Job ID = 1298689 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 48,787,082 reads read : 48,787,082 reads written : 48,787,082 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:13 48787082 reads; of these: 48787082 (100.00%) were unpaired; of these: 2537020 (5.20%) aligned 0 times 37963452 (77.81%) aligned exactly 1 time 8286610 (16.99%) aligned >1 times 94.80% overall alignment rate Time searching: 00:17:13 Overall time: 00:17:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13253159 / 46250062 = 0.2866 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 18:18:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:18:40: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:18:40: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:18:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:18:40: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:18:40: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:18:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:18:40: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:18:40: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:18:51: 1000000 INFO @ Mon, 03 Jun 2019 18:18:51: 1000000 INFO @ Mon, 03 Jun 2019 18:18:52: 1000000 INFO @ Mon, 03 Jun 2019 18:19:01: 2000000 INFO @ Mon, 03 Jun 2019 18:19:01: 2000000 INFO @ Mon, 03 Jun 2019 18:19:04: 2000000 INFO @ Mon, 03 Jun 2019 18:19:12: 3000000 INFO @ Mon, 03 Jun 2019 18:19:12: 3000000 INFO @ Mon, 03 Jun 2019 18:19:15: 3000000 INFO @ Mon, 03 Jun 2019 18:19:22: 4000000 INFO @ Mon, 03 Jun 2019 18:19:22: 4000000 INFO @ Mon, 03 Jun 2019 18:19:27: 4000000 INFO @ Mon, 03 Jun 2019 18:19:33: 5000000 INFO @ Mon, 03 Jun 2019 18:19:33: 5000000 INFO @ Mon, 03 Jun 2019 18:19:38: 5000000 INFO @ Mon, 03 Jun 2019 18:19:44: 6000000 INFO @ Mon, 03 Jun 2019 18:19:44: 6000000 INFO @ Mon, 03 Jun 2019 18:19:50: 6000000 INFO @ Mon, 03 Jun 2019 18:19:54: 7000000 INFO @ Mon, 03 Jun 2019 18:19:55: 7000000 INFO @ Mon, 03 Jun 2019 18:20:02: 7000000 INFO @ Mon, 03 Jun 2019 18:20:05: 8000000 INFO @ Mon, 03 Jun 2019 18:20:05: 8000000 INFO @ Mon, 03 Jun 2019 18:20:11: 8000000 INFO @ Mon, 03 Jun 2019 18:20:14: 9000000 INFO @ Mon, 03 Jun 2019 18:20:15: 9000000 INFO @ Mon, 03 Jun 2019 18:20:20: 9000000 INFO @ Mon, 03 Jun 2019 18:20:25: 10000000 INFO @ Mon, 03 Jun 2019 18:20:26: 10000000 INFO @ Mon, 03 Jun 2019 18:20:29: 10000000 INFO @ Mon, 03 Jun 2019 18:20:35: 11000000 INFO @ Mon, 03 Jun 2019 18:20:35: 11000000 INFO @ Mon, 03 Jun 2019 18:20:37: 11000000 INFO @ Mon, 03 Jun 2019 18:20:45: 12000000 INFO @ Mon, 03 Jun 2019 18:20:45: 12000000 INFO @ Mon, 03 Jun 2019 18:20:45: 12000000 INFO @ Mon, 03 Jun 2019 18:20:54: 13000000 INFO @ Mon, 03 Jun 2019 18:20:54: 13000000 INFO @ Mon, 03 Jun 2019 18:20:55: 13000000 INFO @ Mon, 03 Jun 2019 18:21:02: 14000000 INFO @ Mon, 03 Jun 2019 18:21:04: 14000000 INFO @ Mon, 03 Jun 2019 18:21:05: 14000000 INFO @ Mon, 03 Jun 2019 18:21:10: 15000000 INFO @ Mon, 03 Jun 2019 18:21:14: 15000000 INFO @ Mon, 03 Jun 2019 18:21:15: 15000000 INFO @ Mon, 03 Jun 2019 18:21:18: 16000000 INFO @ Mon, 03 Jun 2019 18:21:24: 16000000 INFO @ Mon, 03 Jun 2019 18:21:25: 16000000 INFO @ Mon, 03 Jun 2019 18:21:26: 17000000 INFO @ Mon, 03 Jun 2019 18:21:34: 17000000 INFO @ Mon, 03 Jun 2019 18:21:35: 17000000 INFO @ Mon, 03 Jun 2019 18:21:35: 18000000 INFO @ Mon, 03 Jun 2019 18:21:43: 19000000 INFO @ Mon, 03 Jun 2019 18:21:43: 18000000 INFO @ Mon, 03 Jun 2019 18:21:44: 18000000 INFO @ Mon, 03 Jun 2019 18:21:51: 20000000 INFO @ Mon, 03 Jun 2019 18:21:53: 19000000 INFO @ Mon, 03 Jun 2019 18:21:54: 19000000 INFO @ Mon, 03 Jun 2019 18:21:59: 21000000 INFO @ Mon, 03 Jun 2019 18:22:03: 20000000 INFO @ Mon, 03 Jun 2019 18:22:04: 20000000 INFO @ Mon, 03 Jun 2019 18:22:07: 22000000 INFO @ Mon, 03 Jun 2019 18:22:13: 21000000 INFO @ Mon, 03 Jun 2019 18:22:14: 21000000 INFO @ Mon, 03 Jun 2019 18:22:16: 23000000 INFO @ Mon, 03 Jun 2019 18:22:23: 22000000 INFO @ Mon, 03 Jun 2019 18:22:23: 22000000 INFO @ Mon, 03 Jun 2019 18:22:24: 24000000 INFO @ Mon, 03 Jun 2019 18:22:33: 23000000 INFO @ Mon, 03 Jun 2019 18:22:34: 25000000 INFO @ Mon, 03 Jun 2019 18:22:34: 23000000 INFO @ Mon, 03 Jun 2019 18:22:43: 26000000 INFO @ Mon, 03 Jun 2019 18:22:44: 24000000 INFO @ Mon, 03 Jun 2019 18:22:44: 24000000 INFO @ Mon, 03 Jun 2019 18:22:52: 27000000 INFO @ Mon, 03 Jun 2019 18:22:54: 25000000 INFO @ Mon, 03 Jun 2019 18:22:54: 25000000 INFO @ Mon, 03 Jun 2019 18:23:01: 28000000 INFO @ Mon, 03 Jun 2019 18:23:04: 26000000 INFO @ Mon, 03 Jun 2019 18:23:05: 26000000 INFO @ Mon, 03 Jun 2019 18:23:10: 29000000 INFO @ Mon, 03 Jun 2019 18:23:15: 27000000 INFO @ Mon, 03 Jun 2019 18:23:15: 27000000 INFO @ Mon, 03 Jun 2019 18:23:19: 30000000 INFO @ Mon, 03 Jun 2019 18:23:25: 28000000 INFO @ Mon, 03 Jun 2019 18:23:26: 28000000 INFO @ Mon, 03 Jun 2019 18:23:28: 31000000 INFO @ Mon, 03 Jun 2019 18:23:36: 29000000 INFO @ Mon, 03 Jun 2019 18:23:36: 29000000 INFO @ Mon, 03 Jun 2019 18:23:38: 32000000 INFO @ Mon, 03 Jun 2019 18:23:46: 30000000 INFO @ Mon, 03 Jun 2019 18:23:47: 30000000 INFO @ Mon, 03 Jun 2019 18:23:48: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:23:48: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:23:48: #1 total tags in treatment: 32996903 INFO @ Mon, 03 Jun 2019 18:23:48: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:23:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:23:49: #1 tags after filtering in treatment: 32996903 INFO @ Mon, 03 Jun 2019 18:23:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:23:49: #1 finished! INFO @ Mon, 03 Jun 2019 18:23:49: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:23:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:23:52: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:23:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:23:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:23:56: 31000000 INFO @ Mon, 03 Jun 2019 18:23:57: 31000000 INFO @ Mon, 03 Jun 2019 18:24:07: 32000000 INFO @ Mon, 03 Jun 2019 18:24:08: 32000000 INFO @ Mon, 03 Jun 2019 18:24:17: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:24:17: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:24:17: #1 total tags in treatment: 32996903 INFO @ Mon, 03 Jun 2019 18:24:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:24:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:24:18: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:24:18: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:24:18: #1 total tags in treatment: 32996903 INFO @ Mon, 03 Jun 2019 18:24:18: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:24:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:24:18: #1 tags after filtering in treatment: 32996903 INFO @ Mon, 03 Jun 2019 18:24:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:24:18: #1 finished! INFO @ Mon, 03 Jun 2019 18:24:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:24:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:24:18: #1 tags after filtering in treatment: 32996903 INFO @ Mon, 03 Jun 2019 18:24:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:24:18: #1 finished! INFO @ Mon, 03 Jun 2019 18:24:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:24:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:24:21: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:24:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:24:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:24:21: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:24:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:24:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467045/SRX467045.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。