Job ID = 14167087 SRX = SRX4712929 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 50996861 spots for SRR7874059/SRR7874059.sra Written 50996861 spots for SRR7874059/SRR7874059.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167824 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:32:55 50996861 reads; of these: 50996861 (100.00%) were paired; of these: 20780757 (40.75%) aligned concordantly 0 times 22297031 (43.72%) aligned concordantly exactly 1 time 7919073 (15.53%) aligned concordantly >1 times ---- 20780757 pairs aligned concordantly 0 times; of these: 4259426 (20.50%) aligned discordantly 1 time ---- 16521331 pairs aligned 0 times concordantly or discordantly; of these: 33042662 mates make up the pairs; of these: 28737728 (86.97%) aligned 0 times 1345517 (4.07%) aligned exactly 1 time 2959417 (8.96%) aligned >1 times 71.82% overall alignment rate Time searching: 01:32:55 Overall time: 01:32:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8042116 / 34161508 = 0.2354 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:37:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:37:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:37:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:37:47: 1000000 INFO @ Fri, 10 Dec 2021 11:37:52: 2000000 INFO @ Fri, 10 Dec 2021 11:37:57: 3000000 INFO @ Fri, 10 Dec 2021 11:38:02: 4000000 INFO @ Fri, 10 Dec 2021 11:38:07: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:38:12: 6000000 INFO @ Fri, 10 Dec 2021 11:38:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:38:12: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:38:12: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:38:17: 7000000 INFO @ Fri, 10 Dec 2021 11:38:19: 1000000 INFO @ Fri, 10 Dec 2021 11:38:23: 8000000 INFO @ Fri, 10 Dec 2021 11:38:26: 2000000 INFO @ Fri, 10 Dec 2021 11:38:28: 9000000 INFO @ Fri, 10 Dec 2021 11:38:32: 3000000 INFO @ Fri, 10 Dec 2021 11:38:34: 10000000 INFO @ Fri, 10 Dec 2021 11:38:39: 4000000 INFO @ Fri, 10 Dec 2021 11:38:39: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:38:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:38:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:38:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:38:45: 12000000 INFO @ Fri, 10 Dec 2021 11:38:46: 5000000 INFO @ Fri, 10 Dec 2021 11:38:48: 1000000 INFO @ Fri, 10 Dec 2021 11:38:50: 13000000 INFO @ Fri, 10 Dec 2021 11:38:52: 6000000 INFO @ Fri, 10 Dec 2021 11:38:53: 2000000 INFO @ Fri, 10 Dec 2021 11:38:56: 14000000 INFO @ Fri, 10 Dec 2021 11:38:59: 7000000 INFO @ Fri, 10 Dec 2021 11:38:59: 3000000 INFO @ Fri, 10 Dec 2021 11:39:02: 15000000 INFO @ Fri, 10 Dec 2021 11:39:05: 4000000 INFO @ Fri, 10 Dec 2021 11:39:06: 8000000 INFO @ Fri, 10 Dec 2021 11:39:07: 16000000 INFO @ Fri, 10 Dec 2021 11:39:10: 5000000 INFO @ Fri, 10 Dec 2021 11:39:13: 9000000 INFO @ Fri, 10 Dec 2021 11:39:13: 17000000 INFO @ Fri, 10 Dec 2021 11:39:16: 6000000 INFO @ Fri, 10 Dec 2021 11:39:18: 18000000 INFO @ Fri, 10 Dec 2021 11:39:19: 10000000 INFO @ Fri, 10 Dec 2021 11:39:21: 7000000 INFO @ Fri, 10 Dec 2021 11:39:24: 19000000 INFO @ Fri, 10 Dec 2021 11:39:26: 11000000 INFO @ Fri, 10 Dec 2021 11:39:27: 8000000 INFO @ Fri, 10 Dec 2021 11:39:29: 20000000 INFO @ Fri, 10 Dec 2021 11:39:33: 9000000 INFO @ Fri, 10 Dec 2021 11:39:33: 12000000 INFO @ Fri, 10 Dec 2021 11:39:35: 21000000 INFO @ Fri, 10 Dec 2021 11:39:38: 10000000 INFO @ Fri, 10 Dec 2021 11:39:39: 13000000 INFO @ Fri, 10 Dec 2021 11:39:40: 22000000 INFO @ Fri, 10 Dec 2021 11:39:44: 11000000 INFO @ Fri, 10 Dec 2021 11:39:45: 23000000 INFO @ Fri, 10 Dec 2021 11:39:46: 14000000 INFO @ Fri, 10 Dec 2021 11:39:49: 12000000 INFO @ Fri, 10 Dec 2021 11:39:51: 24000000 INFO @ Fri, 10 Dec 2021 11:39:53: 15000000 INFO @ Fri, 10 Dec 2021 11:39:55: 13000000 INFO @ Fri, 10 Dec 2021 11:39:56: 25000000 INFO @ Fri, 10 Dec 2021 11:39:59: 16000000 INFO @ Fri, 10 Dec 2021 11:40:00: 14000000 INFO @ Fri, 10 Dec 2021 11:40:02: 26000000 INFO @ Fri, 10 Dec 2021 11:40:06: 15000000 INFO @ Fri, 10 Dec 2021 11:40:06: 17000000 INFO @ Fri, 10 Dec 2021 11:40:07: 27000000 INFO @ Fri, 10 Dec 2021 11:40:11: 16000000 INFO @ Fri, 10 Dec 2021 11:40:12: 18000000 INFO @ Fri, 10 Dec 2021 11:40:13: 28000000 INFO @ Fri, 10 Dec 2021 11:40:17: 17000000 INFO @ Fri, 10 Dec 2021 11:40:18: 29000000 INFO @ Fri, 10 Dec 2021 11:40:19: 19000000 INFO @ Fri, 10 Dec 2021 11:40:22: 18000000 INFO @ Fri, 10 Dec 2021 11:40:23: 30000000 INFO @ Fri, 10 Dec 2021 11:40:25: 20000000 INFO @ Fri, 10 Dec 2021 11:40:28: 19000000 INFO @ Fri, 10 Dec 2021 11:40:29: 31000000 INFO @ Fri, 10 Dec 2021 11:40:31: 21000000 INFO @ Fri, 10 Dec 2021 11:40:33: 20000000 INFO @ Fri, 10 Dec 2021 11:40:34: 32000000 INFO @ Fri, 10 Dec 2021 11:40:37: 22000000 INFO @ Fri, 10 Dec 2021 11:40:39: 21000000 INFO @ Fri, 10 Dec 2021 11:40:40: 33000000 INFO @ Fri, 10 Dec 2021 11:40:43: 23000000 INFO @ Fri, 10 Dec 2021 11:40:44: 22000000 INFO @ Fri, 10 Dec 2021 11:40:45: 34000000 INFO @ Fri, 10 Dec 2021 11:40:50: 24000000 INFO @ Fri, 10 Dec 2021 11:40:50: 23000000 INFO @ Fri, 10 Dec 2021 11:40:51: 35000000 INFO @ Fri, 10 Dec 2021 11:40:55: 24000000 INFO @ Fri, 10 Dec 2021 11:40:56: 25000000 INFO @ Fri, 10 Dec 2021 11:40:56: 36000000 INFO @ Fri, 10 Dec 2021 11:41:01: 25000000 INFO @ Fri, 10 Dec 2021 11:41:02: 37000000 INFO @ Fri, 10 Dec 2021 11:41:02: 26000000 INFO @ Fri, 10 Dec 2021 11:41:06: 26000000 INFO @ Fri, 10 Dec 2021 11:41:07: 38000000 INFO @ Fri, 10 Dec 2021 11:41:08: 27000000 INFO @ Fri, 10 Dec 2021 11:41:12: 27000000 INFO @ Fri, 10 Dec 2021 11:41:12: 39000000 INFO @ Fri, 10 Dec 2021 11:41:15: 28000000 INFO @ Fri, 10 Dec 2021 11:41:17: 28000000 INFO @ Fri, 10 Dec 2021 11:41:18: 40000000 INFO @ Fri, 10 Dec 2021 11:41:21: 29000000 INFO @ Fri, 10 Dec 2021 11:41:23: 29000000 INFO @ Fri, 10 Dec 2021 11:41:23: 41000000 INFO @ Fri, 10 Dec 2021 11:41:27: 30000000 INFO @ Fri, 10 Dec 2021 11:41:28: 30000000 INFO @ Fri, 10 Dec 2021 11:41:29: 42000000 INFO @ Fri, 10 Dec 2021 11:41:33: 31000000 INFO @ Fri, 10 Dec 2021 11:41:34: 31000000 INFO @ Fri, 10 Dec 2021 11:41:34: 43000000 INFO @ Fri, 10 Dec 2021 11:41:39: 32000000 INFO @ Fri, 10 Dec 2021 11:41:40: 32000000 INFO @ Fri, 10 Dec 2021 11:41:40: 44000000 INFO @ Fri, 10 Dec 2021 11:41:46: 33000000 INFO @ Fri, 10 Dec 2021 11:41:46: 45000000 INFO @ Fri, 10 Dec 2021 11:41:46: 33000000 INFO @ Fri, 10 Dec 2021 11:41:51: 46000000 INFO @ Fri, 10 Dec 2021 11:41:52: 34000000 INFO @ Fri, 10 Dec 2021 11:41:53: 34000000 INFO @ Fri, 10 Dec 2021 11:41:57: 47000000 INFO @ Fri, 10 Dec 2021 11:41:58: 35000000 INFO @ Fri, 10 Dec 2021 11:41:59: 35000000 INFO @ Fri, 10 Dec 2021 11:42:02: 48000000 INFO @ Fri, 10 Dec 2021 11:42:04: 36000000 INFO @ Fri, 10 Dec 2021 11:42:05: 36000000 INFO @ Fri, 10 Dec 2021 11:42:08: 49000000 INFO @ Fri, 10 Dec 2021 11:42:11: 37000000 INFO @ Fri, 10 Dec 2021 11:42:11: 37000000 INFO @ Fri, 10 Dec 2021 11:42:14: 50000000 INFO @ Fri, 10 Dec 2021 11:42:17: 38000000 INFO @ Fri, 10 Dec 2021 11:42:18: 38000000 INFO @ Fri, 10 Dec 2021 11:42:19: 51000000 INFO @ Fri, 10 Dec 2021 11:42:23: 39000000 INFO @ Fri, 10 Dec 2021 11:42:24: 39000000 INFO @ Fri, 10 Dec 2021 11:42:25: 52000000 INFO @ Fri, 10 Dec 2021 11:42:30: 40000000 INFO @ Fri, 10 Dec 2021 11:42:30: 53000000 INFO @ Fri, 10 Dec 2021 11:42:30: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:42:36: 54000000 INFO @ Fri, 10 Dec 2021 11:42:36: 41000000 INFO @ Fri, 10 Dec 2021 11:42:37: 41000000 INFO @ Fri, 10 Dec 2021 11:42:41: 55000000 INFO @ Fri, 10 Dec 2021 11:42:43: 42000000 INFO @ Fri, 10 Dec 2021 11:42:43: 42000000 INFO @ Fri, 10 Dec 2021 11:42:47: 56000000 INFO @ Fri, 10 Dec 2021 11:42:49: 43000000 INFO @ Fri, 10 Dec 2021 11:42:50: 43000000 INFO @ Fri, 10 Dec 2021 11:42:52: 57000000 INFO @ Fri, 10 Dec 2021 11:42:53: #1 tag size is determined as 76 bps INFO @ Fri, 10 Dec 2021 11:42:53: #1 tag size = 76 INFO @ Fri, 10 Dec 2021 11:42:53: #1 total tags in treatment: 23116552 INFO @ Fri, 10 Dec 2021 11:42:53: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:42:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:42:54: #1 tags after filtering in treatment: 18646867 INFO @ Fri, 10 Dec 2021 11:42:54: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 10 Dec 2021 11:42:54: #1 finished! INFO @ Fri, 10 Dec 2021 11:42:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:42:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:42:55: #2 number of paired peaks: 56 WARNING @ Fri, 10 Dec 2021 11:42:55: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:42:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:42:56: 44000000 INFO @ Fri, 10 Dec 2021 11:42:57: 44000000 INFO @ Fri, 10 Dec 2021 11:43:03: 45000000 INFO @ Fri, 10 Dec 2021 11:43:04: 45000000 INFO @ Fri, 10 Dec 2021 11:43:10: 46000000 INFO @ Fri, 10 Dec 2021 11:43:11: 46000000 INFO @ Fri, 10 Dec 2021 11:43:17: 47000000 INFO @ Fri, 10 Dec 2021 11:43:17: 47000000 INFO @ Fri, 10 Dec 2021 11:43:23: 48000000 INFO @ Fri, 10 Dec 2021 11:43:24: 48000000 INFO @ Fri, 10 Dec 2021 11:43:30: 49000000 INFO @ Fri, 10 Dec 2021 11:43:30: 49000000 INFO @ Fri, 10 Dec 2021 11:43:36: 50000000 INFO @ Fri, 10 Dec 2021 11:43:37: 50000000 INFO @ Fri, 10 Dec 2021 11:43:43: 51000000 INFO @ Fri, 10 Dec 2021 11:43:44: 51000000 INFO @ Fri, 10 Dec 2021 11:43:49: 52000000 INFO @ Fri, 10 Dec 2021 11:43:50: 52000000 INFO @ Fri, 10 Dec 2021 11:43:56: 53000000 INFO @ Fri, 10 Dec 2021 11:43:56: 53000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:44:02: 54000000 INFO @ Fri, 10 Dec 2021 11:44:03: 54000000 INFO @ Fri, 10 Dec 2021 11:44:08: 55000000 INFO @ Fri, 10 Dec 2021 11:44:09: 55000000 INFO @ Fri, 10 Dec 2021 11:44:15: 56000000 INFO @ Fri, 10 Dec 2021 11:44:15: 56000000 INFO @ Fri, 10 Dec 2021 11:44:21: 57000000 INFO @ Fri, 10 Dec 2021 11:44:22: 57000000 INFO @ Fri, 10 Dec 2021 11:44:22: #1 tag size is determined as 76 bps INFO @ Fri, 10 Dec 2021 11:44:22: #1 tag size = 76 INFO @ Fri, 10 Dec 2021 11:44:22: #1 total tags in treatment: 23116552 INFO @ Fri, 10 Dec 2021 11:44:22: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:44:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:44:23: #1 tags after filtering in treatment: 18646867 INFO @ Fri, 10 Dec 2021 11:44:23: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 10 Dec 2021 11:44:23: #1 finished! INFO @ Fri, 10 Dec 2021 11:44:23: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:44:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:44:23: #1 tag size is determined as 76 bps INFO @ Fri, 10 Dec 2021 11:44:23: #1 tag size = 76 INFO @ Fri, 10 Dec 2021 11:44:23: #1 total tags in treatment: 23116552 INFO @ Fri, 10 Dec 2021 11:44:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:44:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:44:23: #1 tags after filtering in treatment: 18646867 INFO @ Fri, 10 Dec 2021 11:44:23: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 10 Dec 2021 11:44:23: #1 finished! INFO @ Fri, 10 Dec 2021 11:44:23: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:44:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:44:24: #2 number of paired peaks: 56 WARNING @ Fri, 10 Dec 2021 11:44:24: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:44:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:44:24: #2 number of paired peaks: 56 WARNING @ Fri, 10 Dec 2021 11:44:24: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:44:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712929/SRX4712929.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling