Job ID = 1296413 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 11,386,246 reads read : 22,772,492 reads written : 22,772,492 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:34 11386246 reads; of these: 11386246 (100.00%) were paired; of these: 11105383 (97.53%) aligned concordantly 0 times 205232 (1.80%) aligned concordantly exactly 1 time 75631 (0.66%) aligned concordantly >1 times ---- 11105383 pairs aligned concordantly 0 times; of these: 878 (0.01%) aligned discordantly 1 time ---- 11104505 pairs aligned 0 times concordantly or discordantly; of these: 22209010 mates make up the pairs; of these: 21980090 (98.97%) aligned 0 times 74836 (0.34%) aligned exactly 1 time 154084 (0.69%) aligned >1 times 3.48% overall alignment rate Time searching: 00:02:35 Overall time: 00:02:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6847 / 280968 = 0.0244 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 16:11:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:11:56: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:11:56: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:11:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:11:56: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:11:56: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:11:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:11:56: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:11:56: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:12:03: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:12:03: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:12:03: #1 total tags in treatment: 274019 INFO @ Mon, 03 Jun 2019 16:12:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:12:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:12:03: #1 tags after filtering in treatment: 271273 INFO @ Mon, 03 Jun 2019 16:12:03: #1 Redundant rate of treatment: 0.01 INFO @ Mon, 03 Jun 2019 16:12:03: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:12:03: #1 finished! INFO @ Mon, 03 Jun 2019 16:12:03: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:12:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:12:03: #1 total tags in treatment: 274019 INFO @ Mon, 03 Jun 2019 16:12:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:12:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:12:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:12:03: #1 tags after filtering in treatment: 271273 INFO @ Mon, 03 Jun 2019 16:12:03: #1 Redundant rate of treatment: 0.01 INFO @ Mon, 03 Jun 2019 16:12:03: #1 finished! INFO @ Mon, 03 Jun 2019 16:12:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:12:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:12:03: #1 tag size is determined as 39 bps INFO @ Mon, 03 Jun 2019 16:12:03: #1 tag size = 39 INFO @ Mon, 03 Jun 2019 16:12:03: #1 total tags in treatment: 274019 INFO @ Mon, 03 Jun 2019 16:12:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:12:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:12:03: #1 tags after filtering in treatment: 271273 INFO @ Mon, 03 Jun 2019 16:12:03: #1 Redundant rate of treatment: 0.01 INFO @ Mon, 03 Jun 2019 16:12:03: #1 finished! INFO @ Mon, 03 Jun 2019 16:12:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:12:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:12:03: #2 number of paired peaks: 1378 INFO @ Mon, 03 Jun 2019 16:12:03: start model_add_line... INFO @ Mon, 03 Jun 2019 16:12:03: start X-correlation... INFO @ Mon, 03 Jun 2019 16:12:03: #2 number of paired peaks: 1378 INFO @ Mon, 03 Jun 2019 16:12:03: start model_add_line... INFO @ Mon, 03 Jun 2019 16:12:03: start X-correlation... INFO @ Mon, 03 Jun 2019 16:12:03: end of X-cor INFO @ Mon, 03 Jun 2019 16:12:03: #2 finished! INFO @ Mon, 03 Jun 2019 16:12:03: #2 predicted fragment length is 99 bps INFO @ Mon, 03 Jun 2019 16:12:03: #2 alternative fragment length(s) may be 99,177 bps INFO @ Mon, 03 Jun 2019 16:12:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.20_model.r INFO @ Mon, 03 Jun 2019 16:12:03: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:12:03: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:12:03: end of X-cor INFO @ Mon, 03 Jun 2019 16:12:03: #2 finished! INFO @ Mon, 03 Jun 2019 16:12:03: #2 predicted fragment length is 99 bps INFO @ Mon, 03 Jun 2019 16:12:03: #2 alternative fragment length(s) may be 99,177 bps INFO @ Mon, 03 Jun 2019 16:12:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.10_model.r INFO @ Mon, 03 Jun 2019 16:12:03: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:12:03: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:12:04: #2 number of paired peaks: 1378 INFO @ Mon, 03 Jun 2019 16:12:04: start model_add_line... INFO @ Mon, 03 Jun 2019 16:12:04: start X-correlation... INFO @ Mon, 03 Jun 2019 16:12:04: end of X-cor INFO @ Mon, 03 Jun 2019 16:12:04: #2 finished! INFO @ Mon, 03 Jun 2019 16:12:04: #2 predicted fragment length is 99 bps INFO @ Mon, 03 Jun 2019 16:12:04: #2 alternative fragment length(s) may be 99,177 bps INFO @ Mon, 03 Jun 2019 16:12:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.05_model.r INFO @ Mon, 03 Jun 2019 16:12:04: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:12:04: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 03 Jun 2019 16:12:04: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:12:04: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:12:04: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.20_peaks.xls INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.20_summits.bed INFO @ Mon, 03 Jun 2019 16:12:05: Done! INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.10_peaks.xls INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.10_summits.bed INFO @ Mon, 03 Jun 2019 16:12:05: Done! INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.05_peaks.xls INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:12:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX4650759/SRX4650759.05_summits.bed pass1 - making usageList (3 chroms): 1 millis pass2 - checking and writing primary data (23 records, 4 fields): 2 millis INFO @ Mon, 03 Jun 2019 16:12:05: Done! pass1 - making usageList (4 chroms): 1 millis pass2 - checking and writing primary data (41 records, 4 fields): 1 millis CompletedMACS2peakCalling CompletedMACS2peakCalling pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (85 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。