Job ID = 6528152 SRX = SRX4828016 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:38:44 prefetch.2.10.7: 1) Downloading 'SRR7997152'... 2020-06-29T14:38:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:41:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:41:02 prefetch.2.10.7: 1) 'SRR7997152' was downloaded successfully 2020-06-29T14:41:02 prefetch.2.10.7: 'SRR7997152' has 0 unresolved dependencies Read 34196540 spots for SRR7997152/SRR7997152.sra Written 34196540 spots for SRR7997152/SRR7997152.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:00 34196540 reads; of these: 34196540 (100.00%) were unpaired; of these: 1177344 (3.44%) aligned 0 times 26550285 (77.64%) aligned exactly 1 time 6468911 (18.92%) aligned >1 times 96.56% overall alignment rate Time searching: 00:10:00 Overall time: 00:10:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6765464 / 33019196 = 0.2049 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:06:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:06:49: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:06:49: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:06:53: 1000000 INFO @ Tue, 30 Jun 2020 00:06:57: 2000000 INFO @ Tue, 30 Jun 2020 00:07:02: 3000000 INFO @ Tue, 30 Jun 2020 00:07:06: 4000000 INFO @ Tue, 30 Jun 2020 00:07:10: 5000000 INFO @ Tue, 30 Jun 2020 00:07:15: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:18: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:18: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:19: 7000000 INFO @ Tue, 30 Jun 2020 00:07:22: 1000000 INFO @ Tue, 30 Jun 2020 00:07:24: 8000000 INFO @ Tue, 30 Jun 2020 00:07:27: 2000000 INFO @ Tue, 30 Jun 2020 00:07:28: 9000000 INFO @ Tue, 30 Jun 2020 00:07:31: 3000000 INFO @ Tue, 30 Jun 2020 00:07:32: 10000000 INFO @ Tue, 30 Jun 2020 00:07:36: 4000000 INFO @ Tue, 30 Jun 2020 00:07:37: 11000000 INFO @ Tue, 30 Jun 2020 00:07:40: 5000000 INFO @ Tue, 30 Jun 2020 00:07:41: 12000000 INFO @ Tue, 30 Jun 2020 00:07:44: 6000000 INFO @ Tue, 30 Jun 2020 00:07:45: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:48: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:48: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:48: 7000000 INFO @ Tue, 30 Jun 2020 00:07:50: 14000000 INFO @ Tue, 30 Jun 2020 00:07:53: 1000000 INFO @ Tue, 30 Jun 2020 00:07:53: 8000000 INFO @ Tue, 30 Jun 2020 00:07:54: 15000000 INFO @ Tue, 30 Jun 2020 00:07:57: 2000000 INFO @ Tue, 30 Jun 2020 00:07:57: 9000000 INFO @ Tue, 30 Jun 2020 00:07:59: 16000000 INFO @ Tue, 30 Jun 2020 00:08:01: 3000000 INFO @ Tue, 30 Jun 2020 00:08:02: 10000000 INFO @ Tue, 30 Jun 2020 00:08:03: 17000000 INFO @ Tue, 30 Jun 2020 00:08:06: 4000000 INFO @ Tue, 30 Jun 2020 00:08:06: 11000000 INFO @ Tue, 30 Jun 2020 00:08:08: 18000000 INFO @ Tue, 30 Jun 2020 00:08:10: 5000000 INFO @ Tue, 30 Jun 2020 00:08:10: 12000000 INFO @ Tue, 30 Jun 2020 00:08:12: 19000000 INFO @ Tue, 30 Jun 2020 00:08:15: 6000000 INFO @ Tue, 30 Jun 2020 00:08:15: 13000000 INFO @ Tue, 30 Jun 2020 00:08:17: 20000000 INFO @ Tue, 30 Jun 2020 00:08:19: 7000000 INFO @ Tue, 30 Jun 2020 00:08:19: 14000000 INFO @ Tue, 30 Jun 2020 00:08:21: 21000000 INFO @ Tue, 30 Jun 2020 00:08:23: 8000000 INFO @ Tue, 30 Jun 2020 00:08:24: 15000000 INFO @ Tue, 30 Jun 2020 00:08:26: 22000000 INFO @ Tue, 30 Jun 2020 00:08:28: 9000000 INFO @ Tue, 30 Jun 2020 00:08:28: 16000000 INFO @ Tue, 30 Jun 2020 00:08:30: 23000000 INFO @ Tue, 30 Jun 2020 00:08:32: 10000000 INFO @ Tue, 30 Jun 2020 00:08:32: 17000000 INFO @ Tue, 30 Jun 2020 00:08:35: 24000000 INFO @ Tue, 30 Jun 2020 00:08:37: 11000000 INFO @ Tue, 30 Jun 2020 00:08:37: 18000000 INFO @ Tue, 30 Jun 2020 00:08:39: 25000000 INFO @ Tue, 30 Jun 2020 00:08:41: 12000000 INFO @ Tue, 30 Jun 2020 00:08:41: 19000000 INFO @ Tue, 30 Jun 2020 00:08:44: 26000000 INFO @ Tue, 30 Jun 2020 00:08:45: #1 tag size is determined as 49 bps INFO @ Tue, 30 Jun 2020 00:08:45: #1 tag size = 49 INFO @ Tue, 30 Jun 2020 00:08:45: #1 total tags in treatment: 26253732 INFO @ Tue, 30 Jun 2020 00:08:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:08:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:08:45: #1 tags after filtering in treatment: 26253732 INFO @ Tue, 30 Jun 2020 00:08:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:08:45: #1 finished! INFO @ Tue, 30 Jun 2020 00:08:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:08:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:08:46: 13000000 INFO @ Tue, 30 Jun 2020 00:08:46: 20000000 INFO @ Tue, 30 Jun 2020 00:08:47: #2 number of paired peaks: 33 WARNING @ Tue, 30 Jun 2020 00:08:47: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:08:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:08:50: 14000000 INFO @ Tue, 30 Jun 2020 00:08:50: 21000000 INFO @ Tue, 30 Jun 2020 00:08:55: 15000000 INFO @ Tue, 30 Jun 2020 00:08:55: 22000000 INFO @ Tue, 30 Jun 2020 00:08:59: 16000000 INFO @ Tue, 30 Jun 2020 00:08:59: 23000000 INFO @ Tue, 30 Jun 2020 00:09:03: 17000000 INFO @ Tue, 30 Jun 2020 00:09:04: 24000000 INFO @ Tue, 30 Jun 2020 00:09:08: 18000000 INFO @ Tue, 30 Jun 2020 00:09:08: 25000000 INFO @ Tue, 30 Jun 2020 00:09:12: 26000000 INFO @ Tue, 30 Jun 2020 00:09:12: 19000000 INFO @ Tue, 30 Jun 2020 00:09:14: #1 tag size is determined as 49 bps INFO @ Tue, 30 Jun 2020 00:09:14: #1 tag size = 49 INFO @ Tue, 30 Jun 2020 00:09:14: #1 total tags in treatment: 26253732 INFO @ Tue, 30 Jun 2020 00:09:14: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:14: #1 tags after filtering in treatment: 26253732 INFO @ Tue, 30 Jun 2020 00:09:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:14: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:14: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:16: #2 number of paired peaks: 33 WARNING @ Tue, 30 Jun 2020 00:09:16: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:09:17: 20000000 INFO @ Tue, 30 Jun 2020 00:09:21: 21000000 INFO @ Tue, 30 Jun 2020 00:09:26: 22000000 INFO @ Tue, 30 Jun 2020 00:09:30: 23000000 INFO @ Tue, 30 Jun 2020 00:09:35: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:09:39: 25000000 INFO @ Tue, 30 Jun 2020 00:09:43: 26000000 INFO @ Tue, 30 Jun 2020 00:09:45: #1 tag size is determined as 49 bps INFO @ Tue, 30 Jun 2020 00:09:45: #1 tag size = 49 INFO @ Tue, 30 Jun 2020 00:09:45: #1 total tags in treatment: 26253732 INFO @ Tue, 30 Jun 2020 00:09:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:45: #1 tags after filtering in treatment: 26253732 INFO @ Tue, 30 Jun 2020 00:09:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:45: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:47: #2 number of paired peaks: 33 WARNING @ Tue, 30 Jun 2020 00:09:47: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4828016/SRX4828016.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。