Job ID = 1296456 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 10,616,256 reads read : 21,232,512 reads written : 21,232,512 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:46 10616256 reads; of these: 10616256 (100.00%) were paired; of these: 10354619 (97.54%) aligned concordantly 0 times 189508 (1.79%) aligned concordantly exactly 1 time 72129 (0.68%) aligned concordantly >1 times ---- 10354619 pairs aligned concordantly 0 times; of these: 1207 (0.01%) aligned discordantly 1 time ---- 10353412 pairs aligned 0 times concordantly or discordantly; of these: 20706824 mates make up the pairs; of these: 20439935 (98.71%) aligned 0 times 88701 (0.43%) aligned exactly 1 time 178188 (0.86%) aligned >1 times 3.73% overall alignment rate Time searching: 00:02:46 Overall time: 00:02:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 5614 / 261992 = 0.0214 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 16:26:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:26:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:26:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:26:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:26:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:26:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:26:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:26:34: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:26:34: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:26:40: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:26:40: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:26:40: #1 total tags in treatment: 256033 INFO @ Mon, 03 Jun 2019 16:26:40: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:26:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:26:40: #1 tags after filtering in treatment: 253210 INFO @ Mon, 03 Jun 2019 16:26:40: #1 Redundant rate of treatment: 0.01 INFO @ Mon, 03 Jun 2019 16:26:40: #1 finished! INFO @ Mon, 03 Jun 2019 16:26:40: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:26:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:26:40: #2 number of paired peaks: 1167 INFO @ Mon, 03 Jun 2019 16:26:40: start model_add_line... INFO @ Mon, 03 Jun 2019 16:26:40: start X-correlation... INFO @ Mon, 03 Jun 2019 16:26:40: end of X-cor INFO @ Mon, 03 Jun 2019 16:26:40: #2 finished! INFO @ Mon, 03 Jun 2019 16:26:40: #2 predicted fragment length is 97 bps INFO @ Mon, 03 Jun 2019 16:26:40: #2 alternative fragment length(s) may be 97 bps INFO @ Mon, 03 Jun 2019 16:26:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.20_model.r INFO @ Mon, 03 Jun 2019 16:26:40: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:26:40: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:26:41: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:26:41: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:26:41: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:26:41: #1 total tags in treatment: 256033 INFO @ Mon, 03 Jun 2019 16:26:41: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:26:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:26:41: #1 tags after filtering in treatment: 253210 INFO @ Mon, 03 Jun 2019 16:26:41: #1 Redundant rate of treatment: 0.01 INFO @ Mon, 03 Jun 2019 16:26:41: #1 finished! INFO @ Mon, 03 Jun 2019 16:26:41: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:26:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:26:41: #2 number of paired peaks: 1167 INFO @ Mon, 03 Jun 2019 16:26:41: start model_add_line... INFO @ Mon, 03 Jun 2019 16:26:41: start X-correlation... INFO @ Mon, 03 Jun 2019 16:26:41: end of X-cor INFO @ Mon, 03 Jun 2019 16:26:41: #2 finished! INFO @ Mon, 03 Jun 2019 16:26:41: #2 predicted fragment length is 97 bps INFO @ Mon, 03 Jun 2019 16:26:41: #2 alternative fragment length(s) may be 97 bps INFO @ Mon, 03 Jun 2019 16:26:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.05_model.r INFO @ Mon, 03 Jun 2019 16:26:41: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:26:41: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:26:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.20_peaks.xls INFO @ Mon, 03 Jun 2019 16:26:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:26:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.20_summits.bed INFO @ Mon, 03 Jun 2019 16:26:41: Done! pass1 - making usageList (3 chroms): 1 millis pass2 - checking and writing primary data (29 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 16:26:42: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:26:42: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:26:42: #1 total tags in treatment: 256033 INFO @ Mon, 03 Jun 2019 16:26:42: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:26:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:26:42: #1 tags after filtering in treatment: 253210 INFO @ Mon, 03 Jun 2019 16:26:42: #1 Redundant rate of treatment: 0.01 INFO @ Mon, 03 Jun 2019 16:26:42: #1 finished! INFO @ Mon, 03 Jun 2019 16:26:42: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:26:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:26:42: #2 number of paired peaks: 1167 INFO @ Mon, 03 Jun 2019 16:26:42: start model_add_line... INFO @ Mon, 03 Jun 2019 16:26:42: start X-correlation... INFO @ Mon, 03 Jun 2019 16:26:42: end of X-cor INFO @ Mon, 03 Jun 2019 16:26:42: #2 finished! INFO @ Mon, 03 Jun 2019 16:26:42: #2 predicted fragment length is 97 bps INFO @ Mon, 03 Jun 2019 16:26:42: #2 alternative fragment length(s) may be 97 bps INFO @ Mon, 03 Jun 2019 16:26:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.10_model.r INFO @ Mon, 03 Jun 2019 16:26:42: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:26:42: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:26:42: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:26:42: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.05_peaks.xls INFO @ Mon, 03 Jun 2019 16:26:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:26:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.05_summits.bed INFO @ Mon, 03 Jun 2019 16:26:42: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (82 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 03 Jun 2019 16:26:43: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:26:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.10_peaks.xls INFO @ Mon, 03 Jun 2019 16:26:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:26:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650780/SRX4650780.10_summits.bed INFO @ Mon, 03 Jun 2019 16:26:43: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (46 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。