Job ID = 4303108 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 30,541,895 reads read : 30,541,895 reads written : 30,541,895 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164446.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:52 30541895 reads; of these: 30541895 (100.00%) were unpaired; of these: 8614734 (28.21%) aligned 0 times 18360625 (60.12%) aligned exactly 1 time 3566536 (11.68%) aligned >1 times 71.79% overall alignment rate Time searching: 00:06:52 Overall time: 00:06:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4955992 / 21927161 = 0.2260 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 00:57:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:57:06: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:57:06: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:57:12: 1000000 INFO @ Thu, 12 Dec 2019 00:57:18: 2000000 INFO @ Thu, 12 Dec 2019 00:57:24: 3000000 INFO @ Thu, 12 Dec 2019 00:57:31: 4000000 INFO @ Thu, 12 Dec 2019 00:57:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:57:36: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:57:36: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:57:37: 5000000 INFO @ Thu, 12 Dec 2019 00:57:43: 1000000 INFO @ Thu, 12 Dec 2019 00:57:44: 6000000 INFO @ Thu, 12 Dec 2019 00:57:50: 2000000 INFO @ Thu, 12 Dec 2019 00:57:51: 7000000 INFO @ Thu, 12 Dec 2019 00:57:57: 3000000 INFO @ Thu, 12 Dec 2019 00:57:58: 8000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 00:58:04: 4000000 INFO @ Thu, 12 Dec 2019 00:58:05: 9000000 INFO @ Thu, 12 Dec 2019 00:58:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:58:06: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:58:06: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:58:11: 5000000 INFO @ Thu, 12 Dec 2019 00:58:12: 10000000 INFO @ Thu, 12 Dec 2019 00:58:13: 1000000 INFO @ Thu, 12 Dec 2019 00:58:18: 6000000 INFO @ Thu, 12 Dec 2019 00:58:19: 11000000 INFO @ Thu, 12 Dec 2019 00:58:20: 2000000 INFO @ Thu, 12 Dec 2019 00:58:25: 7000000 INFO @ Thu, 12 Dec 2019 00:58:26: 12000000 INFO @ Thu, 12 Dec 2019 00:58:27: 3000000 INFO @ Thu, 12 Dec 2019 00:58:32: 8000000 INFO @ Thu, 12 Dec 2019 00:58:33: 13000000 INFO @ Thu, 12 Dec 2019 00:58:34: 4000000 INFO @ Thu, 12 Dec 2019 00:58:39: 9000000 INFO @ Thu, 12 Dec 2019 00:58:40: 14000000 INFO @ Thu, 12 Dec 2019 00:58:42: 5000000 INFO @ Thu, 12 Dec 2019 00:58:46: 10000000 INFO @ Thu, 12 Dec 2019 00:58:47: 15000000 INFO @ Thu, 12 Dec 2019 00:58:49: 6000000 INFO @ Thu, 12 Dec 2019 00:58:53: 11000000 INFO @ Thu, 12 Dec 2019 00:58:54: 16000000 INFO @ Thu, 12 Dec 2019 00:58:56: 7000000 INFO @ Thu, 12 Dec 2019 00:59:00: 12000000 INFO @ Thu, 12 Dec 2019 00:59:01: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:59:01: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:59:01: #1 total tags in treatment: 16971169 INFO @ Thu, 12 Dec 2019 00:59:01: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:59:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:59:01: #1 tags after filtering in treatment: 16971169 INFO @ Thu, 12 Dec 2019 00:59:01: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:59:01: #1 finished! INFO @ Thu, 12 Dec 2019 00:59:01: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:59:01: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:59:03: #2 number of paired peaks: 3 WARNING @ Thu, 12 Dec 2019 00:59:03: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:59:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:59:03: 8000000 INFO @ Thu, 12 Dec 2019 00:59:07: 13000000 INFO @ Thu, 12 Dec 2019 00:59:10: 9000000 INFO @ Thu, 12 Dec 2019 00:59:15: 14000000 INFO @ Thu, 12 Dec 2019 00:59:17: 10000000 INFO @ Thu, 12 Dec 2019 00:59:22: 15000000 INFO @ Thu, 12 Dec 2019 00:59:25: 11000000 INFO @ Thu, 12 Dec 2019 00:59:29: 16000000 INFO @ Thu, 12 Dec 2019 00:59:32: 12000000 INFO @ Thu, 12 Dec 2019 00:59:36: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:59:36: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:59:36: #1 total tags in treatment: 16971169 INFO @ Thu, 12 Dec 2019 00:59:36: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:59:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:59:36: #1 tags after filtering in treatment: 16971169 INFO @ Thu, 12 Dec 2019 00:59:36: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:59:36: #1 finished! INFO @ Thu, 12 Dec 2019 00:59:36: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:59:36: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:59:37: #2 number of paired peaks: 3 WARNING @ Thu, 12 Dec 2019 00:59:37: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:59:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:59:39: 13000000 INFO @ Thu, 12 Dec 2019 00:59:45: 14000000 INFO @ Thu, 12 Dec 2019 00:59:53: 15000000 INFO @ Thu, 12 Dec 2019 01:00:00: 16000000 INFO @ Thu, 12 Dec 2019 01:00:07: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:00:07: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:00:07: #1 total tags in treatment: 16971169 INFO @ Thu, 12 Dec 2019 01:00:07: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:00:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:00:07: #1 tags after filtering in treatment: 16971169 INFO @ Thu, 12 Dec 2019 01:00:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:00:07: #1 finished! INFO @ Thu, 12 Dec 2019 01:00:07: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:00:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:00:08: #2 number of paired peaks: 3 WARNING @ Thu, 12 Dec 2019 01:00:08: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:00:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467022/SRX467022.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。