Job ID = 14167083 SRX = SRX4712925 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 24987222 spots for SRR7874055/SRR7874055.sra Written 24987222 spots for SRR7874055/SRR7874055.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167577 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:39:26 24987222 reads; of these: 24987222 (100.00%) were paired; of these: 5570413 (22.29%) aligned concordantly 0 times 14320328 (57.31%) aligned concordantly exactly 1 time 5096481 (20.40%) aligned concordantly >1 times ---- 5570413 pairs aligned concordantly 0 times; of these: 2410382 (43.27%) aligned discordantly 1 time ---- 3160031 pairs aligned 0 times concordantly or discordantly; of these: 6320062 mates make up the pairs; of these: 4204130 (66.52%) aligned 0 times 803493 (12.71%) aligned exactly 1 time 1312439 (20.77%) aligned >1 times 91.59% overall alignment rate Time searching: 00:39:27 Overall time: 00:39:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1091402 / 21606332 = 0.0505 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:33:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:33:01: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:33:01: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:33:05: 1000000 INFO @ Fri, 10 Dec 2021 10:33:10: 2000000 INFO @ Fri, 10 Dec 2021 10:33:14: 3000000 INFO @ Fri, 10 Dec 2021 10:33:19: 4000000 INFO @ Fri, 10 Dec 2021 10:33:23: 5000000 INFO @ Fri, 10 Dec 2021 10:33:28: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:33:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:33:31: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:33:31: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:33:32: 7000000 INFO @ Fri, 10 Dec 2021 10:33:35: 1000000 INFO @ Fri, 10 Dec 2021 10:33:37: 8000000 INFO @ Fri, 10 Dec 2021 10:33:40: 2000000 INFO @ Fri, 10 Dec 2021 10:33:42: 9000000 INFO @ Fri, 10 Dec 2021 10:33:45: 3000000 INFO @ Fri, 10 Dec 2021 10:33:47: 10000000 INFO @ Fri, 10 Dec 2021 10:33:50: 4000000 INFO @ Fri, 10 Dec 2021 10:33:52: 11000000 INFO @ Fri, 10 Dec 2021 10:33:55: 5000000 INFO @ Fri, 10 Dec 2021 10:33:56: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:34:00: 6000000 INFO @ Fri, 10 Dec 2021 10:34:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:34:01: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:34:01: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:34:01: 13000000 INFO @ Fri, 10 Dec 2021 10:34:05: 7000000 INFO @ Fri, 10 Dec 2021 10:34:05: 1000000 INFO @ Fri, 10 Dec 2021 10:34:06: 14000000 INFO @ Fri, 10 Dec 2021 10:34:10: 8000000 INFO @ Fri, 10 Dec 2021 10:34:10: 2000000 INFO @ Fri, 10 Dec 2021 10:34:11: 15000000 INFO @ Fri, 10 Dec 2021 10:34:15: 9000000 INFO @ Fri, 10 Dec 2021 10:34:15: 3000000 INFO @ Fri, 10 Dec 2021 10:34:16: 16000000 INFO @ Fri, 10 Dec 2021 10:34:20: 10000000 INFO @ Fri, 10 Dec 2021 10:34:20: 4000000 INFO @ Fri, 10 Dec 2021 10:34:21: 17000000 INFO @ Fri, 10 Dec 2021 10:34:25: 11000000 INFO @ Fri, 10 Dec 2021 10:34:25: 5000000 INFO @ Fri, 10 Dec 2021 10:34:26: 18000000 INFO @ Fri, 10 Dec 2021 10:34:30: 12000000 INFO @ Fri, 10 Dec 2021 10:34:30: 6000000 INFO @ Fri, 10 Dec 2021 10:34:31: 19000000 INFO @ Fri, 10 Dec 2021 10:34:35: 7000000 INFO @ Fri, 10 Dec 2021 10:34:35: 13000000 INFO @ Fri, 10 Dec 2021 10:34:36: 20000000 INFO @ Fri, 10 Dec 2021 10:34:40: 8000000 INFO @ Fri, 10 Dec 2021 10:34:40: 14000000 INFO @ Fri, 10 Dec 2021 10:34:41: 21000000 INFO @ Fri, 10 Dec 2021 10:34:44: 9000000 INFO @ Fri, 10 Dec 2021 10:34:45: 15000000 INFO @ Fri, 10 Dec 2021 10:34:46: 22000000 INFO @ Fri, 10 Dec 2021 10:34:49: 10000000 INFO @ Fri, 10 Dec 2021 10:34:50: 16000000 INFO @ Fri, 10 Dec 2021 10:34:51: 23000000 INFO @ Fri, 10 Dec 2021 10:34:54: 11000000 INFO @ Fri, 10 Dec 2021 10:34:55: 17000000 INFO @ Fri, 10 Dec 2021 10:34:56: 24000000 INFO @ Fri, 10 Dec 2021 10:34:59: 12000000 INFO @ Fri, 10 Dec 2021 10:35:00: 18000000 INFO @ Fri, 10 Dec 2021 10:35:01: 25000000 INFO @ Fri, 10 Dec 2021 10:35:04: 13000000 INFO @ Fri, 10 Dec 2021 10:35:05: 19000000 INFO @ Fri, 10 Dec 2021 10:35:06: 26000000 INFO @ Fri, 10 Dec 2021 10:35:09: 14000000 INFO @ Fri, 10 Dec 2021 10:35:10: 20000000 INFO @ Fri, 10 Dec 2021 10:35:11: 27000000 INFO @ Fri, 10 Dec 2021 10:35:14: 15000000 INFO @ Fri, 10 Dec 2021 10:35:15: 21000000 INFO @ Fri, 10 Dec 2021 10:35:16: 28000000 INFO @ Fri, 10 Dec 2021 10:35:18: 16000000 INFO @ Fri, 10 Dec 2021 10:35:20: 22000000 INFO @ Fri, 10 Dec 2021 10:35:21: 29000000 INFO @ Fri, 10 Dec 2021 10:35:23: 17000000 INFO @ Fri, 10 Dec 2021 10:35:25: 23000000 INFO @ Fri, 10 Dec 2021 10:35:26: 30000000 INFO @ Fri, 10 Dec 2021 10:35:28: 18000000 INFO @ Fri, 10 Dec 2021 10:35:30: 24000000 INFO @ Fri, 10 Dec 2021 10:35:31: 31000000 INFO @ Fri, 10 Dec 2021 10:35:33: 19000000 INFO @ Fri, 10 Dec 2021 10:35:35: 25000000 INFO @ Fri, 10 Dec 2021 10:35:36: 32000000 INFO @ Fri, 10 Dec 2021 10:35:38: 20000000 INFO @ Fri, 10 Dec 2021 10:35:40: 26000000 INFO @ Fri, 10 Dec 2021 10:35:42: 33000000 INFO @ Fri, 10 Dec 2021 10:35:43: 21000000 INFO @ Fri, 10 Dec 2021 10:35:45: 27000000 INFO @ Fri, 10 Dec 2021 10:35:47: 34000000 INFO @ Fri, 10 Dec 2021 10:35:48: 22000000 INFO @ Fri, 10 Dec 2021 10:35:50: 28000000 INFO @ Fri, 10 Dec 2021 10:35:52: 35000000 INFO @ Fri, 10 Dec 2021 10:35:53: 23000000 INFO @ Fri, 10 Dec 2021 10:35:55: 29000000 INFO @ Fri, 10 Dec 2021 10:35:57: 36000000 INFO @ Fri, 10 Dec 2021 10:35:57: 24000000 INFO @ Fri, 10 Dec 2021 10:36:00: 30000000 INFO @ Fri, 10 Dec 2021 10:36:02: 37000000 INFO @ Fri, 10 Dec 2021 10:36:02: 25000000 INFO @ Fri, 10 Dec 2021 10:36:05: 31000000 INFO @ Fri, 10 Dec 2021 10:36:07: 38000000 INFO @ Fri, 10 Dec 2021 10:36:07: 26000000 INFO @ Fri, 10 Dec 2021 10:36:10: 32000000 INFO @ Fri, 10 Dec 2021 10:36:12: 39000000 INFO @ Fri, 10 Dec 2021 10:36:12: 27000000 INFO @ Fri, 10 Dec 2021 10:36:15: 33000000 INFO @ Fri, 10 Dec 2021 10:36:17: 40000000 INFO @ Fri, 10 Dec 2021 10:36:17: 28000000 INFO @ Fri, 10 Dec 2021 10:36:20: 34000000 INFO @ Fri, 10 Dec 2021 10:36:22: 41000000 INFO @ Fri, 10 Dec 2021 10:36:22: 29000000 INFO @ Fri, 10 Dec 2021 10:36:25: 35000000 INFO @ Fri, 10 Dec 2021 10:36:27: 30000000 INFO @ Fri, 10 Dec 2021 10:36:27: 42000000 INFO @ Fri, 10 Dec 2021 10:36:30: 36000000 INFO @ Fri, 10 Dec 2021 10:36:32: 31000000 INFO @ Fri, 10 Dec 2021 10:36:32: 43000000 INFO @ Fri, 10 Dec 2021 10:36:35: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:36:35: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:36:35: #1 total tags in treatment: 18382414 INFO @ Fri, 10 Dec 2021 10:36:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:36:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:36:35: #1 tags after filtering in treatment: 14734371 INFO @ Fri, 10 Dec 2021 10:36:35: #1 Redundant rate of treatment: 0.20 INFO @ Fri, 10 Dec 2021 10:36:35: #1 finished! INFO @ Fri, 10 Dec 2021 10:36:35: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:36:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:36:36: 37000000 INFO @ Fri, 10 Dec 2021 10:36:36: #2 number of paired peaks: 94 WARNING @ Fri, 10 Dec 2021 10:36:36: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:36:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:36:36: 32000000 INFO @ Fri, 10 Dec 2021 10:36:40: 38000000 INFO @ Fri, 10 Dec 2021 10:36:41: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:36:45: 39000000 INFO @ Fri, 10 Dec 2021 10:36:46: 34000000 INFO @ Fri, 10 Dec 2021 10:36:50: 40000000 INFO @ Fri, 10 Dec 2021 10:36:51: 35000000 INFO @ Fri, 10 Dec 2021 10:36:55: 41000000 INFO @ Fri, 10 Dec 2021 10:36:56: 36000000 INFO @ Fri, 10 Dec 2021 10:37:00: 42000000 INFO @ Fri, 10 Dec 2021 10:37:01: 37000000 INFO @ Fri, 10 Dec 2021 10:37:04: 43000000 INFO @ Fri, 10 Dec 2021 10:37:06: 38000000 INFO @ Fri, 10 Dec 2021 10:37:07: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:37:07: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:37:07: #1 total tags in treatment: 18382414 INFO @ Fri, 10 Dec 2021 10:37:07: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:37:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:37:08: #1 tags after filtering in treatment: 14734371 INFO @ Fri, 10 Dec 2021 10:37:08: #1 Redundant rate of treatment: 0.20 INFO @ Fri, 10 Dec 2021 10:37:08: #1 finished! INFO @ Fri, 10 Dec 2021 10:37:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:37:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:37:09: #2 number of paired peaks: 94 WARNING @ Fri, 10 Dec 2021 10:37:09: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:37:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:37:11: 39000000 INFO @ Fri, 10 Dec 2021 10:37:15: 40000000 INFO @ Fri, 10 Dec 2021 10:37:20: 41000000 INFO @ Fri, 10 Dec 2021 10:37:24: 42000000 INFO @ Fri, 10 Dec 2021 10:37:29: 43000000 INFO @ Fri, 10 Dec 2021 10:37:32: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 10:37:32: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 10:37:32: #1 total tags in treatment: 18382414 INFO @ Fri, 10 Dec 2021 10:37:32: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:37:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:37:32: #1 tags after filtering in treatment: 14734371 INFO @ Fri, 10 Dec 2021 10:37:32: #1 Redundant rate of treatment: 0.20 INFO @ Fri, 10 Dec 2021 10:37:32: #1 finished! INFO @ Fri, 10 Dec 2021 10:37:32: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:37:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:37:33: #2 number of paired peaks: 94 WARNING @ Fri, 10 Dec 2021 10:37:33: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:37:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4712925/SRX4712925.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。