Job ID = 4303099 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 60,880,063 reads read : 60,880,063 reads written : 60,880,063 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164437.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:26 60880063 reads; of these: 60880063 (100.00%) were unpaired; of these: 27069326 (44.46%) aligned 0 times 30093772 (49.43%) aligned exactly 1 time 3716965 (6.11%) aligned >1 times 55.54% overall alignment rate Time searching: 00:11:26 Overall time: 00:11:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11221220 / 33810737 = 0.3319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:05:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:05:56: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:05:56: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:06:06: 1000000 INFO @ Thu, 12 Dec 2019 01:06:16: 2000000 INFO @ Thu, 12 Dec 2019 01:06:25: 3000000 INFO @ Thu, 12 Dec 2019 01:06:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:06:26: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:06:26: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:06:34: 1000000 INFO @ Thu, 12 Dec 2019 01:06:35: 4000000 INFO @ Thu, 12 Dec 2019 01:06:41: 2000000 INFO @ Thu, 12 Dec 2019 01:06:44: 5000000 INFO @ Thu, 12 Dec 2019 01:06:48: 3000000 INFO @ Thu, 12 Dec 2019 01:06:54: 6000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:06:55: 4000000 INFO @ Thu, 12 Dec 2019 01:06:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:06:56: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:06:56: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:07:03: 5000000 INFO @ Thu, 12 Dec 2019 01:07:03: 7000000 INFO @ Thu, 12 Dec 2019 01:07:05: 1000000 INFO @ Thu, 12 Dec 2019 01:07:10: 6000000 INFO @ Thu, 12 Dec 2019 01:07:12: 8000000 INFO @ Thu, 12 Dec 2019 01:07:13: 2000000 INFO @ Thu, 12 Dec 2019 01:07:17: 7000000 INFO @ Thu, 12 Dec 2019 01:07:22: 3000000 INFO @ Thu, 12 Dec 2019 01:07:22: 9000000 INFO @ Thu, 12 Dec 2019 01:07:25: 8000000 INFO @ Thu, 12 Dec 2019 01:07:30: 4000000 INFO @ Thu, 12 Dec 2019 01:07:31: 10000000 INFO @ Thu, 12 Dec 2019 01:07:32: 9000000 INFO @ Thu, 12 Dec 2019 01:07:38: 5000000 INFO @ Thu, 12 Dec 2019 01:07:39: 10000000 INFO @ Thu, 12 Dec 2019 01:07:41: 11000000 INFO @ Thu, 12 Dec 2019 01:07:46: 6000000 INFO @ Thu, 12 Dec 2019 01:07:46: 11000000 INFO @ Thu, 12 Dec 2019 01:07:50: 12000000 INFO @ Thu, 12 Dec 2019 01:07:54: 12000000 INFO @ Thu, 12 Dec 2019 01:07:54: 7000000 INFO @ Thu, 12 Dec 2019 01:07:59: 13000000 INFO @ Thu, 12 Dec 2019 01:08:01: 13000000 INFO @ Thu, 12 Dec 2019 01:08:03: 8000000 INFO @ Thu, 12 Dec 2019 01:08:08: 14000000 INFO @ Thu, 12 Dec 2019 01:08:09: 14000000 INFO @ Thu, 12 Dec 2019 01:08:11: 9000000 INFO @ Thu, 12 Dec 2019 01:08:16: 15000000 INFO @ Thu, 12 Dec 2019 01:08:18: 15000000 INFO @ Thu, 12 Dec 2019 01:08:19: 10000000 INFO @ Thu, 12 Dec 2019 01:08:23: 16000000 INFO @ Thu, 12 Dec 2019 01:08:27: 11000000 INFO @ Thu, 12 Dec 2019 01:08:27: 16000000 INFO @ Thu, 12 Dec 2019 01:08:30: 17000000 INFO @ Thu, 12 Dec 2019 01:08:35: 12000000 INFO @ Thu, 12 Dec 2019 01:08:37: 17000000 INFO @ Thu, 12 Dec 2019 01:08:38: 18000000 INFO @ Thu, 12 Dec 2019 01:08:43: 13000000 INFO @ Thu, 12 Dec 2019 01:08:45: 19000000 INFO @ Thu, 12 Dec 2019 01:08:46: 18000000 INFO @ Thu, 12 Dec 2019 01:08:51: 14000000 INFO @ Thu, 12 Dec 2019 01:08:52: 20000000 INFO @ Thu, 12 Dec 2019 01:08:56: 19000000 INFO @ Thu, 12 Dec 2019 01:08:59: 15000000 INFO @ Thu, 12 Dec 2019 01:08:59: 21000000 INFO @ Thu, 12 Dec 2019 01:09:05: 20000000 INFO @ Thu, 12 Dec 2019 01:09:07: 22000000 INFO @ Thu, 12 Dec 2019 01:09:07: 16000000 INFO @ Thu, 12 Dec 2019 01:09:11: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:09:11: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:09:11: #1 total tags in treatment: 22589517 INFO @ Thu, 12 Dec 2019 01:09:11: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:09:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:09:12: #1 tags after filtering in treatment: 22589517 INFO @ Thu, 12 Dec 2019 01:09:12: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:09:12: #1 finished! INFO @ Thu, 12 Dec 2019 01:09:12: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:09:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:09:14: #2 number of paired peaks: 4 WARNING @ Thu, 12 Dec 2019 01:09:14: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:09:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:09:15: 21000000 INFO @ Thu, 12 Dec 2019 01:09:15: 17000000 INFO @ Thu, 12 Dec 2019 01:09:24: 18000000 INFO @ Thu, 12 Dec 2019 01:09:24: 22000000 INFO @ Thu, 12 Dec 2019 01:09:29: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:09:29: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:09:29: #1 total tags in treatment: 22589517 INFO @ Thu, 12 Dec 2019 01:09:29: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:09:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:09:30: #1 tags after filtering in treatment: 22589517 INFO @ Thu, 12 Dec 2019 01:09:30: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:09:30: #1 finished! INFO @ Thu, 12 Dec 2019 01:09:30: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:09:30: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:09:32: #2 number of paired peaks: 4 WARNING @ Thu, 12 Dec 2019 01:09:32: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:09:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:09:32: 19000000 INFO @ Thu, 12 Dec 2019 01:09:40: 20000000 INFO @ Thu, 12 Dec 2019 01:09:47: 21000000 INFO @ Thu, 12 Dec 2019 01:09:55: 22000000 INFO @ Thu, 12 Dec 2019 01:10:00: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:10:00: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:10:00: #1 total tags in treatment: 22589517 INFO @ Thu, 12 Dec 2019 01:10:00: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:10:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:10:00: #1 tags after filtering in treatment: 22589517 INFO @ Thu, 12 Dec 2019 01:10:00: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:10:00: #1 finished! INFO @ Thu, 12 Dec 2019 01:10:00: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:10:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:10:02: #2 number of paired peaks: 4 WARNING @ Thu, 12 Dec 2019 01:10:02: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:10:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467013/SRX467013.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。