Job ID = 6528153 SRX = SRX4828017 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:31:33 prefetch.2.10.7: 1) Downloading 'SRR7997153'... 2020-06-29T14:31:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:33:16 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:33:16 prefetch.2.10.7: 'SRR7997153' is valid 2020-06-29T14:33:16 prefetch.2.10.7: 1) 'SRR7997153' was downloaded successfully 2020-06-29T14:33:16 prefetch.2.10.7: 'SRR7997153' has 0 unresolved dependencies Read 15880701 spots for SRR7997153/SRR7997153.sra Written 15880701 spots for SRR7997153/SRR7997153.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:43 15880701 reads; of these: 15880701 (100.00%) were unpaired; of these: 1211599 (7.63%) aligned 0 times 11537963 (72.65%) aligned exactly 1 time 3131139 (19.72%) aligned >1 times 92.37% overall alignment rate Time searching: 00:04:43 Overall time: 00:04:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2480976 / 14669102 = 0.1691 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:45:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:45:30: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:45:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:45:37: 1000000 INFO @ Mon, 29 Jun 2020 23:45:43: 2000000 INFO @ Mon, 29 Jun 2020 23:45:50: 3000000 INFO @ Mon, 29 Jun 2020 23:45:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:00: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:00: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:03: 5000000 INFO @ Mon, 29 Jun 2020 23:46:07: 1000000 INFO @ Mon, 29 Jun 2020 23:46:10: 6000000 INFO @ Mon, 29 Jun 2020 23:46:14: 2000000 INFO @ Mon, 29 Jun 2020 23:46:17: 7000000 INFO @ Mon, 29 Jun 2020 23:46:21: 3000000 INFO @ Mon, 29 Jun 2020 23:46:24: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:28: 4000000 INFO @ Mon, 29 Jun 2020 23:46:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:30: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:32: 9000000 INFO @ Mon, 29 Jun 2020 23:46:36: 5000000 INFO @ Mon, 29 Jun 2020 23:46:37: 1000000 INFO @ Mon, 29 Jun 2020 23:46:39: 10000000 INFO @ Mon, 29 Jun 2020 23:46:43: 6000000 INFO @ Mon, 29 Jun 2020 23:46:45: 2000000 INFO @ Mon, 29 Jun 2020 23:46:46: 11000000 INFO @ Mon, 29 Jun 2020 23:46:50: 7000000 INFO @ Mon, 29 Jun 2020 23:46:52: 3000000 INFO @ Mon, 29 Jun 2020 23:46:54: 12000000 INFO @ Mon, 29 Jun 2020 23:46:55: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 23:46:55: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 23:46:55: #1 total tags in treatment: 12188126 INFO @ Mon, 29 Jun 2020 23:46:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:46:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:46:55: #1 tags after filtering in treatment: 12188126 INFO @ Mon, 29 Jun 2020 23:46:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:46:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:46:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:46:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:46:56: #2 number of paired peaks: 92 WARNING @ Mon, 29 Jun 2020 23:46:56: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:46:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:46:58: 8000000 INFO @ Mon, 29 Jun 2020 23:46:59: 4000000 INFO @ Mon, 29 Jun 2020 23:47:05: 9000000 INFO @ Mon, 29 Jun 2020 23:47:07: 5000000 INFO @ Mon, 29 Jun 2020 23:47:13: 10000000 INFO @ Mon, 29 Jun 2020 23:47:14: 6000000 INFO @ Mon, 29 Jun 2020 23:47:20: 11000000 INFO @ Mon, 29 Jun 2020 23:47:21: 7000000 INFO @ Mon, 29 Jun 2020 23:47:28: 12000000 INFO @ Mon, 29 Jun 2020 23:47:29: 8000000 INFO @ Mon, 29 Jun 2020 23:47:29: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 23:47:29: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 23:47:29: #1 total tags in treatment: 12188126 INFO @ Mon, 29 Jun 2020 23:47:29: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:47:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:47:29: #1 tags after filtering in treatment: 12188126 INFO @ Mon, 29 Jun 2020 23:47:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:47:29: #1 finished! INFO @ Mon, 29 Jun 2020 23:47:29: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:47:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:47:30: #2 number of paired peaks: 92 WARNING @ Mon, 29 Jun 2020 23:47:30: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:47:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:47:36: 9000000 INFO @ Mon, 29 Jun 2020 23:47:42: 10000000 INFO @ Mon, 29 Jun 2020 23:47:49: 11000000 INFO @ Mon, 29 Jun 2020 23:47:56: 12000000 INFO @ Mon, 29 Jun 2020 23:47:57: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 23:47:57: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 23:47:57: #1 total tags in treatment: 12188126 INFO @ Mon, 29 Jun 2020 23:47:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:47:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:47:57: #1 tags after filtering in treatment: 12188126 INFO @ Mon, 29 Jun 2020 23:47:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:47:57: #1 finished! INFO @ Mon, 29 Jun 2020 23:47:57: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:47:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:47:58: #2 number of paired peaks: 92 WARNING @ Mon, 29 Jun 2020 23:47:58: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:47:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828017/SRX4828017.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。