Job ID = 14167143 SRX = SRX4712959 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5866739 spots for SRR7874089/SRR7874089.sra Written 5866739 spots for SRR7874089/SRR7874089.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167608 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:42 5866739 reads; of these: 5866739 (100.00%) were unpaired; of these: 117792 (2.01%) aligned 0 times 4625098 (78.84%) aligned exactly 1 time 1123849 (19.16%) aligned >1 times 97.99% overall alignment rate Time searching: 00:01:42 Overall time: 00:01:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 198721 / 5748947 = 0.0346 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:33:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:33:05: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:33:05: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:33:11: 1000000 INFO @ Fri, 10 Dec 2021 10:33:17: 2000000 INFO @ Fri, 10 Dec 2021 10:33:23: 3000000 INFO @ Fri, 10 Dec 2021 10:33:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:33:34: 5000000 INFO @ Fri, 10 Dec 2021 10:33:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:33:36: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:33:36: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:33:38: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:33:38: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:33:38: #1 total tags in treatment: 5550226 INFO @ Fri, 10 Dec 2021 10:33:38: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:33:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:33:38: #1 tags after filtering in treatment: 5550226 INFO @ Fri, 10 Dec 2021 10:33:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:33:38: #1 finished! INFO @ Fri, 10 Dec 2021 10:33:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:33:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:33:38: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 10:33:38: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:33:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:33:43: 1000000 INFO @ Fri, 10 Dec 2021 10:33:50: 2000000 INFO @ Fri, 10 Dec 2021 10:33:57: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:34:05: 4000000 INFO @ Fri, 10 Dec 2021 10:34:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:34:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:34:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:34:12: 5000000 INFO @ Fri, 10 Dec 2021 10:34:13: 1000000 INFO @ Fri, 10 Dec 2021 10:34:16: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:34:16: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:34:16: #1 total tags in treatment: 5550226 INFO @ Fri, 10 Dec 2021 10:34:16: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:34:16: #1 tags after filtering in treatment: 5550226 INFO @ Fri, 10 Dec 2021 10:34:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:34:16: #1 finished! INFO @ Fri, 10 Dec 2021 10:34:16: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:34:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:34:17: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 10:34:17: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:34:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:34:19: 2000000 INFO @ Fri, 10 Dec 2021 10:34:26: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:34:32: 4000000 INFO @ Fri, 10 Dec 2021 10:34:39: 5000000 INFO @ Fri, 10 Dec 2021 10:34:42: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:34:42: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:34:42: #1 total tags in treatment: 5550226 INFO @ Fri, 10 Dec 2021 10:34:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:34:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:34:42: #1 tags after filtering in treatment: 5550226 INFO @ Fri, 10 Dec 2021 10:34:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:34:42: #1 finished! INFO @ Fri, 10 Dec 2021 10:34:42: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:34:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:34:43: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 10:34:43: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:34:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712959/SRX4712959.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。