Job ID = 11240785 sra ファイルのダウンロード中... Completed: 494522K bytes transferred in 8 seconds (494468K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 29334226 spots for /home/okishinya/chipatlas/results/dm3/SRX4669069/SRR7817594.sra Written 29334226 spots for /home/okishinya/chipatlas/results/dm3/SRX4669069/SRR7817594.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:02 29334226 reads; of these: 29334226 (100.00%) were unpaired; of these: 1214631 (4.14%) aligned 0 times 24016862 (81.87%) aligned exactly 1 time 4102733 (13.99%) aligned >1 times 95.86% overall alignment rate Time searching: 00:08:02 Overall time: 00:08:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6952911 / 28119595 = 0.2473 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 20:51:40: # Command line: callpeak -t SRX4669069.bam -f BAM -g dm -n SRX4669069.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4669069.20 # format = BAM # ChIP-seq file = ['SRX4669069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:51:40: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:51:40: # Command line: callpeak -t SRX4669069.bam -f BAM -g dm -n SRX4669069.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4669069.05 # format = BAM # ChIP-seq file = ['SRX4669069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:51:40: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:51:40: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:51:40: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:51:40: # Command line: callpeak -t SRX4669069.bam -f BAM -g dm -n SRX4669069.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4669069.10 # format = BAM # ChIP-seq file = ['SRX4669069.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 20:51:40: #1 read tag files... INFO @ Sun, 07 Oct 2018 20:51:40: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 20:51:47: 1000000 INFO @ Sun, 07 Oct 2018 20:51:47: 1000000 INFO @ Sun, 07 Oct 2018 20:51:47: 1000000 INFO @ Sun, 07 Oct 2018 20:51:54: 2000000 INFO @ Sun, 07 Oct 2018 20:51:55: 2000000 INFO @ Sun, 07 Oct 2018 20:51:55: 2000000 INFO @ Sun, 07 Oct 2018 20:52:00: 3000000 INFO @ Sun, 07 Oct 2018 20:52:02: 3000000 INFO @ Sun, 07 Oct 2018 20:52:03: 3000000 INFO @ Sun, 07 Oct 2018 20:52:07: 4000000 INFO @ Sun, 07 Oct 2018 20:52:10: 4000000 INFO @ Sun, 07 Oct 2018 20:52:10: 4000000 INFO @ Sun, 07 Oct 2018 20:52:13: 5000000 INFO @ Sun, 07 Oct 2018 20:52:17: 5000000 INFO @ Sun, 07 Oct 2018 20:52:18: 5000000 INFO @ Sun, 07 Oct 2018 20:52:20: 6000000 INFO @ Sun, 07 Oct 2018 20:52:24: 6000000 INFO @ Sun, 07 Oct 2018 20:52:26: 6000000 INFO @ Sun, 07 Oct 2018 20:52:27: 7000000 INFO @ Sun, 07 Oct 2018 20:52:31: 7000000 INFO @ Sun, 07 Oct 2018 20:52:33: 8000000 INFO @ Sun, 07 Oct 2018 20:52:33: 7000000 INFO @ Sun, 07 Oct 2018 20:52:39: 8000000 INFO @ Sun, 07 Oct 2018 20:52:40: 9000000 INFO @ Sun, 07 Oct 2018 20:52:41: 8000000 INFO @ Sun, 07 Oct 2018 20:52:46: 9000000 INFO @ Sun, 07 Oct 2018 20:52:46: 10000000 INFO @ Sun, 07 Oct 2018 20:52:49: 9000000 INFO @ Sun, 07 Oct 2018 20:52:53: 11000000 INFO @ Sun, 07 Oct 2018 20:52:54: 10000000 INFO @ Sun, 07 Oct 2018 20:52:57: 10000000 INFO @ Sun, 07 Oct 2018 20:53:00: 12000000 INFO @ Sun, 07 Oct 2018 20:53:01: 11000000 INFO @ Sun, 07 Oct 2018 20:53:04: 11000000 INFO @ Sun, 07 Oct 2018 20:53:07: 13000000 INFO @ Sun, 07 Oct 2018 20:53:08: 12000000 INFO @ Sun, 07 Oct 2018 20:53:12: 12000000 INFO @ Sun, 07 Oct 2018 20:53:14: 14000000 INFO @ Sun, 07 Oct 2018 20:53:14: 13000000 INFO @ Sun, 07 Oct 2018 20:53:20: 13000000 INFO @ Sun, 07 Oct 2018 20:53:21: 15000000 INFO @ Sun, 07 Oct 2018 20:53:21: 14000000 INFO @ Sun, 07 Oct 2018 20:53:27: 14000000 INFO @ Sun, 07 Oct 2018 20:53:28: 16000000 INFO @ Sun, 07 Oct 2018 20:53:28: 15000000 INFO @ Sun, 07 Oct 2018 20:53:35: 17000000 INFO @ Sun, 07 Oct 2018 20:53:35: 15000000 INFO @ Sun, 07 Oct 2018 20:53:35: 16000000 INFO @ Sun, 07 Oct 2018 20:53:42: 17000000 INFO @ Sun, 07 Oct 2018 20:53:42: 18000000 INFO @ Sun, 07 Oct 2018 20:53:43: 16000000 INFO @ Sun, 07 Oct 2018 20:53:48: 18000000 INFO @ Sun, 07 Oct 2018 20:53:49: 19000000 INFO @ Sun, 07 Oct 2018 20:53:51: 17000000 INFO @ Sun, 07 Oct 2018 20:53:55: 19000000 INFO @ Sun, 07 Oct 2018 20:53:56: 20000000 INFO @ Sun, 07 Oct 2018 20:53:59: 18000000 INFO @ Sun, 07 Oct 2018 20:54:02: 20000000 INFO @ Sun, 07 Oct 2018 20:54:03: 21000000 INFO @ Sun, 07 Oct 2018 20:54:04: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:54:04: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:54:04: #1 total tags in treatment: 21166684 INFO @ Sun, 07 Oct 2018 20:54:04: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:54:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:54:05: #1 tags after filtering in treatment: 21166684 INFO @ Sun, 07 Oct 2018 20:54:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:54:05: #1 finished! INFO @ Sun, 07 Oct 2018 20:54:05: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:54:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:54:06: 19000000 INFO @ Sun, 07 Oct 2018 20:54:06: #2 number of paired peaks: 71 WARNING @ Sun, 07 Oct 2018 20:54:06: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:54:06: Process for pairing-model is terminated! cat: SRX4669069.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669069.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669069.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669069.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:54:08: 21000000 INFO @ Sun, 07 Oct 2018 20:54:10: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:54:10: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:54:10: #1 total tags in treatment: 21166684 INFO @ Sun, 07 Oct 2018 20:54:10: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:54:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:54:10: #1 tags after filtering in treatment: 21166684 INFO @ Sun, 07 Oct 2018 20:54:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:54:10: #1 finished! INFO @ Sun, 07 Oct 2018 20:54:10: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:54:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:54:11: #2 number of paired peaks: 71 WARNING @ Sun, 07 Oct 2018 20:54:11: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:54:11: Process for pairing-model is terminated! cat: SRX4669069.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669069.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669069.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669069.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 20:54:13: 20000000 INFO @ Sun, 07 Oct 2018 20:54:20: 21000000 INFO @ Sun, 07 Oct 2018 20:54:21: #1 tag size is determined as 50 bps INFO @ Sun, 07 Oct 2018 20:54:21: #1 tag size = 50 INFO @ Sun, 07 Oct 2018 20:54:21: #1 total tags in treatment: 21166684 INFO @ Sun, 07 Oct 2018 20:54:21: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 20:54:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 20:54:22: #1 tags after filtering in treatment: 21166684 INFO @ Sun, 07 Oct 2018 20:54:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 20:54:22: #1 finished! INFO @ Sun, 07 Oct 2018 20:54:22: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 20:54:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 20:54:23: #2 number of paired peaks: 71 WARNING @ Sun, 07 Oct 2018 20:54:23: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 20:54:23: Process for pairing-model is terminated! cat: SRX4669069.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4669069.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669069.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4669069.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。