Job ID = 14167119 SRX = SRX4712941 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26357554 spots for SRR7874071/SRR7874071.sra Written 26357554 spots for SRR7874071/SRR7874071.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167559 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:27 26357554 reads; of these: 26357554 (100.00%) were unpaired; of these: 527821 (2.00%) aligned 0 times 18329716 (69.54%) aligned exactly 1 time 7500017 (28.45%) aligned >1 times 98.00% overall alignment rate Time searching: 00:09:28 Overall time: 00:09:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4754199 / 25829733 = 0.1841 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:20:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:20:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:20:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:20:21: 1000000 INFO @ Fri, 10 Dec 2021 10:20:26: 2000000 INFO @ Fri, 10 Dec 2021 10:20:31: 3000000 INFO @ Fri, 10 Dec 2021 10:20:36: 4000000 INFO @ Fri, 10 Dec 2021 10:20:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:20:46: 6000000 INFO @ Fri, 10 Dec 2021 10:20:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:20:46: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:20:46: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:20:52: 7000000 INFO @ Fri, 10 Dec 2021 10:20:52: 1000000 INFO @ Fri, 10 Dec 2021 10:20:57: 8000000 INFO @ Fri, 10 Dec 2021 10:20:57: 2000000 INFO @ Fri, 10 Dec 2021 10:21:02: 9000000 INFO @ Fri, 10 Dec 2021 10:21:02: 3000000 INFO @ Fri, 10 Dec 2021 10:21:07: 10000000 INFO @ Fri, 10 Dec 2021 10:21:07: 4000000 INFO @ Fri, 10 Dec 2021 10:21:13: 11000000 INFO @ Fri, 10 Dec 2021 10:21:13: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:21:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:21:16: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:21:16: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:21:18: 12000000 INFO @ Fri, 10 Dec 2021 10:21:18: 6000000 INFO @ Fri, 10 Dec 2021 10:21:23: 1000000 INFO @ Fri, 10 Dec 2021 10:21:23: 13000000 INFO @ Fri, 10 Dec 2021 10:21:24: 7000000 INFO @ Fri, 10 Dec 2021 10:21:29: 2000000 INFO @ Fri, 10 Dec 2021 10:21:29: 14000000 INFO @ Fri, 10 Dec 2021 10:21:29: 8000000 INFO @ Fri, 10 Dec 2021 10:21:34: 15000000 INFO @ Fri, 10 Dec 2021 10:21:35: 9000000 INFO @ Fri, 10 Dec 2021 10:21:35: 3000000 INFO @ Fri, 10 Dec 2021 10:21:40: 16000000 INFO @ Fri, 10 Dec 2021 10:21:40: 10000000 INFO @ Fri, 10 Dec 2021 10:21:41: 4000000 INFO @ Fri, 10 Dec 2021 10:21:46: 17000000 INFO @ Fri, 10 Dec 2021 10:21:46: 11000000 INFO @ Fri, 10 Dec 2021 10:21:48: 5000000 INFO @ Fri, 10 Dec 2021 10:21:51: 12000000 INFO @ Fri, 10 Dec 2021 10:21:51: 18000000 INFO @ Fri, 10 Dec 2021 10:21:54: 6000000 INFO @ Fri, 10 Dec 2021 10:21:57: 13000000 INFO @ Fri, 10 Dec 2021 10:21:57: 19000000 INFO @ Fri, 10 Dec 2021 10:22:00: 7000000 INFO @ Fri, 10 Dec 2021 10:22:02: 14000000 INFO @ Fri, 10 Dec 2021 10:22:02: 20000000 INFO @ Fri, 10 Dec 2021 10:22:06: 8000000 INFO @ Fri, 10 Dec 2021 10:22:08: 15000000 INFO @ Fri, 10 Dec 2021 10:22:08: 21000000 INFO @ Fri, 10 Dec 2021 10:22:08: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:22:08: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:22:08: #1 total tags in treatment: 21075534 INFO @ Fri, 10 Dec 2021 10:22:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:22:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:22:09: #1 tags after filtering in treatment: 21075534 INFO @ Fri, 10 Dec 2021 10:22:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:22:09: #1 finished! INFO @ Fri, 10 Dec 2021 10:22:09: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:22:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:22:10: #2 number of paired peaks: 64 WARNING @ Fri, 10 Dec 2021 10:22:10: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:22:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:22:12: 9000000 INFO @ Fri, 10 Dec 2021 10:22:13: 16000000 INFO @ Fri, 10 Dec 2021 10:22:19: 10000000 INFO @ Fri, 10 Dec 2021 10:22:19: 17000000 INFO @ Fri, 10 Dec 2021 10:22:24: 18000000 INFO @ Fri, 10 Dec 2021 10:22:25: 11000000 INFO @ Fri, 10 Dec 2021 10:22:30: 19000000 INFO @ Fri, 10 Dec 2021 10:22:31: 12000000 INFO @ Fri, 10 Dec 2021 10:22:35: 20000000 INFO @ Fri, 10 Dec 2021 10:22:37: 13000000 INFO @ Fri, 10 Dec 2021 10:22:41: 21000000 INFO @ Fri, 10 Dec 2021 10:22:41: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:22:41: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:22:41: #1 total tags in treatment: 21075534 INFO @ Fri, 10 Dec 2021 10:22:41: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:22:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:22:42: #1 tags after filtering in treatment: 21075534 INFO @ Fri, 10 Dec 2021 10:22:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:22:42: #1 finished! INFO @ Fri, 10 Dec 2021 10:22:42: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:22:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:22:43: #2 number of paired peaks: 64 WARNING @ Fri, 10 Dec 2021 10:22:43: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:22:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:22:44: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:22:49: 15000000 INFO @ Fri, 10 Dec 2021 10:22:55: 16000000 INFO @ Fri, 10 Dec 2021 10:23:01: 17000000 INFO @ Fri, 10 Dec 2021 10:23:07: 18000000 INFO @ Fri, 10 Dec 2021 10:23:13: 19000000 INFO @ Fri, 10 Dec 2021 10:23:19: 20000000 INFO @ Fri, 10 Dec 2021 10:23:25: 21000000 INFO @ Fri, 10 Dec 2021 10:23:26: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:23:26: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:23:26: #1 total tags in treatment: 21075534 INFO @ Fri, 10 Dec 2021 10:23:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:23:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:23:26: #1 tags after filtering in treatment: 21075534 INFO @ Fri, 10 Dec 2021 10:23:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:23:26: #1 finished! INFO @ Fri, 10 Dec 2021 10:23:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:23:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:23:27: #2 number of paired peaks: 64 WARNING @ Fri, 10 Dec 2021 10:23:27: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:23:27: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712941/SRX4712941.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling