Job ID = 14167118 SRX = SRX4712940 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13123785 spots for SRR7874070/SRR7874070.sra Written 13123785 spots for SRR7874070/SRR7874070.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167539 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:16 13123785 reads; of these: 13123785 (100.00%) were unpaired; of these: 526689 (4.01%) aligned 0 times 9413998 (71.73%) aligned exactly 1 time 3183098 (24.25%) aligned >1 times 95.99% overall alignment rate Time searching: 00:04:16 Overall time: 00:04:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1215932 / 12597096 = 0.0965 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:10:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:10:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:10:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:11:00: 1000000 INFO @ Fri, 10 Dec 2021 10:11:05: 2000000 INFO @ Fri, 10 Dec 2021 10:11:11: 3000000 INFO @ Fri, 10 Dec 2021 10:11:16: 4000000 INFO @ Fri, 10 Dec 2021 10:11:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:11:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:11:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:11:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:11:27: 6000000 INFO @ Fri, 10 Dec 2021 10:11:32: 1000000 INFO @ Fri, 10 Dec 2021 10:11:34: 7000000 INFO @ Fri, 10 Dec 2021 10:11:39: 2000000 INFO @ Fri, 10 Dec 2021 10:11:40: 8000000 INFO @ Fri, 10 Dec 2021 10:11:46: 3000000 INFO @ Fri, 10 Dec 2021 10:11:47: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:11:53: 4000000 INFO @ Fri, 10 Dec 2021 10:11:53: 10000000 INFO @ Fri, 10 Dec 2021 10:11:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:11:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:11:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:12:00: 11000000 INFO @ Fri, 10 Dec 2021 10:12:00: 5000000 INFO @ Fri, 10 Dec 2021 10:12:01: 1000000 INFO @ Fri, 10 Dec 2021 10:12:02: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:12:02: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:12:02: #1 total tags in treatment: 11381164 INFO @ Fri, 10 Dec 2021 10:12:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:12:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:12:03: #1 tags after filtering in treatment: 11381164 INFO @ Fri, 10 Dec 2021 10:12:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:12:03: #1 finished! INFO @ Fri, 10 Dec 2021 10:12:03: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:12:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:12:03: #2 number of paired peaks: 93 WARNING @ Fri, 10 Dec 2021 10:12:03: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:12:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:12:07: 6000000 INFO @ Fri, 10 Dec 2021 10:12:08: 2000000 INFO @ Fri, 10 Dec 2021 10:12:14: 7000000 INFO @ Fri, 10 Dec 2021 10:12:14: 3000000 INFO @ Fri, 10 Dec 2021 10:12:20: 4000000 INFO @ Fri, 10 Dec 2021 10:12:20: 8000000 INFO @ Fri, 10 Dec 2021 10:12:27: 5000000 INFO @ Fri, 10 Dec 2021 10:12:27: 9000000 INFO @ Fri, 10 Dec 2021 10:12:33: 6000000 INFO @ Fri, 10 Dec 2021 10:12:34: 10000000 INFO @ Fri, 10 Dec 2021 10:12:39: 7000000 INFO @ Fri, 10 Dec 2021 10:12:41: 11000000 INFO @ Fri, 10 Dec 2021 10:12:43: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:12:43: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:12:43: #1 total tags in treatment: 11381164 INFO @ Fri, 10 Dec 2021 10:12:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:12:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:12:43: #1 tags after filtering in treatment: 11381164 INFO @ Fri, 10 Dec 2021 10:12:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:12:43: #1 finished! INFO @ Fri, 10 Dec 2021 10:12:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:12:43: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:12:44: #2 number of paired peaks: 93 WARNING @ Fri, 10 Dec 2021 10:12:44: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:12:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:12:45: 8000000 INFO @ Fri, 10 Dec 2021 10:12:51: 9000000 INFO @ Fri, 10 Dec 2021 10:12:56: 10000000 INFO @ Fri, 10 Dec 2021 10:13:02: 11000000 INFO @ Fri, 10 Dec 2021 10:13:04: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:13:04: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:13:04: #1 total tags in treatment: 11381164 INFO @ Fri, 10 Dec 2021 10:13:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:13:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:13:04: #1 tags after filtering in treatment: 11381164 INFO @ Fri, 10 Dec 2021 10:13:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:13:04: #1 finished! INFO @ Fri, 10 Dec 2021 10:13:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:13:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:13:04: #2 number of paired peaks: 93 WARNING @ Fri, 10 Dec 2021 10:13:04: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:13:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712940/SRX4712940.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。