Job ID = 1298632 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 48,787,082 reads read : 48,787,082 reads written : 48,787,082 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:38 48787082 reads; of these: 48787082 (100.00%) were unpaired; of these: 2537031 (5.20%) aligned 0 times 37963302 (77.81%) aligned exactly 1 time 8286749 (16.99%) aligned >1 times 94.80% overall alignment rate Time searching: 00:17:38 Overall time: 00:17:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13253688 / 46250051 = 0.2866 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 18:18:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:18:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:18:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:18:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:18:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:18:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:18:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 18:18:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 18:18:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 18:19:08: 1000000 INFO @ Mon, 03 Jun 2019 18:19:09: 1000000 INFO @ Mon, 03 Jun 2019 18:19:09: 1000000 INFO @ Mon, 03 Jun 2019 18:19:16: 2000000 INFO @ Mon, 03 Jun 2019 18:19:19: 2000000 INFO @ Mon, 03 Jun 2019 18:19:19: 2000000 INFO @ Mon, 03 Jun 2019 18:19:25: 3000000 INFO @ Mon, 03 Jun 2019 18:19:28: 3000000 INFO @ Mon, 03 Jun 2019 18:19:29: 3000000 INFO @ Mon, 03 Jun 2019 18:19:33: 4000000 INFO @ Mon, 03 Jun 2019 18:19:38: 4000000 INFO @ Mon, 03 Jun 2019 18:19:39: 4000000 INFO @ Mon, 03 Jun 2019 18:19:42: 5000000 INFO @ Mon, 03 Jun 2019 18:19:47: 5000000 INFO @ Mon, 03 Jun 2019 18:19:48: 5000000 INFO @ Mon, 03 Jun 2019 18:19:50: 6000000 INFO @ Mon, 03 Jun 2019 18:19:57: 6000000 INFO @ Mon, 03 Jun 2019 18:19:58: 6000000 INFO @ Mon, 03 Jun 2019 18:19:59: 7000000 INFO @ Mon, 03 Jun 2019 18:20:07: 7000000 INFO @ Mon, 03 Jun 2019 18:20:08: 8000000 INFO @ Mon, 03 Jun 2019 18:20:08: 7000000 INFO @ Mon, 03 Jun 2019 18:20:16: 8000000 INFO @ Mon, 03 Jun 2019 18:20:17: 9000000 INFO @ Mon, 03 Jun 2019 18:20:19: 8000000 INFO @ Mon, 03 Jun 2019 18:20:25: 10000000 INFO @ Mon, 03 Jun 2019 18:20:26: 9000000 INFO @ Mon, 03 Jun 2019 18:20:28: 9000000 INFO @ Mon, 03 Jun 2019 18:20:34: 11000000 INFO @ Mon, 03 Jun 2019 18:20:35: 10000000 INFO @ Mon, 03 Jun 2019 18:20:38: 10000000 INFO @ Mon, 03 Jun 2019 18:20:42: 12000000 INFO @ Mon, 03 Jun 2019 18:20:45: 11000000 INFO @ Mon, 03 Jun 2019 18:20:48: 11000000 INFO @ Mon, 03 Jun 2019 18:20:51: 13000000 INFO @ Mon, 03 Jun 2019 18:20:54: 12000000 INFO @ Mon, 03 Jun 2019 18:20:57: 12000000 INFO @ Mon, 03 Jun 2019 18:20:59: 14000000 INFO @ Mon, 03 Jun 2019 18:21:03: 13000000 INFO @ Mon, 03 Jun 2019 18:21:07: 13000000 INFO @ Mon, 03 Jun 2019 18:21:07: 15000000 INFO @ Mon, 03 Jun 2019 18:21:12: 14000000 INFO @ Mon, 03 Jun 2019 18:21:15: 16000000 INFO @ Mon, 03 Jun 2019 18:21:16: 14000000 INFO @ Mon, 03 Jun 2019 18:21:21: 15000000 INFO @ Mon, 03 Jun 2019 18:21:23: 17000000 INFO @ Mon, 03 Jun 2019 18:21:25: 15000000 INFO @ Mon, 03 Jun 2019 18:21:31: 18000000 INFO @ Mon, 03 Jun 2019 18:21:31: 16000000 INFO @ Mon, 03 Jun 2019 18:21:35: 16000000 INFO @ Mon, 03 Jun 2019 18:21:38: 19000000 INFO @ Mon, 03 Jun 2019 18:21:40: 17000000 INFO @ Mon, 03 Jun 2019 18:21:45: 17000000 INFO @ Mon, 03 Jun 2019 18:21:46: 20000000 INFO @ Mon, 03 Jun 2019 18:21:49: 18000000 INFO @ Mon, 03 Jun 2019 18:21:54: 21000000 INFO @ Mon, 03 Jun 2019 18:21:55: 18000000 INFO @ Mon, 03 Jun 2019 18:21:58: 19000000 INFO @ Mon, 03 Jun 2019 18:22:02: 22000000 INFO @ Mon, 03 Jun 2019 18:22:05: 19000000 INFO @ Mon, 03 Jun 2019 18:22:07: 20000000 INFO @ Mon, 03 Jun 2019 18:22:10: 23000000 INFO @ Mon, 03 Jun 2019 18:22:14: 20000000 INFO @ Mon, 03 Jun 2019 18:22:16: 21000000 INFO @ Mon, 03 Jun 2019 18:22:18: 24000000 INFO @ Mon, 03 Jun 2019 18:22:23: 21000000 INFO @ Mon, 03 Jun 2019 18:22:25: 22000000 INFO @ Mon, 03 Jun 2019 18:22:26: 25000000 INFO @ Mon, 03 Jun 2019 18:22:32: 22000000 INFO @ Mon, 03 Jun 2019 18:22:34: 26000000 INFO @ Mon, 03 Jun 2019 18:22:34: 23000000 INFO @ Mon, 03 Jun 2019 18:22:42: 23000000 INFO @ Mon, 03 Jun 2019 18:22:42: 27000000 INFO @ Mon, 03 Jun 2019 18:22:43: 24000000 INFO @ Mon, 03 Jun 2019 18:22:50: 28000000 INFO @ Mon, 03 Jun 2019 18:22:51: 24000000 INFO @ Mon, 03 Jun 2019 18:22:52: 25000000 INFO @ Mon, 03 Jun 2019 18:22:58: 29000000 INFO @ Mon, 03 Jun 2019 18:23:00: 25000000 INFO @ Mon, 03 Jun 2019 18:23:01: 26000000 INFO @ Mon, 03 Jun 2019 18:23:06: 30000000 INFO @ Mon, 03 Jun 2019 18:23:10: 26000000 INFO @ Mon, 03 Jun 2019 18:23:11: 27000000 INFO @ Mon, 03 Jun 2019 18:23:13: 31000000 INFO @ Mon, 03 Jun 2019 18:23:19: 27000000 INFO @ Mon, 03 Jun 2019 18:23:20: 28000000 INFO @ Mon, 03 Jun 2019 18:23:21: 32000000 INFO @ Mon, 03 Jun 2019 18:23:29: 28000000 INFO @ Mon, 03 Jun 2019 18:23:29: 29000000 INFO @ Mon, 03 Jun 2019 18:23:30: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:23:30: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:23:30: #1 total tags in treatment: 32996363 INFO @ Mon, 03 Jun 2019 18:23:30: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:23:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:23:30: #1 tags after filtering in treatment: 32996363 INFO @ Mon, 03 Jun 2019 18:23:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:23:30: #1 finished! INFO @ Mon, 03 Jun 2019 18:23:30: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:23:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:23:33: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:23:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:23:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:23:38: 30000000 INFO @ Mon, 03 Jun 2019 18:23:38: 29000000 INFO @ Mon, 03 Jun 2019 18:23:47: 31000000 INFO @ Mon, 03 Jun 2019 18:23:47: 30000000 INFO @ Mon, 03 Jun 2019 18:23:56: 32000000 INFO @ Mon, 03 Jun 2019 18:23:57: 31000000 INFO @ Mon, 03 Jun 2019 18:24:05: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:24:05: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:24:05: #1 total tags in treatment: 32996363 INFO @ Mon, 03 Jun 2019 18:24:05: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:24:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:24:06: #1 tags after filtering in treatment: 32996363 INFO @ Mon, 03 Jun 2019 18:24:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:24:06: #1 finished! INFO @ Mon, 03 Jun 2019 18:24:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:24:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:24:06: 32000000 INFO @ Mon, 03 Jun 2019 18:24:08: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:24:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:24:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 18:24:15: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 18:24:15: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 18:24:15: #1 total tags in treatment: 32996363 INFO @ Mon, 03 Jun 2019 18:24:15: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 18:24:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 18:24:16: #1 tags after filtering in treatment: 32996363 INFO @ Mon, 03 Jun 2019 18:24:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 18:24:16: #1 finished! INFO @ Mon, 03 Jun 2019 18:24:16: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 18:24:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 18:24:18: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 18:24:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 18:24:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467044/SRX467044.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。