ Mon, 03 Jun 2019 20:19:38: 15000000 INFO @ Mon, 03 Jun 2019 20:19:40: 12000000 INFO @ Mon, 03 Jun 2019 20:19:44: 16000000 INFO @ Mon, 03 Jun 2019 20:19:45: 13000000 INFO @ Mon, 03 Jun 2019 20:19:48: 13000000 INFO @ Mon, 03 Jun 2019 20:19:51: 17000000 INFO @ Mon, 03 Jun 2019 20:19:52: 14000000 INFO @ Mon, 03 Jun 2019 20:19:56: 14000000 INFO @ Mon, 03 Jun 2019 20:19:58: 18000000 INFO @ Mon, 03 Jun 2019 20:20:00: 15000000 INFO @ Mon, 03 Jun 2019 20:20:04: 15000000 INFO @ Mon, 03 Jun 2019 20:20:05: 19000000 INFO @ Mon, 03 Jun 2019 20:20:08: 16000000 INFO @ Mon, 03 Jun 2019 20:20:11: 16000000 INFO @ Mon, 03 Jun 2019 20:20:12: 20000000 INFO @ Mon, 03 Jun 2019 20:20:15: 17000000 INFO @ Mon, 03 Jun 2019 20:20:18: 21000000 INFO @ Mon, 03 Jun 2019 20:20:20: 17000000 INFO @ Mon, 03 Jun 2019 20:20:23: 18000000 INFO @ Mon, 03 Jun 2019 20:20:25: 22000000 INFO @ Mon, 03 Jun 2019 20:20:28: 18000000 INFO @ Mon, 03 Jun 2019 20:20:30: 19000000 INFO @ Mon, 03 Jun 2019 20:20:31: 23000000 INFO @ Mon, 03 Jun 2019 20:20:35: 19000000 INFO @ Mon, 03 Jun 2019 20:20:37: 24000000 INFO @ Mon, 03 Jun 2019 20:20:38: 20000000 INFO @ Mon, 03 Jun 2019 20:20:43: 20000000 INFO @ Mon, 03 Jun 2019 20:20:44: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 20:20:44: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 20:20:44: #1 total tags in treatment: 24949763 INFO @ Mon, 03 Jun 2019 20:20:44: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:20:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:20:44: #1 tags after filtering in treatment: 24949763 INFO @ Mon, 03 Jun 2019 20:20:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:20:44: #1 finished! INFO @ Mon, 03 Jun 2019 20:20:44: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:20:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:20:45: 21000000 INFO @ Mon, 03 Jun 2019 20:20:46: #2 number of paired peaks: 2 WARNING @ Mon, 03 Jun 2019 20:20:46: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:20:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:20:51: 21000000 INFO @ Mon, 03 Jun 2019 20:20:53: 22000000 INFO @ Mon, 03 Jun 2019 20:20:59: 22000000 INFO @ Mon, 03 Jun 2019 20:21:00: 23000000 INFO @ Mon, 03 Jun 2019 20:21:06: 23000000 INFO @ Mon, 03 Jun 2019 20:21:07: 24000000 INFO @ Mon, 03 Jun 2019 20:21:14: 24000000 INFO @ Mon, 03 Jun 2019 20:21:15: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 20:21:15: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 20:21:15: #1 total tags in treatment: 24949763 INFO @ Mon, 03 Jun 2019 20:21:15: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:21:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:21:15: #1 tags after filtering in treatment: 24949763 INFO @ Mon, 03 Jun 2019 20:21:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:21:15: #1 finished! INFO @ Mon, 03 Jun 2019 20:21:15: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:21:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:21:17: #2 number of paired peaks: 2 WARNING @ Mon, 03 Jun 2019 20:21:17: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:21:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:21:21: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 20:21:21: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 20:21:21: #1 total tags in treatment: 24949763 INFO @ Mon, 03 Jun 2019 20:21:21: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:21:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:21:22: #1 tags after filtering in treatment: 24949763 INFO @ Mon, 03 Jun 2019 20:21:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 20:21:22: #1 finished! INFO @ Mon, 03 Jun 2019 20:21:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:21:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:21:24: #2 number of paired peaks: 2 WARNING @ Mon, 03 Jun 2019 20:21:24: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 20:21:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX490729/SRX490729.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。