Job ID = 6498281 SRX = SRX467047 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:25:02 prefetch.2.10.7: 1) Downloading 'SRR1164471'... 2020-06-25T23:25:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:29:58 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:29:58 prefetch.2.10.7: 1) 'SRR1164471' was downloaded successfully Read 42670748 spots for SRR1164471/SRR1164471.sra Written 42670748 spots for SRR1164471/SRR1164471.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:36 42670748 reads; of these: 42670748 (100.00%) were unpaired; of these: 7487972 (17.55%) aligned 0 times 27298003 (63.97%) aligned exactly 1 time 7884773 (18.48%) aligned >1 times 82.45% overall alignment rate Time searching: 00:13:36 Overall time: 00:13:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11411181 / 35182776 = 0.3243 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:53:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:53:33: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:53:33: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:53:38: 1000000 INFO @ Fri, 26 Jun 2020 08:53:44: 2000000 INFO @ Fri, 26 Jun 2020 08:53:50: 3000000 INFO @ Fri, 26 Jun 2020 08:53:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:54:01: 5000000 INFO @ Fri, 26 Jun 2020 08:54:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:54:03: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:54:03: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:54:07: 6000000 INFO @ Fri, 26 Jun 2020 08:54:09: 1000000 INFO @ Fri, 26 Jun 2020 08:54:13: 7000000 INFO @ Fri, 26 Jun 2020 08:54:15: 2000000 INFO @ Fri, 26 Jun 2020 08:54:19: 8000000 INFO @ Fri, 26 Jun 2020 08:54:21: 3000000 INFO @ Fri, 26 Jun 2020 08:54:25: 9000000 INFO @ Fri, 26 Jun 2020 08:54:27: 4000000 INFO @ Fri, 26 Jun 2020 08:54:31: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:54:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:54:33: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:54:33: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:54:33: 5000000 INFO @ Fri, 26 Jun 2020 08:54:37: 11000000 INFO @ Fri, 26 Jun 2020 08:54:39: 1000000 INFO @ Fri, 26 Jun 2020 08:54:39: 6000000 INFO @ Fri, 26 Jun 2020 08:54:43: 12000000 INFO @ Fri, 26 Jun 2020 08:54:45: 2000000 INFO @ Fri, 26 Jun 2020 08:54:45: 7000000 INFO @ Fri, 26 Jun 2020 08:54:49: 13000000 INFO @ Fri, 26 Jun 2020 08:54:51: 3000000 INFO @ Fri, 26 Jun 2020 08:54:51: 8000000 INFO @ Fri, 26 Jun 2020 08:54:55: 14000000 INFO @ Fri, 26 Jun 2020 08:54:57: 4000000 INFO @ Fri, 26 Jun 2020 08:54:57: 9000000 INFO @ Fri, 26 Jun 2020 08:55:01: 15000000 INFO @ Fri, 26 Jun 2020 08:55:03: 5000000 INFO @ Fri, 26 Jun 2020 08:55:03: 10000000 INFO @ Fri, 26 Jun 2020 08:55:07: 16000000 INFO @ Fri, 26 Jun 2020 08:55:09: 6000000 INFO @ Fri, 26 Jun 2020 08:55:09: 11000000 INFO @ Fri, 26 Jun 2020 08:55:13: 17000000 INFO @ Fri, 26 Jun 2020 08:55:15: 7000000 INFO @ Fri, 26 Jun 2020 08:55:15: 12000000 INFO @ Fri, 26 Jun 2020 08:55:19: 18000000 INFO @ Fri, 26 Jun 2020 08:55:21: 8000000 INFO @ Fri, 26 Jun 2020 08:55:21: 13000000 INFO @ Fri, 26 Jun 2020 08:55:25: 19000000 INFO @ Fri, 26 Jun 2020 08:55:27: 9000000 INFO @ Fri, 26 Jun 2020 08:55:27: 14000000 INFO @ Fri, 26 Jun 2020 08:55:31: 20000000 INFO @ Fri, 26 Jun 2020 08:55:33: 10000000 INFO @ Fri, 26 Jun 2020 08:55:33: 15000000 INFO @ Fri, 26 Jun 2020 08:55:37: 21000000 INFO @ Fri, 26 Jun 2020 08:55:39: 16000000 INFO @ Fri, 26 Jun 2020 08:55:39: 11000000 INFO @ Fri, 26 Jun 2020 08:55:43: 22000000 INFO @ Fri, 26 Jun 2020 08:55:45: 17000000 INFO @ Fri, 26 Jun 2020 08:55:45: 12000000 INFO @ Fri, 26 Jun 2020 08:55:49: 23000000 INFO @ Fri, 26 Jun 2020 08:55:51: 18000000 INFO @ Fri, 26 Jun 2020 08:55:52: 13000000 INFO @ Fri, 26 Jun 2020 08:55:54: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:55:54: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:55:54: #1 total tags in treatment: 23771595 INFO @ Fri, 26 Jun 2020 08:55:54: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:55:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:55:54: #1 tags after filtering in treatment: 23771595 INFO @ Fri, 26 Jun 2020 08:55:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:55:54: #1 finished! INFO @ Fri, 26 Jun 2020 08:55:54: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:55:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:55:55: #2 number of paired peaks: 38 WARNING @ Fri, 26 Jun 2020 08:55:55: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:55:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:55:57: 19000000 INFO @ Fri, 26 Jun 2020 08:55:58: 14000000 INFO @ Fri, 26 Jun 2020 08:56:04: 20000000 INFO @ Fri, 26 Jun 2020 08:56:04: 15000000 INFO @ Fri, 26 Jun 2020 08:56:10: 21000000 INFO @ Fri, 26 Jun 2020 08:56:10: 16000000 INFO @ Fri, 26 Jun 2020 08:56:16: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:56:16: 17000000 INFO @ Fri, 26 Jun 2020 08:56:22: 23000000 INFO @ Fri, 26 Jun 2020 08:56:22: 18000000 INFO @ Fri, 26 Jun 2020 08:56:27: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:56:27: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:56:27: #1 total tags in treatment: 23771595 INFO @ Fri, 26 Jun 2020 08:56:27: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:56:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:56:27: #1 tags after filtering in treatment: 23771595 INFO @ Fri, 26 Jun 2020 08:56:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:56:27: #1 finished! INFO @ Fri, 26 Jun 2020 08:56:27: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:56:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:56:29: 19000000 INFO @ Fri, 26 Jun 2020 08:56:29: #2 number of paired peaks: 38 WARNING @ Fri, 26 Jun 2020 08:56:29: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:56:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:56:34: 20000000 INFO @ Fri, 26 Jun 2020 08:56:40: 21000000 INFO @ Fri, 26 Jun 2020 08:56:46: 22000000 INFO @ Fri, 26 Jun 2020 08:56:52: 23000000 INFO @ Fri, 26 Jun 2020 08:56:57: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:56:57: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:56:57: #1 total tags in treatment: 23771595 INFO @ Fri, 26 Jun 2020 08:56:57: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:56:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:56:57: #1 tags after filtering in treatment: 23771595 INFO @ Fri, 26 Jun 2020 08:56:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:56:57: #1 finished! INFO @ Fri, 26 Jun 2020 08:56:57: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:56:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:56:59: #2 number of paired peaks: 38 WARNING @ Fri, 26 Jun 2020 08:56:59: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:56:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467047/SRX467047.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。