Job ID = 1299158 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-06-03T08:58:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 3,086,343 reads read : 3,086,343 reads written : 3,086,343 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:30 3086343 reads; of these: 3086343 (100.00%) were unpaired; of these: 135423 (4.39%) aligned 0 times 1992645 (64.56%) aligned exactly 1 time 958275 (31.05%) aligned >1 times 95.61% overall alignment rate Time searching: 00:01:30 Overall time: 00:01:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 70650 / 2950920 = 0.0239 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 18:03:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:03:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:03:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:03:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:03:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:03:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:03:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:03:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:03:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:03:33: 1000000 INFO @ Mon, 03 Jun 2019 18:03:35: 1000000 INFO @ Mon, 03 Jun 2019 18:03:36: 1000000 INFO @ Mon, 03 Jun 2019 18:03:40: 2000000 INFO @ Mon, 03 Jun 2019 18:03:45: 2000000 INFO @ Mon, 03 Jun 2019 18:03:46: 2000000 INFO @ Mon, 03 Jun 2019 18:03:47: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 18:03:47: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 18:03:47: #1 total tags in treatment: 2880270 INFO @ Mon, 03 Jun 2019 18:03:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:03:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:03:47: #1 tags after filtering in treatment: 2880270 INFO @ Mon, 03 Jun 2019 18:03:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:03:47: #1 finished! INFO @ Mon, 03 Jun 2019 18:03:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:03:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:03:47: #2 number of paired peaks: 633 WARNING @ Mon, 03 Jun 2019 18:03:47: Fewer paired peaks (633) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 633 pairs to build model! INFO @ Mon, 03 Jun 2019 18:03:47: start model_add_line... INFO @ Mon, 03 Jun 2019 18:03:47: start X-correlation... INFO @ Mon, 03 Jun 2019 18:03:47: end of X-cor INFO @ Mon, 03 Jun 2019 18:03:47: #2 finished! INFO @ Mon, 03 Jun 2019 18:03:47: #2 predicted fragment length is 55 bps INFO @ Mon, 03 Jun 2019 18:03:47: #2 alternative fragment length(s) may be 55 bps INFO @ Mon, 03 Jun 2019 18:03:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.10_model.r WARNING @ Mon, 03 Jun 2019 18:03:47: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 18:03:47: #2 You may need to consider one of the other alternative d(s): 55 WARNING @ Mon, 03 Jun 2019 18:03:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 18:03:47: #3 Call peaks... INFO @ Mon, 03 Jun 2019 18:03:47: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 18:03:53: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 18:03:53: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 18:03:53: #1 total tags in treatment: 2880270 INFO @ Mon, 03 Jun 2019 18:03:53: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:03:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:03:53: #1 tags after filtering in treatment: 2880270 INFO @ Mon, 03 Jun 2019 18:03:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:03:53: #1 finished! INFO @ Mon, 03 Jun 2019 18:03:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:03:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:03:54: #2 number of paired peaks: 633 WARNING @ Mon, 03 Jun 2019 18:03:54: Fewer paired peaks (633) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 633 pairs to build model! INFO @ Mon, 03 Jun 2019 18:03:54: start model_add_line... INFO @ Mon, 03 Jun 2019 18:03:54: start X-correlation... INFO @ Mon, 03 Jun 2019 18:03:54: end of X-cor INFO @ Mon, 03 Jun 2019 18:03:54: #2 finished! INFO @ Mon, 03 Jun 2019 18:03:54: #2 predicted fragment length is 55 bps INFO @ Mon, 03 Jun 2019 18:03:54: #2 alternative fragment length(s) may be 55 bps INFO @ Mon, 03 Jun 2019 18:03:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.05_model.r WARNING @ Mon, 03 Jun 2019 18:03:54: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 18:03:54: #2 You may need to consider one of the other alternative d(s): 55 WARNING @ Mon, 03 Jun 2019 18:03:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 18:03:54: #3 Call peaks... INFO @ Mon, 03 Jun 2019 18:03:54: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 18:03:54: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 18:03:54: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 18:03:54: #1 total tags in treatment: 2880270 INFO @ Mon, 03 Jun 2019 18:03:54: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:03:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:03:54: #1 tags after filtering in treatment: 2880270 INFO @ Mon, 03 Jun 2019 18:03:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:03:54: #1 finished! INFO @ Mon, 03 Jun 2019 18:03:54: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:03:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:03:55: #2 number of paired peaks: 633 WARNING @ Mon, 03 Jun 2019 18:03:55: Fewer paired peaks (633) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 633 pairs to build model! INFO @ Mon, 03 Jun 2019 18:03:55: start model_add_line... INFO @ Mon, 03 Jun 2019 18:03:55: start X-correlation... INFO @ Mon, 03 Jun 2019 18:03:55: end of X-cor INFO @ Mon, 03 Jun 2019 18:03:55: #2 finished! INFO @ Mon, 03 Jun 2019 18:03:55: #2 predicted fragment length is 55 bps INFO @ Mon, 03 Jun 2019 18:03:55: #2 alternative fragment length(s) may be 55 bps INFO @ Mon, 03 Jun 2019 18:03:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.20_model.r WARNING @ Mon, 03 Jun 2019 18:03:55: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 18:03:55: #2 You may need to consider one of the other alternative d(s): 55 WARNING @ Mon, 03 Jun 2019 18:03:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 18:03:55: #3 Call peaks... INFO @ Mon, 03 Jun 2019 18:03:55: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 18:03:56: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 18:04:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.10_peaks.xls INFO @ Mon, 03 Jun 2019 18:04:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 18:04:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.10_summits.bed INFO @ Mon, 03 Jun 2019 18:04:00: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (875 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:04:03: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 18:04:04: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 18:04:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.05_peaks.xls INFO @ Mon, 03 Jun 2019 18:04:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 18:04:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.05_summits.bed INFO @ Mon, 03 Jun 2019 18:04:07: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (1524 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:04:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.20_peaks.xls INFO @ Mon, 03 Jun 2019 18:04:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 18:04:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX474525/SRX474525.20_summits.bed INFO @ Mon, 03 Jun 2019 18:04:08: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (324 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。