Job ID = 12265244 SRX = SRX4664623 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 44164996 spots for SRR7813050/SRR7813050.sra Written 44164996 spots for SRR7813050/SRR7813050.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265702 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:22:32 44164996 reads; of these: 44164996 (100.00%) were paired; of these: 12827547 (29.04%) aligned concordantly 0 times 15287857 (34.62%) aligned concordantly exactly 1 time 16049592 (36.34%) aligned concordantly >1 times ---- 12827547 pairs aligned concordantly 0 times; of these: 4265891 (33.26%) aligned discordantly 1 time ---- 8561656 pairs aligned 0 times concordantly or discordantly; of these: 17123312 mates make up the pairs; of these: 10917348 (63.76%) aligned 0 times 1547438 (9.04%) aligned exactly 1 time 4658526 (27.21%) aligned >1 times 87.64% overall alignment rate Time searching: 01:22:32 Overall time: 01:22:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 14474021 / 35045576 = 0.4130 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:02:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:02:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:02:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:02:41: 1000000 INFO @ Sat, 03 Apr 2021 08:02:47: 2000000 INFO @ Sat, 03 Apr 2021 08:02:53: 3000000 INFO @ Sat, 03 Apr 2021 08:03:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:03:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:03:04: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:03:04: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:03:06: 5000000 INFO @ Sat, 03 Apr 2021 08:03:10: 1000000 INFO @ Sat, 03 Apr 2021 08:03:13: 6000000 INFO @ Sat, 03 Apr 2021 08:03:16: 2000000 INFO @ Sat, 03 Apr 2021 08:03:19: 7000000 INFO @ Sat, 03 Apr 2021 08:03:22: 3000000 INFO @ Sat, 03 Apr 2021 08:03:26: 8000000 INFO @ Sat, 03 Apr 2021 08:03:28: 4000000 INFO @ Sat, 03 Apr 2021 08:03:32: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:03:33: 5000000 INFO @ Sat, 03 Apr 2021 08:03:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:03:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:03:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:03:38: 10000000 INFO @ Sat, 03 Apr 2021 08:03:39: 6000000 INFO @ Sat, 03 Apr 2021 08:03:41: 1000000 INFO @ Sat, 03 Apr 2021 08:03:45: 7000000 INFO @ Sat, 03 Apr 2021 08:03:45: 11000000 INFO @ Sat, 03 Apr 2021 08:03:48: 2000000 INFO @ Sat, 03 Apr 2021 08:03:50: 8000000 INFO @ Sat, 03 Apr 2021 08:03:51: 12000000 INFO @ Sat, 03 Apr 2021 08:03:54: 3000000 INFO @ Sat, 03 Apr 2021 08:03:55: 9000000 INFO @ Sat, 03 Apr 2021 08:03:58: 13000000 INFO @ Sat, 03 Apr 2021 08:04:01: 4000000 INFO @ Sat, 03 Apr 2021 08:04:01: 10000000 INFO @ Sat, 03 Apr 2021 08:04:05: 14000000 INFO @ Sat, 03 Apr 2021 08:04:07: 11000000 INFO @ Sat, 03 Apr 2021 08:04:08: 5000000 INFO @ Sat, 03 Apr 2021 08:04:11: 15000000 INFO @ Sat, 03 Apr 2021 08:04:12: 12000000 INFO @ Sat, 03 Apr 2021 08:04:14: 6000000 INFO @ Sat, 03 Apr 2021 08:04:18: 16000000 INFO @ Sat, 03 Apr 2021 08:04:18: 13000000 INFO @ Sat, 03 Apr 2021 08:04:21: 7000000 INFO @ Sat, 03 Apr 2021 08:04:24: 14000000 INFO @ Sat, 03 Apr 2021 08:04:24: 17000000 INFO @ Sat, 03 Apr 2021 08:04:27: 8000000 INFO @ Sat, 03 Apr 2021 08:04:29: 15000000 INFO @ Sat, 03 Apr 2021 08:04:31: 18000000 INFO @ Sat, 03 Apr 2021 08:04:34: 9000000 INFO @ Sat, 03 Apr 2021 08:04:35: 16000000 INFO @ Sat, 03 Apr 2021 08:04:37: 19000000 INFO @ Sat, 03 Apr 2021 08:04:40: 10000000 INFO @ Sat, 03 Apr 2021 08:04:41: 17000000 INFO @ Sat, 03 Apr 2021 08:04:44: 20000000 INFO @ Sat, 03 Apr 2021 08:04:47: 18000000 INFO @ Sat, 03 Apr 2021 08:04:47: 11000000 INFO @ Sat, 03 Apr 2021 08:04:50: 21000000 INFO @ Sat, 03 Apr 2021 08:04:52: 19000000 INFO @ Sat, 03 Apr 2021 08:04:53: 12000000 INFO @ Sat, 03 Apr 2021 08:04:57: 22000000 INFO @ Sat, 03 Apr 2021 08:04:57: 20000000 INFO @ Sat, 03 Apr 2021 08:05:00: 13000000 INFO @ Sat, 03 Apr 2021 08:05:02: 21000000 INFO @ Sat, 03 Apr 2021 08:05:03: 23000000 INFO @ Sat, 03 Apr 2021 08:05:07: 14000000 INFO @ Sat, 03 Apr 2021 08:05:07: 22000000 INFO @ Sat, 03 Apr 2021 08:05:10: 24000000 INFO @ Sat, 03 Apr 2021 08:05:12: 23000000 INFO @ Sat, 03 Apr 2021 08:05:13: 15000000 INFO @ Sat, 03 Apr 2021 08:05:16: 25000000 INFO @ Sat, 03 Apr 2021 08:05:16: 24000000 INFO @ Sat, 03 Apr 2021 08:05:20: 16000000 INFO @ Sat, 03 Apr 2021 08:05:21: 25000000 INFO @ Sat, 03 Apr 2021 08:05:23: 26000000 INFO @ Sat, 03 Apr 2021 08:05:26: 17000000 INFO @ Sat, 03 Apr 2021 08:05:26: 26000000 INFO @ Sat, 03 Apr 2021 08:05:29: 27000000 INFO @ Sat, 03 Apr 2021 08:05:31: 27000000 INFO @ Sat, 03 Apr 2021 08:05:33: 18000000 INFO @ Sat, 03 Apr 2021 08:05:36: 28000000 INFO @ Sat, 03 Apr 2021 08:05:36: 28000000 INFO @ Sat, 03 Apr 2021 08:05:39: 19000000 INFO @ Sat, 03 Apr 2021 08:05:41: 29000000 INFO @ Sat, 03 Apr 2021 08:05:42: 29000000 INFO @ Sat, 03 Apr 2021 08:05:45: 20000000 INFO @ Sat, 03 Apr 2021 08:05:45: 30000000 INFO @ Sat, 03 Apr 2021 08:05:48: 30000000 INFO @ Sat, 03 Apr 2021 08:05:50: 31000000 INFO @ Sat, 03 Apr 2021 08:05:51: 21000000 INFO @ Sat, 03 Apr 2021 08:05:54: 31000000 INFO @ Sat, 03 Apr 2021 08:05:55: 32000000 INFO @ Sat, 03 Apr 2021 08:05:58: 22000000 INFO @ Sat, 03 Apr 2021 08:05:59: 33000000 INFO @ Sat, 03 Apr 2021 08:06:00: 32000000 INFO @ Sat, 03 Apr 2021 08:06:04: 23000000 INFO @ Sat, 03 Apr 2021 08:06:04: 34000000 INFO @ Sat, 03 Apr 2021 08:06:06: 33000000 INFO @ Sat, 03 Apr 2021 08:06:09: 35000000 INFO @ Sat, 03 Apr 2021 08:06:10: 24000000 INFO @ Sat, 03 Apr 2021 08:06:12: 34000000 INFO @ Sat, 03 Apr 2021 08:06:14: 36000000 INFO @ Sat, 03 Apr 2021 08:06:16: 25000000 INFO @ Sat, 03 Apr 2021 08:06:18: 35000000 INFO @ Sat, 03 Apr 2021 08:06:19: 37000000 INFO @ Sat, 03 Apr 2021 08:06:22: 26000000 INFO @ Sat, 03 Apr 2021 08:06:24: 36000000 INFO @ Sat, 03 Apr 2021 08:06:24: 38000000 INFO @ Sat, 03 Apr 2021 08:06:28: 27000000 INFO @ Sat, 03 Apr 2021 08:06:29: 39000000 INFO @ Sat, 03 Apr 2021 08:06:30: 37000000 INFO @ Sat, 03 Apr 2021 08:06:34: 28000000 INFO @ Sat, 03 Apr 2021 08:06:34: 40000000 INFO @ Sat, 03 Apr 2021 08:06:36: 38000000 INFO @ Sat, 03 Apr 2021 08:06:39: 41000000 INFO @ Sat, 03 Apr 2021 08:06:39: 29000000 INFO @ Sat, 03 Apr 2021 08:06:41: 39000000 INFO @ Sat, 03 Apr 2021 08:06:44: 42000000 INFO @ Sat, 03 Apr 2021 08:06:44: 30000000 INFO @ Sat, 03 Apr 2021 08:06:47: 40000000 INFO @ Sat, 03 Apr 2021 08:06:49: 43000000 INFO @ Sat, 03 Apr 2021 08:06:49: 31000000 INFO @ Sat, 03 Apr 2021 08:06:53: 41000000 INFO @ Sat, 03 Apr 2021 08:06:54: 44000000 INFO @ Sat, 03 Apr 2021 08:06:55: 32000000 INFO @ Sat, 03 Apr 2021 08:06:58: 42000000 INFO @ Sat, 03 Apr 2021 08:06:59: 45000000 INFO @ Sat, 03 Apr 2021 08:07:00: 33000000 INFO @ Sat, 03 Apr 2021 08:07:03: 46000000 INFO @ Sat, 03 Apr 2021 08:07:04: 43000000 INFO @ Sat, 03 Apr 2021 08:07:05: 34000000 INFO @ Sat, 03 Apr 2021 08:07:08: 47000000 INFO @ Sat, 03 Apr 2021 08:07:09: 44000000 INFO @ Sat, 03 Apr 2021 08:07:10: 35000000 INFO @ Sat, 03 Apr 2021 08:07:13: 48000000 INFO @ Sat, 03 Apr 2021 08:07:15: 45000000 INFO @ Sat, 03 Apr 2021 08:07:16: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:07:16: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:07:16: #1 total tags in treatment: 18075897 INFO @ Sat, 03 Apr 2021 08:07:16: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:07:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:07:16: #1 tags after filtering in treatment: 13762151 INFO @ Sat, 03 Apr 2021 08:07:16: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 03 Apr 2021 08:07:16: #1 finished! INFO @ Sat, 03 Apr 2021 08:07:16: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:07:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:07:16: 36000000 INFO @ Sat, 03 Apr 2021 08:07:17: #2 number of paired peaks: 142 WARNING @ Sat, 03 Apr 2021 08:07:17: Fewer paired peaks (142) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 142 pairs to build model! INFO @ Sat, 03 Apr 2021 08:07:17: start model_add_line... INFO @ Sat, 03 Apr 2021 08:07:17: start X-correlation... INFO @ Sat, 03 Apr 2021 08:07:17: end of X-cor INFO @ Sat, 03 Apr 2021 08:07:17: #2 finished! INFO @ Sat, 03 Apr 2021 08:07:17: #2 predicted fragment length is 56 bps INFO @ Sat, 03 Apr 2021 08:07:17: #2 alternative fragment length(s) may be 3,56,77,565,597 bps INFO @ Sat, 03 Apr 2021 08:07:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.10_model.r WARNING @ Sat, 03 Apr 2021 08:07:17: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:07:17: #2 You may need to consider one of the other alternative d(s): 3,56,77,565,597 WARNING @ Sat, 03 Apr 2021 08:07:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:07:17: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:07:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:07:20: 46000000 INFO @ Sat, 03 Apr 2021 08:07:21: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:07:26: 47000000 INFO @ Sat, 03 Apr 2021 08:07:27: 38000000 INFO @ Sat, 03 Apr 2021 08:07:31: 48000000 INFO @ Sat, 03 Apr 2021 08:07:32: 39000000 INFO @ Sat, 03 Apr 2021 08:07:34: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:07:34: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:07:34: #1 total tags in treatment: 18075897 INFO @ Sat, 03 Apr 2021 08:07:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:07:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:07:34: #1 tags after filtering in treatment: 13762151 INFO @ Sat, 03 Apr 2021 08:07:34: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 03 Apr 2021 08:07:34: #1 finished! INFO @ Sat, 03 Apr 2021 08:07:34: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:07:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:07:35: #2 number of paired peaks: 142 WARNING @ Sat, 03 Apr 2021 08:07:35: Fewer paired peaks (142) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 142 pairs to build model! INFO @ Sat, 03 Apr 2021 08:07:35: start model_add_line... INFO @ Sat, 03 Apr 2021 08:07:35: start X-correlation... INFO @ Sat, 03 Apr 2021 08:07:35: end of X-cor INFO @ Sat, 03 Apr 2021 08:07:35: #2 finished! INFO @ Sat, 03 Apr 2021 08:07:35: #2 predicted fragment length is 56 bps INFO @ Sat, 03 Apr 2021 08:07:35: #2 alternative fragment length(s) may be 3,56,77,565,597 bps INFO @ Sat, 03 Apr 2021 08:07:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.05_model.r WARNING @ Sat, 03 Apr 2021 08:07:35: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:07:35: #2 You may need to consider one of the other alternative d(s): 3,56,77,565,597 WARNING @ Sat, 03 Apr 2021 08:07:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:07:35: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:07:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:07:37: 40000000 INFO @ Sat, 03 Apr 2021 08:07:41: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:07:42: 41000000 INFO @ Sat, 03 Apr 2021 08:07:47: 42000000 INFO @ Sat, 03 Apr 2021 08:07:52: 43000000 INFO @ Sat, 03 Apr 2021 08:07:54: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:07:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:07:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.10_summits.bed INFO @ Sat, 03 Apr 2021 08:07:54: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1239 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:07:57: 44000000 INFO @ Sat, 03 Apr 2021 08:07:59: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:08:02: 45000000 INFO @ Sat, 03 Apr 2021 08:08:07: 46000000 INFO @ Sat, 03 Apr 2021 08:08:11: 47000000 INFO @ Sat, 03 Apr 2021 08:08:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:08:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:08:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.05_summits.bed INFO @ Sat, 03 Apr 2021 08:08:13: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3689 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:08:16: 48000000 INFO @ Sat, 03 Apr 2021 08:08:19: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:08:19: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:08:19: #1 total tags in treatment: 18075897 INFO @ Sat, 03 Apr 2021 08:08:19: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:08:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:08:19: #1 tags after filtering in treatment: 13762151 INFO @ Sat, 03 Apr 2021 08:08:19: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 03 Apr 2021 08:08:19: #1 finished! INFO @ Sat, 03 Apr 2021 08:08:19: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:08:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:08:20: #2 number of paired peaks: 142 WARNING @ Sat, 03 Apr 2021 08:08:20: Fewer paired peaks (142) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 142 pairs to build model! INFO @ Sat, 03 Apr 2021 08:08:20: start model_add_line... INFO @ Sat, 03 Apr 2021 08:08:20: start X-correlation... INFO @ Sat, 03 Apr 2021 08:08:20: end of X-cor INFO @ Sat, 03 Apr 2021 08:08:20: #2 finished! INFO @ Sat, 03 Apr 2021 08:08:20: #2 predicted fragment length is 56 bps INFO @ Sat, 03 Apr 2021 08:08:20: #2 alternative fragment length(s) may be 3,56,77,565,597 bps INFO @ Sat, 03 Apr 2021 08:08:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.20_model.r WARNING @ Sat, 03 Apr 2021 08:08:20: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:08:20: #2 You may need to consider one of the other alternative d(s): 3,56,77,565,597 WARNING @ Sat, 03 Apr 2021 08:08:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:08:20: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:08:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:08:44: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:08:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:08:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:08:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4664623/SRX4664623.20_summits.bed INFO @ Sat, 03 Apr 2021 08:08:58: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (356 records, 4 fields): 2 millis CompletedMACS2peakCalling