Job ID = 6528113 SRX = SRX4669059 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:18:35 prefetch.2.10.7: 1) Downloading 'SRR7817584'... 2020-06-29T14:18:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:19:44 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:19:44 prefetch.2.10.7: 'SRR7817584' is valid 2020-06-29T14:19:44 prefetch.2.10.7: 1) 'SRR7817584' was downloaded successfully 2020-06-29T14:19:44 prefetch.2.10.7: 'SRR7817584' has 0 unresolved dependencies Read 18498291 spots for SRR7817584/SRR7817584.sra Written 18498291 spots for SRR7817584/SRR7817584.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:43 18498291 reads; of these: 18498291 (100.00%) were unpaired; of these: 792654 (4.29%) aligned 0 times 15116268 (81.72%) aligned exactly 1 time 2589369 (14.00%) aligned >1 times 95.71% overall alignment rate Time searching: 00:04:43 Overall time: 00:04:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2474710 / 17705637 = 0.1398 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:32:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:32:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:32:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:32:52: 1000000 INFO @ Mon, 29 Jun 2020 23:32:57: 2000000 INFO @ Mon, 29 Jun 2020 23:33:02: 3000000 INFO @ Mon, 29 Jun 2020 23:33:06: 4000000 INFO @ Mon, 29 Jun 2020 23:33:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:33:15: 6000000 INFO @ Mon, 29 Jun 2020 23:33:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:33:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:33:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:33:20: 7000000 INFO @ Mon, 29 Jun 2020 23:33:23: 1000000 INFO @ Mon, 29 Jun 2020 23:33:25: 8000000 INFO @ Mon, 29 Jun 2020 23:33:28: 2000000 INFO @ Mon, 29 Jun 2020 23:33:30: 9000000 INFO @ Mon, 29 Jun 2020 23:33:33: 3000000 INFO @ Mon, 29 Jun 2020 23:33:35: 10000000 INFO @ Mon, 29 Jun 2020 23:33:37: 4000000 INFO @ Mon, 29 Jun 2020 23:33:40: 11000000 INFO @ Mon, 29 Jun 2020 23:33:42: 5000000 INFO @ Mon, 29 Jun 2020 23:33:44: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:33:47: 6000000 INFO @ Mon, 29 Jun 2020 23:33:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:33:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:33:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:33:49: 13000000 INFO @ Mon, 29 Jun 2020 23:33:52: 7000000 INFO @ Mon, 29 Jun 2020 23:33:53: 1000000 INFO @ Mon, 29 Jun 2020 23:33:54: 14000000 INFO @ Mon, 29 Jun 2020 23:33:57: 8000000 INFO @ Mon, 29 Jun 2020 23:33:58: 2000000 INFO @ Mon, 29 Jun 2020 23:33:59: 15000000 INFO @ Mon, 29 Jun 2020 23:34:01: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:34:01: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:34:01: #1 total tags in treatment: 15230927 INFO @ Mon, 29 Jun 2020 23:34:01: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:34:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:34:01: #1 tags after filtering in treatment: 15230927 INFO @ Mon, 29 Jun 2020 23:34:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:34:01: #1 finished! INFO @ Mon, 29 Jun 2020 23:34:01: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:34:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:34:01: 9000000 INFO @ Mon, 29 Jun 2020 23:34:02: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:34:02: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:34:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:34:03: 3000000 INFO @ Mon, 29 Jun 2020 23:34:06: 10000000 INFO @ Mon, 29 Jun 2020 23:34:08: 4000000 INFO @ Mon, 29 Jun 2020 23:34:11: 11000000 INFO @ Mon, 29 Jun 2020 23:34:13: 5000000 INFO @ Mon, 29 Jun 2020 23:34:16: 12000000 INFO @ Mon, 29 Jun 2020 23:34:18: 6000000 INFO @ Mon, 29 Jun 2020 23:34:22: 13000000 INFO @ Mon, 29 Jun 2020 23:34:23: 7000000 INFO @ Mon, 29 Jun 2020 23:34:27: 14000000 INFO @ Mon, 29 Jun 2020 23:34:28: 8000000 INFO @ Mon, 29 Jun 2020 23:34:32: 15000000 INFO @ Mon, 29 Jun 2020 23:34:33: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:34:33: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:34:33: #1 total tags in treatment: 15230927 INFO @ Mon, 29 Jun 2020 23:34:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:34:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:34:33: 9000000 INFO @ Mon, 29 Jun 2020 23:34:33: #1 tags after filtering in treatment: 15230927 INFO @ Mon, 29 Jun 2020 23:34:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:34:33: #1 finished! INFO @ Mon, 29 Jun 2020 23:34:33: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:34:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:34:34: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:34:34: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:34:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:34:38: 10000000 INFO @ Mon, 29 Jun 2020 23:34:43: 11000000 INFO @ Mon, 29 Jun 2020 23:34:48: 12000000 INFO @ Mon, 29 Jun 2020 23:34:53: 13000000 INFO @ Mon, 29 Jun 2020 23:34:58: 14000000 INFO @ Mon, 29 Jun 2020 23:35:03: 15000000 INFO @ Mon, 29 Jun 2020 23:35:05: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:35:05: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:35:05: #1 total tags in treatment: 15230927 INFO @ Mon, 29 Jun 2020 23:35:05: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:35:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:35:05: #1 tags after filtering in treatment: 15230927 INFO @ Mon, 29 Jun 2020 23:35:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:35:05: #1 finished! INFO @ Mon, 29 Jun 2020 23:35:05: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:35:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:35:06: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:35:06: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:35:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4669059/SRX4669059.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。