Job ID = 14167142 SRX = SRX4712958 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 35369724 spots for SRR7874088/SRR7874088.sra Written 35369724 spots for SRR7874088/SRR7874088.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167663 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:58 35369724 reads; of these: 35369724 (100.00%) were unpaired; of these: 2420110 (6.84%) aligned 0 times 21910892 (61.95%) aligned exactly 1 time 11038722 (31.21%) aligned >1 times 93.16% overall alignment rate Time searching: 00:14:58 Overall time: 00:14:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7267704 / 32949614 = 0.2206 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:53:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:53:00: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:53:00: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:53:06: 1000000 INFO @ Fri, 10 Dec 2021 10:53:12: 2000000 INFO @ Fri, 10 Dec 2021 10:53:18: 3000000 INFO @ Fri, 10 Dec 2021 10:53:23: 4000000 INFO @ Fri, 10 Dec 2021 10:53:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:53:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:53:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:53:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:53:34: 6000000 INFO @ Fri, 10 Dec 2021 10:53:36: 1000000 INFO @ Fri, 10 Dec 2021 10:53:40: 7000000 INFO @ Fri, 10 Dec 2021 10:53:42: 2000000 INFO @ Fri, 10 Dec 2021 10:53:46: 8000000 INFO @ Fri, 10 Dec 2021 10:53:48: 3000000 INFO @ Fri, 10 Dec 2021 10:53:52: 9000000 INFO @ Fri, 10 Dec 2021 10:53:54: 4000000 INFO @ Fri, 10 Dec 2021 10:53:58: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:54:00: 5000000 INFO @ Fri, 10 Dec 2021 10:54:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:54:00: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:54:00: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:54:04: 11000000 INFO @ Fri, 10 Dec 2021 10:54:07: 6000000 INFO @ Fri, 10 Dec 2021 10:54:07: 1000000 INFO @ Fri, 10 Dec 2021 10:54:10: 12000000 INFO @ Fri, 10 Dec 2021 10:54:13: 7000000 INFO @ Fri, 10 Dec 2021 10:54:13: 2000000 INFO @ Fri, 10 Dec 2021 10:54:17: 13000000 INFO @ Fri, 10 Dec 2021 10:54:19: 8000000 INFO @ Fri, 10 Dec 2021 10:54:20: 3000000 INFO @ Fri, 10 Dec 2021 10:54:23: 14000000 INFO @ Fri, 10 Dec 2021 10:54:25: 9000000 INFO @ Fri, 10 Dec 2021 10:54:26: 4000000 INFO @ Fri, 10 Dec 2021 10:54:29: 15000000 INFO @ Fri, 10 Dec 2021 10:54:32: 10000000 INFO @ Fri, 10 Dec 2021 10:54:32: 5000000 INFO @ Fri, 10 Dec 2021 10:54:35: 16000000 INFO @ Fri, 10 Dec 2021 10:54:38: 11000000 INFO @ Fri, 10 Dec 2021 10:54:38: 6000000 INFO @ Fri, 10 Dec 2021 10:54:42: 17000000 INFO @ Fri, 10 Dec 2021 10:54:44: 12000000 INFO @ Fri, 10 Dec 2021 10:54:45: 7000000 INFO @ Fri, 10 Dec 2021 10:54:48: 18000000 INFO @ Fri, 10 Dec 2021 10:54:50: 13000000 INFO @ Fri, 10 Dec 2021 10:54:51: 8000000 INFO @ Fri, 10 Dec 2021 10:54:54: 19000000 INFO @ Fri, 10 Dec 2021 10:54:57: 14000000 INFO @ Fri, 10 Dec 2021 10:54:57: 9000000 INFO @ Fri, 10 Dec 2021 10:55:01: 20000000 INFO @ Fri, 10 Dec 2021 10:55:03: 15000000 INFO @ Fri, 10 Dec 2021 10:55:04: 10000000 INFO @ Fri, 10 Dec 2021 10:55:08: 21000000 INFO @ Fri, 10 Dec 2021 10:55:10: 16000000 INFO @ Fri, 10 Dec 2021 10:55:10: 11000000 INFO @ Fri, 10 Dec 2021 10:55:14: 22000000 INFO @ Fri, 10 Dec 2021 10:55:16: 17000000 INFO @ Fri, 10 Dec 2021 10:55:16: 12000000 INFO @ Fri, 10 Dec 2021 10:55:20: 23000000 INFO @ Fri, 10 Dec 2021 10:55:22: 18000000 INFO @ Fri, 10 Dec 2021 10:55:23: 13000000 INFO @ Fri, 10 Dec 2021 10:55:27: 24000000 INFO @ Fri, 10 Dec 2021 10:55:29: 19000000 INFO @ Fri, 10 Dec 2021 10:55:29: 14000000 INFO @ Fri, 10 Dec 2021 10:55:33: 25000000 INFO @ Fri, 10 Dec 2021 10:55:35: 20000000 INFO @ Fri, 10 Dec 2021 10:55:35: 15000000 INFO @ Fri, 10 Dec 2021 10:55:37: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:55:37: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:55:37: #1 total tags in treatment: 25681910 INFO @ Fri, 10 Dec 2021 10:55:37: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:55:38: #1 tags after filtering in treatment: 25681910 INFO @ Fri, 10 Dec 2021 10:55:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:55:38: #1 finished! INFO @ Fri, 10 Dec 2021 10:55:38: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:55:39: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 10:55:39: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:55:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:55:41: 21000000 INFO @ Fri, 10 Dec 2021 10:55:41: 16000000 INFO @ Fri, 10 Dec 2021 10:55:47: 22000000 INFO @ Fri, 10 Dec 2021 10:55:47: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:55:54: 23000000 INFO @ Fri, 10 Dec 2021 10:55:54: 18000000 INFO @ Fri, 10 Dec 2021 10:56:00: 24000000 INFO @ Fri, 10 Dec 2021 10:56:00: 19000000 INFO @ Fri, 10 Dec 2021 10:56:06: 25000000 INFO @ Fri, 10 Dec 2021 10:56:06: 20000000 INFO @ Fri, 10 Dec 2021 10:56:10: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:56:10: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:56:10: #1 total tags in treatment: 25681910 INFO @ Fri, 10 Dec 2021 10:56:10: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:56:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:56:11: #1 tags after filtering in treatment: 25681910 INFO @ Fri, 10 Dec 2021 10:56:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:56:11: #1 finished! INFO @ Fri, 10 Dec 2021 10:56:11: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:56:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:56:12: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 10:56:12: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:56:12: Process for pairing-model is terminated! INFO @ Fri, 10 Dec 2021 10:56:12: 21000000 cut: /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:56:18: 22000000 INFO @ Fri, 10 Dec 2021 10:56:24: 23000000 INFO @ Fri, 10 Dec 2021 10:56:30: 24000000 INFO @ Fri, 10 Dec 2021 10:56:35: 25000000 INFO @ Fri, 10 Dec 2021 10:56:39: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:56:39: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:56:39: #1 total tags in treatment: 25681910 INFO @ Fri, 10 Dec 2021 10:56:39: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:56:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:56:40: #1 tags after filtering in treatment: 25681910 INFO @ Fri, 10 Dec 2021 10:56:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:56:40: #1 finished! INFO @ Fri, 10 Dec 2021 10:56:40: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:56:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:56:41: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 10:56:41: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:56:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712958/SRX4712958.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。