Job ID = 1298042 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 38,541,369 reads read : 77,082,738 reads written : 77,082,738 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:53 38541369 reads; of these: 38541369 (100.00%) were paired; of these: 36542631 (94.81%) aligned concordantly 0 times 1595295 (4.14%) aligned concordantly exactly 1 time 403443 (1.05%) aligned concordantly >1 times ---- 36542631 pairs aligned concordantly 0 times; of these: 7303 (0.02%) aligned discordantly 1 time ---- 36535328 pairs aligned 0 times concordantly or discordantly; of these: 73070656 mates make up the pairs; of these: 72104452 (98.68%) aligned 0 times 369387 (0.51%) aligned exactly 1 time 596817 (0.82%) aligned >1 times 6.46% overall alignment rate Time searching: 00:13:53 Overall time: 00:13:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 102531 / 2000143 = 0.0513 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 17:38:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:38:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:38:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:38:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:38:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:38:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:38:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 17:38:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 17:38:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 17:38:23: 1000000 INFO @ Mon, 03 Jun 2019 17:38:23: 1000000 INFO @ Mon, 03 Jun 2019 17:38:25: 1000000 INFO @ Mon, 03 Jun 2019 17:38:32: 2000000 INFO @ Mon, 03 Jun 2019 17:38:32: 2000000 INFO @ Mon, 03 Jun 2019 17:38:34: 2000000 INFO @ Mon, 03 Jun 2019 17:38:40: 3000000 INFO @ Mon, 03 Jun 2019 17:38:41: 3000000 INFO @ Mon, 03 Jun 2019 17:38:43: 3000000 INFO @ Mon, 03 Jun 2019 17:38:48: 4000000 INFO @ Mon, 03 Jun 2019 17:38:49: 4000000 INFO @ Mon, 03 Jun 2019 17:38:51: 4000000 INFO @ Mon, 03 Jun 2019 17:38:54: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 17:38:54: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 17:38:54: #1 total tags in treatment: 1896379 INFO @ Mon, 03 Jun 2019 17:38:54: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:38:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:38:54: #1 tags after filtering in treatment: 1821765 INFO @ Mon, 03 Jun 2019 17:38:54: #1 Redundant rate of treatment: 0.04 INFO @ Mon, 03 Jun 2019 17:38:54: #1 finished! INFO @ Mon, 03 Jun 2019 17:38:54: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:38:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:38:54: #2 number of paired peaks: 2150 INFO @ Mon, 03 Jun 2019 17:38:54: start model_add_line... INFO @ Mon, 03 Jun 2019 17:38:54: start X-correlation... INFO @ Mon, 03 Jun 2019 17:38:54: end of X-cor INFO @ Mon, 03 Jun 2019 17:38:54: #2 finished! INFO @ Mon, 03 Jun 2019 17:38:54: #2 predicted fragment length is 146 bps INFO @ Mon, 03 Jun 2019 17:38:54: #2 alternative fragment length(s) may be 4,146 bps INFO @ Mon, 03 Jun 2019 17:38:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.10_model.r INFO @ Mon, 03 Jun 2019 17:38:54: #3 Call peaks... INFO @ Mon, 03 Jun 2019 17:38:54: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 17:38:56: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 17:38:56: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 17:38:56: #1 total tags in treatment: 1896379 INFO @ Mon, 03 Jun 2019 17:38:56: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:38:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:38:56: #1 tags after filtering in treatment: 1821765 INFO @ Mon, 03 Jun 2019 17:38:56: #1 Redundant rate of treatment: 0.04 INFO @ Mon, 03 Jun 2019 17:38:56: #1 finished! INFO @ Mon, 03 Jun 2019 17:38:56: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:38:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:38:56: #2 number of paired peaks: 2150 INFO @ Mon, 03 Jun 2019 17:38:56: start model_add_line... INFO @ Mon, 03 Jun 2019 17:38:56: start X-correlation... INFO @ Mon, 03 Jun 2019 17:38:56: end of X-cor INFO @ Mon, 03 Jun 2019 17:38:56: #2 finished! INFO @ Mon, 03 Jun 2019 17:38:56: #2 predicted fragment length is 146 bps INFO @ Mon, 03 Jun 2019 17:38:56: #2 alternative fragment length(s) may be 4,146 bps INFO @ Mon, 03 Jun 2019 17:38:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.05_model.r INFO @ Mon, 03 Jun 2019 17:38:56: #3 Call peaks... INFO @ Mon, 03 Jun 2019 17:38:56: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 17:38:58: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 17:38:58: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 17:38:58: #1 total tags in treatment: 1896379 INFO @ Mon, 03 Jun 2019 17:38:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 17:38:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 17:38:58: #1 tags after filtering in treatment: 1821765 INFO @ Mon, 03 Jun 2019 17:38:58: #1 Redundant rate of treatment: 0.04 INFO @ Mon, 03 Jun 2019 17:38:58: #1 finished! INFO @ Mon, 03 Jun 2019 17:38:58: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 17:38:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 17:38:58: #2 number of paired peaks: 2150 INFO @ Mon, 03 Jun 2019 17:38:58: start model_add_line... INFO @ Mon, 03 Jun 2019 17:38:58: start X-correlation... INFO @ Mon, 03 Jun 2019 17:38:58: end of X-cor INFO @ Mon, 03 Jun 2019 17:38:58: #2 finished! INFO @ Mon, 03 Jun 2019 17:38:58: #2 predicted fragment length is 146 bps INFO @ Mon, 03 Jun 2019 17:38:58: #2 alternative fragment length(s) may be 4,146 bps INFO @ Mon, 03 Jun 2019 17:38:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.20_model.r INFO @ Mon, 03 Jun 2019 17:38:58: #3 Call peaks... INFO @ Mon, 03 Jun 2019 17:38:58: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 17:39:01: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 17:39:02: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 17:39:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.10_peaks.xls INFO @ Mon, 03 Jun 2019 17:39:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 17:39:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.10_summits.bed INFO @ Mon, 03 Jun 2019 17:39:04: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (144 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:39:05: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 17:39:06: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.05_peaks.xls INFO @ Mon, 03 Jun 2019 17:39:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 17:39:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.05_summits.bed INFO @ Mon, 03 Jun 2019 17:39:06: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (325 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 17:39:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.20_peaks.xls INFO @ Mon, 03 Jun 2019 17:39:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 17:39:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650882/SRX4650882.20_summits.bed INFO @ Mon, 03 Jun 2019 17:39:08: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (50 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。