Job ID = 14167141 SRX = SRX4712957 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 30167676 spots for SRR7874087/SRR7874087.sra Written 30167676 spots for SRR7874087/SRR7874087.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167649 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:44 30167676 reads; of these: 30167676 (100.00%) were unpaired; of these: 675658 (2.24%) aligned 0 times 21958723 (72.79%) aligned exactly 1 time 7533295 (24.97%) aligned >1 times 97.76% overall alignment rate Time searching: 00:09:44 Overall time: 00:09:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3790164 / 29492018 = 0.1285 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:47:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:47:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:47:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:47:26: 1000000 INFO @ Fri, 10 Dec 2021 10:47:31: 2000000 INFO @ Fri, 10 Dec 2021 10:47:36: 3000000 INFO @ Fri, 10 Dec 2021 10:47:41: 4000000 INFO @ Fri, 10 Dec 2021 10:47:46: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:47:51: 6000000 INFO @ Fri, 10 Dec 2021 10:47:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:47:51: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:47:51: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:47:56: 7000000 INFO @ Fri, 10 Dec 2021 10:47:56: 1000000 INFO @ Fri, 10 Dec 2021 10:48:01: 8000000 INFO @ Fri, 10 Dec 2021 10:48:01: 2000000 INFO @ Fri, 10 Dec 2021 10:48:06: 9000000 INFO @ Fri, 10 Dec 2021 10:48:06: 3000000 INFO @ Fri, 10 Dec 2021 10:48:11: 10000000 INFO @ Fri, 10 Dec 2021 10:48:12: 4000000 INFO @ Fri, 10 Dec 2021 10:48:16: 11000000 INFO @ Fri, 10 Dec 2021 10:48:17: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:48:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:48:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:48:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:48:22: 12000000 INFO @ Fri, 10 Dec 2021 10:48:22: 6000000 INFO @ Fri, 10 Dec 2021 10:48:27: 13000000 INFO @ Fri, 10 Dec 2021 10:48:27: 1000000 INFO @ Fri, 10 Dec 2021 10:48:27: 7000000 INFO @ Fri, 10 Dec 2021 10:48:32: 14000000 INFO @ Fri, 10 Dec 2021 10:48:33: 8000000 INFO @ Fri, 10 Dec 2021 10:48:33: 2000000 INFO @ Fri, 10 Dec 2021 10:48:38: 15000000 INFO @ Fri, 10 Dec 2021 10:48:38: 9000000 INFO @ Fri, 10 Dec 2021 10:48:39: 3000000 INFO @ Fri, 10 Dec 2021 10:48:43: 16000000 INFO @ Fri, 10 Dec 2021 10:48:43: 10000000 INFO @ Fri, 10 Dec 2021 10:48:45: 4000000 INFO @ Fri, 10 Dec 2021 10:48:48: 17000000 INFO @ Fri, 10 Dec 2021 10:48:49: 11000000 INFO @ Fri, 10 Dec 2021 10:48:51: 5000000 INFO @ Fri, 10 Dec 2021 10:48:54: 18000000 INFO @ Fri, 10 Dec 2021 10:48:54: 12000000 INFO @ Fri, 10 Dec 2021 10:48:58: 6000000 INFO @ Fri, 10 Dec 2021 10:48:59: 19000000 INFO @ Fri, 10 Dec 2021 10:49:00: 13000000 INFO @ Fri, 10 Dec 2021 10:49:04: 7000000 INFO @ Fri, 10 Dec 2021 10:49:05: 20000000 INFO @ Fri, 10 Dec 2021 10:49:05: 14000000 INFO @ Fri, 10 Dec 2021 10:49:10: 8000000 INFO @ Fri, 10 Dec 2021 10:49:10: 21000000 INFO @ Fri, 10 Dec 2021 10:49:11: 15000000 INFO @ Fri, 10 Dec 2021 10:49:16: 22000000 INFO @ Fri, 10 Dec 2021 10:49:16: 9000000 INFO @ Fri, 10 Dec 2021 10:49:16: 16000000 INFO @ Fri, 10 Dec 2021 10:49:21: 23000000 INFO @ Fri, 10 Dec 2021 10:49:21: 17000000 INFO @ Fri, 10 Dec 2021 10:49:22: 10000000 INFO @ Fri, 10 Dec 2021 10:49:26: 24000000 INFO @ Fri, 10 Dec 2021 10:49:27: 18000000 INFO @ Fri, 10 Dec 2021 10:49:28: 11000000 INFO @ Fri, 10 Dec 2021 10:49:32: 25000000 INFO @ Fri, 10 Dec 2021 10:49:32: 19000000 INFO @ Fri, 10 Dec 2021 10:49:34: 12000000 INFO @ Fri, 10 Dec 2021 10:49:36: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:49:36: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:49:36: #1 total tags in treatment: 25701854 INFO @ Fri, 10 Dec 2021 10:49:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:49:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:49:36: #1 tags after filtering in treatment: 25701854 INFO @ Fri, 10 Dec 2021 10:49:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:49:36: #1 finished! INFO @ Fri, 10 Dec 2021 10:49:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:49:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:49:37: 20000000 INFO @ Fri, 10 Dec 2021 10:49:38: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 10:49:38: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:49:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:49:40: 13000000 INFO @ Fri, 10 Dec 2021 10:49:43: 21000000 INFO @ Fri, 10 Dec 2021 10:49:46: 14000000 INFO @ Fri, 10 Dec 2021 10:49:48: 22000000 INFO @ Fri, 10 Dec 2021 10:49:52: 15000000 INFO @ Fri, 10 Dec 2021 10:49:54: 23000000 INFO @ Fri, 10 Dec 2021 10:49:58: 16000000 INFO @ Fri, 10 Dec 2021 10:49:59: 24000000 INFO @ Fri, 10 Dec 2021 10:50:04: 17000000 INFO @ Fri, 10 Dec 2021 10:50:04: 25000000 INFO @ Fri, 10 Dec 2021 10:50:08: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:50:08: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:50:08: #1 total tags in treatment: 25701854 INFO @ Fri, 10 Dec 2021 10:50:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:50:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:50:09: #1 tags after filtering in treatment: 25701854 INFO @ Fri, 10 Dec 2021 10:50:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:50:09: #1 finished! INFO @ Fri, 10 Dec 2021 10:50:09: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:50:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:50:10: 18000000 INFO @ Fri, 10 Dec 2021 10:50:10: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 10:50:10: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:50:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:50:16: 19000000 INFO @ Fri, 10 Dec 2021 10:50:22: 20000000 INFO @ Fri, 10 Dec 2021 10:50:28: 21000000 INFO @ Fri, 10 Dec 2021 10:50:34: 22000000 INFO @ Fri, 10 Dec 2021 10:50:40: 23000000 INFO @ Fri, 10 Dec 2021 10:50:46: 24000000 INFO @ Fri, 10 Dec 2021 10:50:52: 25000000 INFO @ Fri, 10 Dec 2021 10:50:57: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:50:57: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:50:57: #1 total tags in treatment: 25701854 INFO @ Fri, 10 Dec 2021 10:50:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:50:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:50:57: #1 tags after filtering in treatment: 25701854 INFO @ Fri, 10 Dec 2021 10:50:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:50:57: #1 finished! INFO @ Fri, 10 Dec 2021 10:50:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:50:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:50:59: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 10:50:59: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:50:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712957/SRX4712957.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。