Job ID = 6528119 SRX = SRX467042 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:32:52 prefetch.2.10.7: 1) Downloading 'SRR1164466'... 2020-06-29T14:32:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:38:12 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:38:12 prefetch.2.10.7: 1) 'SRR1164466' was downloaded successfully Read 28609687 spots for SRR1164466/SRR1164466.sra Written 28609687 spots for SRR1164466/SRR1164466.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:14 28609687 reads; of these: 28609687 (100.00%) were unpaired; of these: 2975575 (10.40%) aligned 0 times 17513702 (61.22%) aligned exactly 1 time 8120410 (28.38%) aligned >1 times 89.60% overall alignment rate Time searching: 00:10:14 Overall time: 00:10:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8271859 / 25634112 = 0.3227 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:02:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:02:58: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:02:58: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:04: 1000000 INFO @ Tue, 30 Jun 2020 00:03:10: 2000000 INFO @ Tue, 30 Jun 2020 00:03:16: 3000000 INFO @ Tue, 30 Jun 2020 00:03:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:28: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:28: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:28: 5000000 INFO @ Tue, 30 Jun 2020 00:03:34: 1000000 INFO @ Tue, 30 Jun 2020 00:03:35: 6000000 INFO @ Tue, 30 Jun 2020 00:03:40: 2000000 INFO @ Tue, 30 Jun 2020 00:03:41: 7000000 INFO @ Tue, 30 Jun 2020 00:03:45: 3000000 INFO @ Tue, 30 Jun 2020 00:03:47: 8000000 INFO @ Tue, 30 Jun 2020 00:03:51: 4000000 INFO @ Tue, 30 Jun 2020 00:03:54: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:57: 5000000 INFO @ Tue, 30 Jun 2020 00:03:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:57: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:57: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:04:00: 10000000 INFO @ Tue, 30 Jun 2020 00:04:03: 6000000 INFO @ Tue, 30 Jun 2020 00:04:04: 1000000 INFO @ Tue, 30 Jun 2020 00:04:06: 11000000 INFO @ Tue, 30 Jun 2020 00:04:09: 7000000 INFO @ Tue, 30 Jun 2020 00:04:10: 2000000 INFO @ Tue, 30 Jun 2020 00:04:12: 12000000 INFO @ Tue, 30 Jun 2020 00:04:15: 8000000 INFO @ Tue, 30 Jun 2020 00:04:17: 3000000 INFO @ Tue, 30 Jun 2020 00:04:18: 13000000 INFO @ Tue, 30 Jun 2020 00:04:21: 9000000 INFO @ Tue, 30 Jun 2020 00:04:23: 4000000 INFO @ Tue, 30 Jun 2020 00:04:25: 14000000 INFO @ Tue, 30 Jun 2020 00:04:26: 10000000 INFO @ Tue, 30 Jun 2020 00:04:29: 5000000 INFO @ Tue, 30 Jun 2020 00:04:31: 15000000 INFO @ Tue, 30 Jun 2020 00:04:32: 11000000 INFO @ Tue, 30 Jun 2020 00:04:36: 6000000 INFO @ Tue, 30 Jun 2020 00:04:37: 16000000 INFO @ Tue, 30 Jun 2020 00:04:38: 12000000 INFO @ Tue, 30 Jun 2020 00:04:42: 7000000 INFO @ Tue, 30 Jun 2020 00:04:43: 17000000 INFO @ Tue, 30 Jun 2020 00:04:43: 13000000 INFO @ Tue, 30 Jun 2020 00:04:45: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:04:45: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:04:45: #1 total tags in treatment: 17362253 INFO @ Tue, 30 Jun 2020 00:04:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:04:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:04:46: #1 tags after filtering in treatment: 17362253 INFO @ Tue, 30 Jun 2020 00:04:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:04:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:04:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:04:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:04:47: #2 number of paired peaks: 71 WARNING @ Tue, 30 Jun 2020 00:04:47: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:04:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:04:48: 8000000 INFO @ Tue, 30 Jun 2020 00:04:49: 14000000 INFO @ Tue, 30 Jun 2020 00:04:55: 9000000 INFO @ Tue, 30 Jun 2020 00:04:55: 15000000 INFO @ Tue, 30 Jun 2020 00:05:00: 16000000 INFO @ Tue, 30 Jun 2020 00:05:01: 10000000 INFO @ Tue, 30 Jun 2020 00:05:06: 17000000 INFO @ Tue, 30 Jun 2020 00:05:07: 11000000 INFO @ Tue, 30 Jun 2020 00:05:08: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:05:08: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:05:08: #1 total tags in treatment: 17362253 INFO @ Tue, 30 Jun 2020 00:05:08: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:08: #1 tags after filtering in treatment: 17362253 INFO @ Tue, 30 Jun 2020 00:05:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:08: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:08: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:10: #2 number of paired peaks: 71 WARNING @ Tue, 30 Jun 2020 00:05:10: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:05:13: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:05:19: 13000000 INFO @ Tue, 30 Jun 2020 00:05:25: 14000000 INFO @ Tue, 30 Jun 2020 00:05:31: 15000000 INFO @ Tue, 30 Jun 2020 00:05:37: 16000000 INFO @ Tue, 30 Jun 2020 00:05:43: 17000000 INFO @ Tue, 30 Jun 2020 00:05:46: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:05:46: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:05:46: #1 total tags in treatment: 17362253 INFO @ Tue, 30 Jun 2020 00:05:46: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:46: #1 tags after filtering in treatment: 17362253 INFO @ Tue, 30 Jun 2020 00:05:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:47: #2 number of paired peaks: 71 WARNING @ Tue, 30 Jun 2020 00:05:47: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467042/SRX467042.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。