Job ID = 4303102 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 43,059,388 reads read : 43,059,388 reads written : 43,059,388 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164440.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:28 43059388 reads; of these: 43059388 (100.00%) were unpaired; of these: 1870780 (4.34%) aligned 0 times 34205795 (79.44%) aligned exactly 1 time 6982813 (16.22%) aligned >1 times 95.66% overall alignment rate Time searching: 00:15:28 Overall time: 00:15:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11593684 / 41188608 = 0.2815 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:12:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:12:21: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:12:21: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:12:31: 1000000 INFO @ Thu, 12 Dec 2019 01:12:42: 2000000 INFO @ Thu, 12 Dec 2019 01:12:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:12:51: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:12:51: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:12:51: 3000000 INFO @ Thu, 12 Dec 2019 01:13:01: 4000000 INFO @ Thu, 12 Dec 2019 01:13:01: 1000000 INFO @ Thu, 12 Dec 2019 01:13:10: 5000000 INFO @ Thu, 12 Dec 2019 01:13:11: 2000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:13:20: 6000000 INFO @ Thu, 12 Dec 2019 01:13:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:13:21: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:13:21: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:13:22: 3000000 INFO @ Thu, 12 Dec 2019 01:13:30: 7000000 INFO @ Thu, 12 Dec 2019 01:13:31: 1000000 INFO @ Thu, 12 Dec 2019 01:13:32: 4000000 INFO @ Thu, 12 Dec 2019 01:13:40: 8000000 INFO @ Thu, 12 Dec 2019 01:13:41: 2000000 INFO @ Thu, 12 Dec 2019 01:13:42: 5000000 INFO @ Thu, 12 Dec 2019 01:13:50: 9000000 INFO @ Thu, 12 Dec 2019 01:13:52: 3000000 INFO @ Thu, 12 Dec 2019 01:13:53: 6000000 INFO @ Thu, 12 Dec 2019 01:14:00: 10000000 INFO @ Thu, 12 Dec 2019 01:14:03: 7000000 INFO @ Thu, 12 Dec 2019 01:14:04: 4000000 INFO @ Thu, 12 Dec 2019 01:14:09: 11000000 INFO @ Thu, 12 Dec 2019 01:14:14: 8000000 INFO @ Thu, 12 Dec 2019 01:14:14: 5000000 INFO @ Thu, 12 Dec 2019 01:14:19: 12000000 INFO @ Thu, 12 Dec 2019 01:14:24: 6000000 INFO @ Thu, 12 Dec 2019 01:14:24: 9000000 INFO @ Thu, 12 Dec 2019 01:14:29: 13000000 INFO @ Thu, 12 Dec 2019 01:14:34: 7000000 INFO @ Thu, 12 Dec 2019 01:14:35: 10000000 INFO @ Thu, 12 Dec 2019 01:14:38: 14000000 INFO @ Thu, 12 Dec 2019 01:14:44: 8000000 INFO @ Thu, 12 Dec 2019 01:14:46: 11000000 INFO @ Thu, 12 Dec 2019 01:14:48: 15000000 INFO @ Thu, 12 Dec 2019 01:14:54: 9000000 INFO @ Thu, 12 Dec 2019 01:14:56: 12000000 INFO @ Thu, 12 Dec 2019 01:14:57: 16000000 INFO @ Thu, 12 Dec 2019 01:15:04: 10000000 INFO @ Thu, 12 Dec 2019 01:15:06: 13000000 INFO @ Thu, 12 Dec 2019 01:15:07: 17000000 INFO @ Thu, 12 Dec 2019 01:15:14: 11000000 INFO @ Thu, 12 Dec 2019 01:15:15: 18000000 INFO @ Thu, 12 Dec 2019 01:15:16: 14000000 INFO @ Thu, 12 Dec 2019 01:15:23: 19000000 INFO @ Thu, 12 Dec 2019 01:15:24: 12000000 INFO @ Thu, 12 Dec 2019 01:15:26: 15000000 INFO @ Thu, 12 Dec 2019 01:15:31: 20000000 INFO @ Thu, 12 Dec 2019 01:15:34: 13000000 INFO @ Thu, 12 Dec 2019 01:15:36: 16000000 INFO @ Thu, 12 Dec 2019 01:15:39: 21000000 INFO @ Thu, 12 Dec 2019 01:15:43: 14000000 INFO @ Thu, 12 Dec 2019 01:15:46: 17000000 INFO @ Thu, 12 Dec 2019 01:15:47: 22000000 INFO @ Thu, 12 Dec 2019 01:15:53: 15000000 INFO @ Thu, 12 Dec 2019 01:15:55: 23000000 INFO @ Thu, 12 Dec 2019 01:15:56: 18000000 INFO @ Thu, 12 Dec 2019 01:16:02: 16000000 INFO @ Thu, 12 Dec 2019 01:16:03: 24000000 INFO @ Thu, 12 Dec 2019 01:16:06: 19000000 INFO @ Thu, 12 Dec 2019 01:16:11: 25000000 INFO @ Thu, 12 Dec 2019 01:16:12: 17000000 INFO @ Thu, 12 Dec 2019 01:16:17: 20000000 INFO @ Thu, 12 Dec 2019 01:16:19: 26000000 INFO @ Thu, 12 Dec 2019 01:16:22: 18000000 INFO @ Thu, 12 Dec 2019 01:16:27: 27000000 INFO @ Thu, 12 Dec 2019 01:16:27: 21000000 INFO @ Thu, 12 Dec 2019 01:16:31: 19000000 INFO @ Thu, 12 Dec 2019 01:16:35: 28000000 INFO @ Thu, 12 Dec 2019 01:16:37: 22000000 INFO @ Thu, 12 Dec 2019 01:16:41: 20000000 INFO @ Thu, 12 Dec 2019 01:16:43: 29000000 INFO @ Thu, 12 Dec 2019 01:16:46: 23000000 INFO @ Thu, 12 Dec 2019 01:16:48: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:16:48: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:16:48: #1 total tags in treatment: 29594924 INFO @ Thu, 12 Dec 2019 01:16:48: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:16:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:16:48: #1 tags after filtering in treatment: 29594924 INFO @ Thu, 12 Dec 2019 01:16:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:16:48: #1 finished! INFO @ Thu, 12 Dec 2019 01:16:48: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:16:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:16:51: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:16:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:16:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:16:51: 21000000 INFO @ Thu, 12 Dec 2019 01:16:56: 24000000 INFO @ Thu, 12 Dec 2019 01:17:01: 22000000 INFO @ Thu, 12 Dec 2019 01:17:06: 25000000 INFO @ Thu, 12 Dec 2019 01:17:11: 23000000 INFO @ Thu, 12 Dec 2019 01:17:15: 26000000 INFO @ Thu, 12 Dec 2019 01:17:20: 24000000 INFO @ Thu, 12 Dec 2019 01:17:25: 27000000 INFO @ Thu, 12 Dec 2019 01:17:30: 25000000 INFO @ Thu, 12 Dec 2019 01:17:34: 28000000 INFO @ Thu, 12 Dec 2019 01:17:39: 26000000 INFO @ Thu, 12 Dec 2019 01:17:44: 29000000 INFO @ Thu, 12 Dec 2019 01:17:49: 27000000 INFO @ Thu, 12 Dec 2019 01:17:49: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:17:49: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:17:49: #1 total tags in treatment: 29594924 INFO @ Thu, 12 Dec 2019 01:17:49: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:17:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:17:50: #1 tags after filtering in treatment: 29594924 INFO @ Thu, 12 Dec 2019 01:17:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:17:50: #1 finished! INFO @ Thu, 12 Dec 2019 01:17:50: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:17:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:17:52: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:17:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:17:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:17:58: 28000000 INFO @ Thu, 12 Dec 2019 01:18:07: 29000000 INFO @ Thu, 12 Dec 2019 01:18:13: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:18:13: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:18:13: #1 total tags in treatment: 29594924 INFO @ Thu, 12 Dec 2019 01:18:13: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:18:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:18:14: #1 tags after filtering in treatment: 29594924 INFO @ Thu, 12 Dec 2019 01:18:14: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:18:14: #1 finished! INFO @ Thu, 12 Dec 2019 01:18:14: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:18:14: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:18:16: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:18:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:18:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467016/SRX467016.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。