Job ID = 6528114 SRX = SRX4669061 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:26:36 prefetch.2.10.7: 1) Downloading 'SRR7817586'... 2020-06-29T14:26:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:28:34 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:28:35 prefetch.2.10.7: 'SRR7817586' is valid 2020-06-29T14:28:35 prefetch.2.10.7: 1) 'SRR7817586' was downloaded successfully 2020-06-29T14:28:35 prefetch.2.10.7: 'SRR7817586' has 0 unresolved dependencies Read 23934079 spots for SRR7817586/SRR7817586.sra Written 23934079 spots for SRR7817586/SRR7817586.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:20 23934079 reads; of these: 23934079 (100.00%) were unpaired; of these: 1107449 (4.63%) aligned 0 times 18977103 (79.29%) aligned exactly 1 time 3849527 (16.08%) aligned >1 times 95.37% overall alignment rate Time searching: 00:06:21 Overall time: 00:06:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5112546 / 22826630 = 0.2240 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:42: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:42: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:47: 1000000 INFO @ Mon, 29 Jun 2020 23:46:52: 2000000 INFO @ Mon, 29 Jun 2020 23:46:57: 3000000 INFO @ Mon, 29 Jun 2020 23:47:03: 4000000 INFO @ Mon, 29 Jun 2020 23:47:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:12: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:12: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:13: 6000000 INFO @ Mon, 29 Jun 2020 23:47:18: 1000000 INFO @ Mon, 29 Jun 2020 23:47:18: 7000000 INFO @ Mon, 29 Jun 2020 23:47:23: 8000000 INFO @ Mon, 29 Jun 2020 23:47:24: 2000000 INFO @ Mon, 29 Jun 2020 23:47:28: 9000000 INFO @ Mon, 29 Jun 2020 23:47:29: 3000000 INFO @ Mon, 29 Jun 2020 23:47:34: 10000000 INFO @ Mon, 29 Jun 2020 23:47:35: 4000000 INFO @ Mon, 29 Jun 2020 23:47:39: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:41: 5000000 INFO @ Mon, 29 Jun 2020 23:47:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:42: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:42: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:44: 12000000 INFO @ Mon, 29 Jun 2020 23:47:47: 6000000 INFO @ Mon, 29 Jun 2020 23:47:48: 1000000 INFO @ Mon, 29 Jun 2020 23:47:49: 13000000 INFO @ Mon, 29 Jun 2020 23:47:53: 7000000 INFO @ Mon, 29 Jun 2020 23:47:54: 2000000 INFO @ Mon, 29 Jun 2020 23:47:54: 14000000 INFO @ Mon, 29 Jun 2020 23:47:59: 15000000 INFO @ Mon, 29 Jun 2020 23:47:59: 8000000 INFO @ Mon, 29 Jun 2020 23:48:00: 3000000 INFO @ Mon, 29 Jun 2020 23:48:04: 16000000 INFO @ Mon, 29 Jun 2020 23:48:05: 9000000 INFO @ Mon, 29 Jun 2020 23:48:06: 4000000 INFO @ Mon, 29 Jun 2020 23:48:09: 17000000 INFO @ Mon, 29 Jun 2020 23:48:11: 10000000 INFO @ Mon, 29 Jun 2020 23:48:12: 5000000 INFO @ Mon, 29 Jun 2020 23:48:13: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:48:13: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:48:13: #1 total tags in treatment: 17714084 INFO @ Mon, 29 Jun 2020 23:48:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:48:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:48:13: #1 tags after filtering in treatment: 17714084 INFO @ Mon, 29 Jun 2020 23:48:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:48:13: #1 finished! INFO @ Mon, 29 Jun 2020 23:48:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:48:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:48:14: #2 number of paired peaks: 83 WARNING @ Mon, 29 Jun 2020 23:48:14: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:48:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:48:17: 11000000 INFO @ Mon, 29 Jun 2020 23:48:17: 6000000 INFO @ Mon, 29 Jun 2020 23:48:22: 12000000 INFO @ Mon, 29 Jun 2020 23:48:23: 7000000 INFO @ Mon, 29 Jun 2020 23:48:28: 13000000 INFO @ Mon, 29 Jun 2020 23:48:29: 8000000 INFO @ Mon, 29 Jun 2020 23:48:34: 14000000 INFO @ Mon, 29 Jun 2020 23:48:35: 9000000 INFO @ Mon, 29 Jun 2020 23:48:40: 15000000 INFO @ Mon, 29 Jun 2020 23:48:40: 10000000 INFO @ Mon, 29 Jun 2020 23:48:45: 16000000 INFO @ Mon, 29 Jun 2020 23:48:46: 11000000 INFO @ Mon, 29 Jun 2020 23:48:51: 17000000 INFO @ Mon, 29 Jun 2020 23:48:52: 12000000 INFO @ Mon, 29 Jun 2020 23:48:55: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:48:55: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:48:55: #1 total tags in treatment: 17714084 INFO @ Mon, 29 Jun 2020 23:48:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:48:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:48:55: #1 tags after filtering in treatment: 17714084 INFO @ Mon, 29 Jun 2020 23:48:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:48:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:48:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:48:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:48:56: #2 number of paired peaks: 83 WARNING @ Mon, 29 Jun 2020 23:48:56: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:48:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:48:57: 13000000 INFO @ Mon, 29 Jun 2020 23:49:03: 14000000 INFO @ Mon, 29 Jun 2020 23:49:08: 15000000 INFO @ Mon, 29 Jun 2020 23:49:14: 16000000 INFO @ Mon, 29 Jun 2020 23:49:19: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:49:23: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:49:23: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:49:23: #1 total tags in treatment: 17714084 INFO @ Mon, 29 Jun 2020 23:49:23: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:49:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:49:23: #1 tags after filtering in treatment: 17714084 INFO @ Mon, 29 Jun 2020 23:49:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:49:23: #1 finished! INFO @ Mon, 29 Jun 2020 23:49:23: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:49:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:49:24: #2 number of paired peaks: 83 WARNING @ Mon, 29 Jun 2020 23:49:24: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:49:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669061/SRX4669061.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。