Job ID = 4303104 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,375,697 reads read : 28,375,697 reads written : 28,375,697 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164442.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:54 28375697 reads; of these: 28375697 (100.00%) were unpaired; of these: 1184302 (4.17%) aligned 0 times 22695084 (79.98%) aligned exactly 1 time 4496311 (15.85%) aligned >1 times 95.83% overall alignment rate Time searching: 00:07:54 Overall time: 00:07:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5882623 / 27191395 = 0.2163 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 00:58:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:58:44: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:58:44: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:58:49: 1000000 INFO @ Thu, 12 Dec 2019 00:58:55: 2000000 INFO @ Thu, 12 Dec 2019 00:59:01: 3000000 INFO @ Thu, 12 Dec 2019 00:59:06: 4000000 INFO @ Thu, 12 Dec 2019 00:59:12: 5000000 INFO @ Thu, 12 Dec 2019 00:59:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:59:13: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:59:13: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:59:18: 6000000 INFO @ Thu, 12 Dec 2019 00:59:19: 1000000 INFO @ Thu, 12 Dec 2019 00:59:24: 7000000 INFO @ Thu, 12 Dec 2019 00:59:24: 2000000 INFO @ Thu, 12 Dec 2019 00:59:30: 8000000 INFO @ Thu, 12 Dec 2019 00:59:30: 3000000 INFO @ Thu, 12 Dec 2019 00:59:35: 4000000 INFO @ Thu, 12 Dec 2019 00:59:36: 9000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 00:59:41: 5000000 INFO @ Thu, 12 Dec 2019 00:59:41: 10000000 INFO @ Thu, 12 Dec 2019 00:59:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:59:43: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:59:43: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:59:47: 6000000 INFO @ Thu, 12 Dec 2019 00:59:47: 11000000 INFO @ Thu, 12 Dec 2019 00:59:49: 1000000 INFO @ Thu, 12 Dec 2019 00:59:53: 12000000 INFO @ Thu, 12 Dec 2019 00:59:53: 7000000 INFO @ Thu, 12 Dec 2019 00:59:55: 2000000 INFO @ Thu, 12 Dec 2019 00:59:59: 13000000 INFO @ Thu, 12 Dec 2019 01:00:00: 8000000 INFO @ Thu, 12 Dec 2019 01:00:01: 3000000 INFO @ Thu, 12 Dec 2019 01:00:05: 14000000 INFO @ Thu, 12 Dec 2019 01:00:06: 9000000 INFO @ Thu, 12 Dec 2019 01:00:06: 4000000 INFO @ Thu, 12 Dec 2019 01:00:10: 15000000 INFO @ Thu, 12 Dec 2019 01:00:12: 5000000 INFO @ Thu, 12 Dec 2019 01:00:13: 10000000 INFO @ Thu, 12 Dec 2019 01:00:16: 16000000 INFO @ Thu, 12 Dec 2019 01:00:18: 6000000 INFO @ Thu, 12 Dec 2019 01:00:19: 11000000 INFO @ Thu, 12 Dec 2019 01:00:22: 17000000 INFO @ Thu, 12 Dec 2019 01:00:24: 7000000 INFO @ Thu, 12 Dec 2019 01:00:25: 12000000 INFO @ Thu, 12 Dec 2019 01:00:28: 18000000 INFO @ Thu, 12 Dec 2019 01:00:30: 8000000 INFO @ Thu, 12 Dec 2019 01:00:31: 13000000 INFO @ Thu, 12 Dec 2019 01:00:34: 19000000 INFO @ Thu, 12 Dec 2019 01:00:36: 9000000 INFO @ Thu, 12 Dec 2019 01:00:38: 14000000 INFO @ Thu, 12 Dec 2019 01:00:40: 20000000 INFO @ Thu, 12 Dec 2019 01:00:41: 10000000 INFO @ Thu, 12 Dec 2019 01:00:44: 15000000 INFO @ Thu, 12 Dec 2019 01:00:46: 21000000 INFO @ Thu, 12 Dec 2019 01:00:47: 11000000 INFO @ Thu, 12 Dec 2019 01:00:48: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:00:48: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:00:48: #1 total tags in treatment: 21308772 INFO @ Thu, 12 Dec 2019 01:00:48: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:00:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:00:49: #1 tags after filtering in treatment: 21308772 INFO @ Thu, 12 Dec 2019 01:00:49: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:00:49: #1 finished! INFO @ Thu, 12 Dec 2019 01:00:49: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:00:49: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:00:50: #2 number of paired peaks: 1 WARNING @ Thu, 12 Dec 2019 01:00:50: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:00:50: Process for pairing-model is terminated! INFO @ Thu, 12 Dec 2019 01:00:50: 16000000 cut: /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:00:53: 12000000 INFO @ Thu, 12 Dec 2019 01:00:56: 17000000 INFO @ Thu, 12 Dec 2019 01:00:58: 13000000 INFO @ Thu, 12 Dec 2019 01:01:02: 18000000 INFO @ Thu, 12 Dec 2019 01:01:04: 14000000 INFO @ Thu, 12 Dec 2019 01:01:08: 19000000 INFO @ Thu, 12 Dec 2019 01:01:09: 15000000 INFO @ Thu, 12 Dec 2019 01:01:14: 20000000 INFO @ Thu, 12 Dec 2019 01:01:15: 16000000 INFO @ Thu, 12 Dec 2019 01:01:20: 21000000 INFO @ Thu, 12 Dec 2019 01:01:21: 17000000 INFO @ Thu, 12 Dec 2019 01:01:22: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:01:22: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:01:22: #1 total tags in treatment: 21308772 INFO @ Thu, 12 Dec 2019 01:01:22: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:01:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:01:22: #1 tags after filtering in treatment: 21308772 INFO @ Thu, 12 Dec 2019 01:01:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:01:22: #1 finished! INFO @ Thu, 12 Dec 2019 01:01:22: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:01:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:01:24: #2 number of paired peaks: 1 WARNING @ Thu, 12 Dec 2019 01:01:24: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:01:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:01:26: 18000000 INFO @ Thu, 12 Dec 2019 01:01:31: 19000000 INFO @ Thu, 12 Dec 2019 01:01:36: 20000000 INFO @ Thu, 12 Dec 2019 01:01:41: 21000000 INFO @ Thu, 12 Dec 2019 01:01:43: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:01:43: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:01:43: #1 total tags in treatment: 21308772 INFO @ Thu, 12 Dec 2019 01:01:43: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:01:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:01:43: #1 tags after filtering in treatment: 21308772 INFO @ Thu, 12 Dec 2019 01:01:43: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:01:43: #1 finished! INFO @ Thu, 12 Dec 2019 01:01:43: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:01:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:01:44: #2 number of paired peaks: 1 WARNING @ Thu, 12 Dec 2019 01:01:44: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:01:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467018/SRX467018.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。