Job ID = 4303107 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,172,213 reads read : 27,172,213 reads written : 27,172,213 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164445.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:48 27172213 reads; of these: 27172213 (100.00%) were unpaired; of these: 10537742 (38.78%) aligned 0 times 15096506 (55.56%) aligned exactly 1 time 1537965 (5.66%) aligned >1 times 61.22% overall alignment rate Time searching: 00:04:48 Overall time: 00:04:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4594279 / 16634471 = 0.2762 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 00:53:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:53:45: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:53:45: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:53:52: 1000000 INFO @ Thu, 12 Dec 2019 00:53:59: 2000000 INFO @ Thu, 12 Dec 2019 00:54:05: 3000000 INFO @ Thu, 12 Dec 2019 00:54:12: 4000000 INFO @ Thu, 12 Dec 2019 00:54:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:54:15: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:54:15: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:54:18: 5000000 INFO @ Thu, 12 Dec 2019 00:54:21: 1000000 INFO @ Thu, 12 Dec 2019 00:54:25: 6000000 INFO @ Thu, 12 Dec 2019 00:54:28: 2000000 INFO @ Thu, 12 Dec 2019 00:54:32: 7000000 INFO @ Thu, 12 Dec 2019 00:54:35: 3000000 INFO @ Thu, 12 Dec 2019 00:54:39: 8000000 INFO @ Thu, 12 Dec 2019 00:54:41: 4000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 00:54:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:54:45: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:54:45: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:54:46: 9000000 INFO @ Thu, 12 Dec 2019 00:54:48: 5000000 INFO @ Thu, 12 Dec 2019 00:54:51: 1000000 INFO @ Thu, 12 Dec 2019 00:54:53: 10000000 INFO @ Thu, 12 Dec 2019 00:54:56: 6000000 INFO @ Thu, 12 Dec 2019 00:54:58: 2000000 INFO @ Thu, 12 Dec 2019 00:55:00: 11000000 INFO @ Thu, 12 Dec 2019 00:55:02: 7000000 INFO @ Thu, 12 Dec 2019 00:55:04: 3000000 INFO @ Thu, 12 Dec 2019 00:55:06: 12000000 INFO @ Thu, 12 Dec 2019 00:55:07: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:55:07: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:55:07: #1 total tags in treatment: 12040192 INFO @ Thu, 12 Dec 2019 00:55:07: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:55:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:55:07: #1 tags after filtering in treatment: 12040192 INFO @ Thu, 12 Dec 2019 00:55:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:55:07: #1 finished! INFO @ Thu, 12 Dec 2019 00:55:07: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:55:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:55:08: #2 number of paired peaks: 56 WARNING @ Thu, 12 Dec 2019 00:55:08: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:55:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:55:09: 8000000 INFO @ Thu, 12 Dec 2019 00:55:11: 4000000 INFO @ Thu, 12 Dec 2019 00:55:15: 9000000 INFO @ Thu, 12 Dec 2019 00:55:17: 5000000 INFO @ Thu, 12 Dec 2019 00:55:21: 10000000 INFO @ Thu, 12 Dec 2019 00:55:24: 6000000 INFO @ Thu, 12 Dec 2019 00:55:27: 11000000 INFO @ Thu, 12 Dec 2019 00:55:30: 7000000 INFO @ Thu, 12 Dec 2019 00:55:34: 12000000 INFO @ Thu, 12 Dec 2019 00:55:34: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:55:34: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:55:34: #1 total tags in treatment: 12040192 INFO @ Thu, 12 Dec 2019 00:55:34: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:55:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:55:34: #1 tags after filtering in treatment: 12040192 INFO @ Thu, 12 Dec 2019 00:55:34: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:55:34: #1 finished! INFO @ Thu, 12 Dec 2019 00:55:34: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:55:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:55:35: #2 number of paired peaks: 56 WARNING @ Thu, 12 Dec 2019 00:55:35: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:55:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:55:37: 8000000 INFO @ Thu, 12 Dec 2019 00:55:43: 9000000 INFO @ Thu, 12 Dec 2019 00:55:49: 10000000 INFO @ Thu, 12 Dec 2019 00:55:56: 11000000 INFO @ Thu, 12 Dec 2019 00:56:02: 12000000 INFO @ Thu, 12 Dec 2019 00:56:02: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:56:02: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:56:02: #1 total tags in treatment: 12040192 INFO @ Thu, 12 Dec 2019 00:56:02: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:56:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:56:03: #1 tags after filtering in treatment: 12040192 INFO @ Thu, 12 Dec 2019 00:56:03: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:56:03: #1 finished! INFO @ Thu, 12 Dec 2019 00:56:03: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:56:03: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:56:03: #2 number of paired peaks: 56 WARNING @ Thu, 12 Dec 2019 00:56:03: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:56:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467021/SRX467021.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。