Job ID = 4303101 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 43,059,388 reads read : 43,059,388 reads written : 43,059,388 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164439.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:12 43059388 reads; of these: 43059388 (100.00%) were unpaired; of these: 1870849 (4.34%) aligned 0 times 34205747 (79.44%) aligned exactly 1 time 6982792 (16.22%) aligned >1 times 95.66% overall alignment rate Time searching: 00:10:13 Overall time: 00:10:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11592545 / 41188539 = 0.2815 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:02:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:02:48: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:02:48: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:02:52: 1000000 INFO @ Thu, 12 Dec 2019 01:02:57: 2000000 INFO @ Thu, 12 Dec 2019 01:03:01: 3000000 INFO @ Thu, 12 Dec 2019 01:03:06: 4000000 INFO @ Thu, 12 Dec 2019 01:03:10: 5000000 INFO @ Thu, 12 Dec 2019 01:03:14: 6000000 INFO @ Thu, 12 Dec 2019 01:03:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:03:17: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:03:17: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:03:19: 7000000 INFO @ Thu, 12 Dec 2019 01:03:21: 1000000 INFO @ Thu, 12 Dec 2019 01:03:23: 8000000 INFO @ Thu, 12 Dec 2019 01:03:26: 2000000 INFO @ Thu, 12 Dec 2019 01:03:28: 9000000 INFO @ Thu, 12 Dec 2019 01:03:31: 3000000 INFO @ Thu, 12 Dec 2019 01:03:32: 10000000 INFO @ Thu, 12 Dec 2019 01:03:35: 4000000 INFO @ Thu, 12 Dec 2019 01:03:37: 11000000 INFO @ Thu, 12 Dec 2019 01:03:40: 5000000 INFO @ Thu, 12 Dec 2019 01:03:42: 12000000 INFO @ Thu, 12 Dec 2019 01:03:44: 6000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:03:46: 13000000 INFO @ Thu, 12 Dec 2019 01:03:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:03:47: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:03:47: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:03:49: 7000000 INFO @ Thu, 12 Dec 2019 01:03:51: 14000000 INFO @ Thu, 12 Dec 2019 01:03:52: 1000000 INFO @ Thu, 12 Dec 2019 01:03:53: 8000000 INFO @ Thu, 12 Dec 2019 01:03:56: 15000000 INFO @ Thu, 12 Dec 2019 01:03:56: 2000000 INFO @ Thu, 12 Dec 2019 01:03:58: 9000000 INFO @ Thu, 12 Dec 2019 01:04:01: 16000000 INFO @ Thu, 12 Dec 2019 01:04:01: 3000000 INFO @ Thu, 12 Dec 2019 01:04:02: 10000000 INFO @ Thu, 12 Dec 2019 01:04:06: 17000000 INFO @ Thu, 12 Dec 2019 01:04:06: 11000000 INFO @ Thu, 12 Dec 2019 01:04:07: 4000000 INFO @ Thu, 12 Dec 2019 01:04:11: 18000000 INFO @ Thu, 12 Dec 2019 01:04:11: 12000000 INFO @ Thu, 12 Dec 2019 01:04:11: 5000000 INFO @ Thu, 12 Dec 2019 01:04:15: 13000000 INFO @ Thu, 12 Dec 2019 01:04:16: 19000000 INFO @ Thu, 12 Dec 2019 01:04:16: 6000000 INFO @ Thu, 12 Dec 2019 01:04:20: 14000000 INFO @ Thu, 12 Dec 2019 01:04:20: 20000000 INFO @ Thu, 12 Dec 2019 01:04:21: 7000000 INFO @ Thu, 12 Dec 2019 01:04:24: 15000000 INFO @ Thu, 12 Dec 2019 01:04:25: 21000000 INFO @ Thu, 12 Dec 2019 01:04:25: 8000000 INFO @ Thu, 12 Dec 2019 01:04:29: 16000000 INFO @ Thu, 12 Dec 2019 01:04:30: 22000000 INFO @ Thu, 12 Dec 2019 01:04:30: 9000000 INFO @ Thu, 12 Dec 2019 01:04:33: 17000000 INFO @ Thu, 12 Dec 2019 01:04:35: 23000000 INFO @ Thu, 12 Dec 2019 01:04:35: 10000000 INFO @ Thu, 12 Dec 2019 01:04:37: 18000000 INFO @ Thu, 12 Dec 2019 01:04:40: 11000000 INFO @ Thu, 12 Dec 2019 01:04:40: 24000000 INFO @ Thu, 12 Dec 2019 01:04:42: 19000000 INFO @ Thu, 12 Dec 2019 01:04:44: 25000000 INFO @ Thu, 12 Dec 2019 01:04:45: 12000000 INFO @ Thu, 12 Dec 2019 01:04:46: 20000000 INFO @ Thu, 12 Dec 2019 01:04:49: 26000000 INFO @ Thu, 12 Dec 2019 01:04:50: 13000000 INFO @ Thu, 12 Dec 2019 01:04:51: 21000000 INFO @ Thu, 12 Dec 2019 01:04:54: 27000000 INFO @ Thu, 12 Dec 2019 01:04:55: 14000000 INFO @ Thu, 12 Dec 2019 01:04:55: 22000000 INFO @ Thu, 12 Dec 2019 01:04:59: 28000000 INFO @ Thu, 12 Dec 2019 01:05:00: 23000000 INFO @ Thu, 12 Dec 2019 01:05:00: 15000000 INFO @ Thu, 12 Dec 2019 01:05:03: 29000000 INFO @ Thu, 12 Dec 2019 01:05:04: 24000000 INFO @ Thu, 12 Dec 2019 01:05:05: 16000000 INFO @ Thu, 12 Dec 2019 01:05:06: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:05:06: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:05:06: #1 total tags in treatment: 29595994 INFO @ Thu, 12 Dec 2019 01:05:06: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:05:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:05:07: #1 tags after filtering in treatment: 29595994 INFO @ Thu, 12 Dec 2019 01:05:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:05:07: #1 finished! INFO @ Thu, 12 Dec 2019 01:05:07: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:05:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:05:08: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:05:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:05:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.05_peaks.narrowPeak: No such file or directory INFO @ Thu, 12 Dec 2019 01:05:08: 25000000 pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:05:09: 17000000 INFO @ Thu, 12 Dec 2019 01:05:13: 26000000 INFO @ Thu, 12 Dec 2019 01:05:14: 18000000 INFO @ Thu, 12 Dec 2019 01:05:17: 27000000 INFO @ Thu, 12 Dec 2019 01:05:18: 19000000 INFO @ Thu, 12 Dec 2019 01:05:22: 28000000 INFO @ Thu, 12 Dec 2019 01:05:22: 20000000 INFO @ Thu, 12 Dec 2019 01:05:26: 29000000 INFO @ Thu, 12 Dec 2019 01:05:27: 21000000 INFO @ Thu, 12 Dec 2019 01:05:29: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:05:29: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:05:29: #1 total tags in treatment: 29595994 INFO @ Thu, 12 Dec 2019 01:05:29: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:05:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:05:29: #1 tags after filtering in treatment: 29595994 INFO @ Thu, 12 Dec 2019 01:05:29: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:05:29: #1 finished! INFO @ Thu, 12 Dec 2019 01:05:29: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:05:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:05:31: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:05:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:05:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:05:31: 22000000 INFO @ Thu, 12 Dec 2019 01:05:35: 23000000 INFO @ Thu, 12 Dec 2019 01:05:40: 24000000 INFO @ Thu, 12 Dec 2019 01:05:44: 25000000 INFO @ Thu, 12 Dec 2019 01:05:49: 26000000 INFO @ Thu, 12 Dec 2019 01:05:53: 27000000 INFO @ Thu, 12 Dec 2019 01:05:58: 28000000 INFO @ Thu, 12 Dec 2019 01:06:02: 29000000 INFO @ Thu, 12 Dec 2019 01:06:05: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:06:05: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:06:05: #1 total tags in treatment: 29595994 INFO @ Thu, 12 Dec 2019 01:06:05: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:06:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:06:06: #1 tags after filtering in treatment: 29595994 INFO @ Thu, 12 Dec 2019 01:06:06: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:06:06: #1 finished! INFO @ Thu, 12 Dec 2019 01:06:06: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:06:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:06:07: #2 number of paired peaks: 0 WARNING @ Thu, 12 Dec 2019 01:06:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:06:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467015/SRX467015.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。