Job ID = 4303097 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,374,320 reads read : 17,374,320 reads written : 17,374,320 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1164435.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:12 17374320 reads; of these: 17374320 (100.00%) were unpaired; of these: 1270979 (7.32%) aligned 0 times 13922587 (80.13%) aligned exactly 1 time 2180754 (12.55%) aligned >1 times 92.68% overall alignment rate Time searching: 00:05:13 Overall time: 00:05:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2452985 / 16103341 = 0.1523 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 00:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:52:10: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:52:10: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:52:17: 1000000 INFO @ Thu, 12 Dec 2019 00:52:24: 2000000 INFO @ Thu, 12 Dec 2019 00:52:31: 3000000 INFO @ Thu, 12 Dec 2019 00:52:38: 4000000 INFO @ Thu, 12 Dec 2019 00:52:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:52:40: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:52:40: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:52:45: 5000000 INFO @ Thu, 12 Dec 2019 00:52:51: 1000000 INFO @ Thu, 12 Dec 2019 00:52:53: 6000000 INFO @ Thu, 12 Dec 2019 00:52:59: 2000000 INFO @ Thu, 12 Dec 2019 00:53:01: 7000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 00:53:08: 3000000 INFO @ Thu, 12 Dec 2019 00:53:10: 8000000 INFO @ Thu, 12 Dec 2019 00:53:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 00:53:11: #1 read tag files... INFO @ Thu, 12 Dec 2019 00:53:11: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 00:53:17: 4000000 INFO @ Thu, 12 Dec 2019 00:53:19: 9000000 INFO @ Thu, 12 Dec 2019 00:53:20: 1000000 INFO @ Thu, 12 Dec 2019 00:53:26: 5000000 INFO @ Thu, 12 Dec 2019 00:53:27: 10000000 INFO @ Thu, 12 Dec 2019 00:53:29: 2000000 INFO @ Thu, 12 Dec 2019 00:53:36: 6000000 INFO @ Thu, 12 Dec 2019 00:53:36: 11000000 INFO @ Thu, 12 Dec 2019 00:53:38: 3000000 INFO @ Thu, 12 Dec 2019 00:53:45: 12000000 INFO @ Thu, 12 Dec 2019 00:53:46: 7000000 INFO @ Thu, 12 Dec 2019 00:53:49: 4000000 INFO @ Thu, 12 Dec 2019 00:53:55: 13000000 INFO @ Thu, 12 Dec 2019 00:53:57: 8000000 INFO @ Thu, 12 Dec 2019 00:53:58: 5000000 INFO @ Thu, 12 Dec 2019 00:54:00: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:54:00: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:54:00: #1 total tags in treatment: 13650356 INFO @ Thu, 12 Dec 2019 00:54:00: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:54:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:54:00: #1 tags after filtering in treatment: 13650356 INFO @ Thu, 12 Dec 2019 00:54:00: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:54:00: #1 finished! INFO @ Thu, 12 Dec 2019 00:54:00: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:54:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:54:02: #2 number of paired peaks: 41 WARNING @ Thu, 12 Dec 2019 00:54:02: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:54:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:54:06: 6000000 INFO @ Thu, 12 Dec 2019 00:54:07: 9000000 INFO @ Thu, 12 Dec 2019 00:54:14: 7000000 INFO @ Thu, 12 Dec 2019 00:54:16: 10000000 INFO @ Thu, 12 Dec 2019 00:54:22: 8000000 INFO @ Thu, 12 Dec 2019 00:54:25: 11000000 INFO @ Thu, 12 Dec 2019 00:54:29: 9000000 INFO @ Thu, 12 Dec 2019 00:54:34: 12000000 INFO @ Thu, 12 Dec 2019 00:54:37: 10000000 INFO @ Thu, 12 Dec 2019 00:54:43: 13000000 INFO @ Thu, 12 Dec 2019 00:54:45: 11000000 INFO @ Thu, 12 Dec 2019 00:54:48: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:54:48: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:54:48: #1 total tags in treatment: 13650356 INFO @ Thu, 12 Dec 2019 00:54:48: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:54:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:54:49: #1 tags after filtering in treatment: 13650356 INFO @ Thu, 12 Dec 2019 00:54:49: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:54:49: #1 finished! INFO @ Thu, 12 Dec 2019 00:54:49: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:54:49: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:54:50: #2 number of paired peaks: 41 WARNING @ Thu, 12 Dec 2019 00:54:50: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:54:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 00:54:53: 12000000 INFO @ Thu, 12 Dec 2019 00:55:00: 13000000 INFO @ Thu, 12 Dec 2019 00:55:05: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 00:55:05: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 00:55:05: #1 total tags in treatment: 13650356 INFO @ Thu, 12 Dec 2019 00:55:05: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 00:55:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 00:55:06: #1 tags after filtering in treatment: 13650356 INFO @ Thu, 12 Dec 2019 00:55:06: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 00:55:06: #1 finished! INFO @ Thu, 12 Dec 2019 00:55:06: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 00:55:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 00:55:07: #2 number of paired peaks: 41 WARNING @ Thu, 12 Dec 2019 00:55:07: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 00:55:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX467011/SRX467011.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。